FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003619502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGA34.411764705882353No Hit
GAAATCCTGCAGCACGTCATCGACTACATCTTGGACCTGCAGATCGCCCT34.411764705882353No Hit
TGTACTACAAGCTCTCAAGTTCTTTCATGGGGAGTTTGTATCATGGTCAT22.941176470588235No Hit
GTACATGGGATGAACGACTGCTACTCCAAGCTCAAGGAGCTGGTGCCCAG22.941176470588235No Hit
CACTACAAGAGGACAGCGGCGACTACAGGAGGCGCCGAAGACGCTGCTGA22.941176470588235No Hit
GGACGCCGCTGACCACCCTCAACACGGATATCAGCATCCTGTCCTTGCAG22.941176470588235No Hit
GAATAAAAGAAATCATGAACACCGCTTATTCAGCCACACAGTGCTTTGCT22.941176470588235No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTT11.4705882352941175No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGAGAATA11.4705882352941175No Hit
TTCAGAAGCCTGCAAGGACAGGATGCTGATATCCGTGTTGAGGGTGGTCA11.4705882352941175No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCCGAGTACTGTT11.4705882352941175Clontech SMART CDS Primer II A (95% over 24bp)
CAAATGATCCACCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTG11.4705882352941175No Hit
ACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAA11.4705882352941175No Hit
GTTGATAATATGCCTTAAAAAGCATCAGTACAACCCCTTTACTTGCTACG11.4705882352941175No Hit
GTGCATGAGTAGGTGGCCTGCAGTAATGTTAGCGGTTAGGTGTACGGCCA11.4705882352941175No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11.4705882352941175Clontech SMART CDS Primer II A (96% over 26bp)
ATTCAATATAGTCTAGTGAAAGGCAGACGCGGTGGCTCATGCCTGTAATC11.4705882352941175No Hit
CTCTTTCCCTACACGACGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAA11.4705882352941175Illumina Single End PCR Primer 1 (96% over 27bp)
AGTTGGCGACGCTCAAGGGCGTGGTGCCATTCCCACCCGAGCCGCCGATG11.4705882352941175No Hit
CCCACACTCCATTGGTGTCTGATCACTGCAGGGATGCCTGCCTTAGTCAT11.4705882352941175No Hit
ATCATGAACACCGCTTATTCAGCCACACAGTGCTTTGCTGTCATTTGACA11.4705882352941175No Hit
AGTCTGGGTGACAGAGTGAGACCCTGTCTCCAAAAAAAAAAGGAAAGAAA11.4705882352941175No Hit
ACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAT11.4705882352941175No Hit
CCCCGGAAGCCGGATTGCCCAAGGGCTTCGTCCAGGAACACAACGCACAA11.4705882352941175No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA11.4705882352941175Clontech SMART CDS Primer II A (100% over 26bp)
GATCTGCCCCGACTCGTCGGCACTCCAGCCGTATTTGCTCATCCACACCT11.4705882352941175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT11.4705882352941175No Hit
AAATGCGCCGTTTGCAGGTGAATCGACGCTCAGTCTCAGTATAAGGAAAA11.4705882352941175No Hit
ATTTCAGCTCACTGAAACCTCGACCTACTGGGTTCAAGTGATCCGCCTCA11.4705882352941175No Hit
GAACTTAAGTGGATGTTTTGGTACAACTTATAGAAAAGGTAAAGGAAACC11.4705882352941175No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTAT11.4705882352941175No Hit
GGTCTCTGGTGATGCAGGCTGACAATAGTGGGATGCGAGTCCAGGGCGAT11.4705882352941175No Hit
ATTTTGGACACGCCATTCCGGAATAGATAACGGATTTATTACCGTTCGAG11.4705882352941175No Hit
ATACAAAAATTAGCCGGTCGTGGTGGCTAGCGCCTGTAATTCCAGCTACT11.4705882352941175No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCT11.4705882352941175No Hit
CTCCCAGCACTTTGGGAGGCCGAGGCAGGTAGGCCACAAAGTCAGGAGTT11.4705882352941175No Hit
GTACATGGGGAGGCGAGAGCTGCGAAAGGCGAGAGCTGCGAAGGGCCAGG11.4705882352941175No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.4705882352941175Clontech Universal Primer Mix Long (96% over 26bp)
ACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAA11.4705882352941175No Hit
CCACCAATAGTGTGGTGGAACTTGTGGCCCTTTCCAAGGCCACGGCTCTT11.4705882352941175No Hit
CGACTGCTACTCCAAGCTCAAGGAGCTGGTGCCCAGCATCCCCCAGAACA11.4705882352941175No Hit
GCTACAAAGACGTAGGACAAAGGGAAAGCTGGCCTAATGATTACTGCTGC11.4705882352941175No Hit
TTTAAGTGCTGAACTTATTTTTCAACCATTTCACAAGGAGGACAAGTTGA11.4705882352941175No Hit
CCCATGCTCTGTTTGAGATGGTTACGCAGCACCTCCACGCCTTCACCTGT11.4705882352941175No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT11.4705882352941175No Hit
GTAGAAACGTAGGAACAATTTCGTGAACGCAATCCGGAGTGCCCAACATG11.4705882352941175No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA11.4705882352941175No Hit
AGCTAGATATATATTAGGATGACTGCATTTGGATGAGTATTCTTTCCTGT11.4705882352941175No Hit
CCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATGCCGATGTT11.4705882352941175No Hit
GATTCCGTGAATTTGTTGTTGTTGTTGTTGTGCAAAGGATAAAAGAAATC11.4705882352941175No Hit
CCCATTTACCATGTGGACCCATTACAGTGGTATGTAATGCTTCATAACCA11.4705882352941175No Hit
TCCACATCCAGATACTACAGAAAGAGTGTTTCAAACCTGCTCTATGAAAG11.4705882352941175No Hit
TATCAACGCAGAGTACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAG11.4705882352941175No Hit
GGCTTCTGAATTCCCTTCTGAGTTAATGTCAAATGACAGCAAAGCACTGT11.4705882352941175No Hit
CCTGCAATCACTATTTACAGTTCTGGTTTGAGCTTTGTTTGAATTCCACT11.4705882352941175No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT11.4705882352941175Clontech SMART CDS Primer II A (100% over 26bp)
GTTCTCCAAGAAGCAAACTGGATTTTCTTCTCTACTTTCCAAAGCTACTT11.4705882352941175No Hit
GTCCAGGACGCCGCTGACCACCCTCAACACGGATATCAGCATCCTGTCCT11.4705882352941175No Hit
TTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACT11.4705882352941175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)