FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003619503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGTGGGTCTCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAAC34.411764705882353No Hit
CTGCGCTACCGCAGCGGCCCTTCAGTCAGCTCCGCGCCGTCCCCAGCAGA22.941176470588235No Hit
GACTAATATACAGCCAAATCACTCCCCAAGGAGACGGTGCAAATTCACAG22.941176470588235No Hit
GGACAGGATGCTGATATCCGTGTTGAGGGTGGTCAGCGGCGTCCTGGACG22.941176470588235No Hit
TTTTTTAGGTCCATTCAACTTGTCCTCCTTGTGAAATGGTTGAAAAATAA22.941176470588235No Hit
TTGTTGTGCAAAGGATAAAAGAAATTATGAACACCGCTTATTCAGCCACA22.941176470588235No Hit
GGGCCAGGCAGCGCCCCCGCGCGGGGCGGGGGGGGGGGGGCCCGCCCGGG11.4705882352941175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTT11.4705882352941175No Hit
CTTTGCAGACTTTGTAGCAGCAGCAAAATGTCTGTTGGGCACTCGGATGT11.4705882352941175No Hit
GTGTCAGCGTGTACGGCGGATTCGCGCAGGGCCGGGCGAACGGCGCCTCT11.4705882352941175No Hit
TCTTGGACCTGCAGATCGCCCTGGACTCGCATCCCACTATTGTCAGCCTG11.4705882352941175No Hit
ATTTAATAGAGATTTATCTCTGTTCTTTTATTTTTGCCAAGGAAAGTTTC11.4705882352941175No Hit
GTTCTTTTCCATCCATCCCCCTGAGTCAGTTGGTTGAAGGGAGTTATTTT11.4705882352941175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG11.4705882352941175No Hit
GTACTAACATGTTCATGCAGCACCTTCAGCTGGGTGTAACGACTTGCAGG11.4705882352941175No Hit
CCACTATTGTCAGCCTGCATCACCAGAGACCCGGGCAGAACCAGGCGTCC11.4705882352941175No Hit
AATCACAATCCTTTTGCTGTTGAGAGTCCTGAGAGTCACTTTTAAGCTGA11.4705882352941175No Hit
TTCCATGTTAAAACACCTTAGTACAGGCCAAGTACAGTGGCTCACGCCTG11.4705882352941175No Hit
TGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTG11.4705882352941175No Hit
GTTCTTTTTTTATTTGAGATGGAGTCTTGCTCTGTCGGCCAGGCTATAGT11.4705882352941175No Hit
GAAGTTAAAAAGAAGAGGTGGAACCGTCCCAAAATGTCCCTTGCTCAGAA11.4705882352941175No Hit
CCCGATATGTCAATGAATTTATTAAGCCCATTAAAGAAAAATTACAAAAA11.4705882352941175No Hit
TGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGC11.4705882352941175No Hit
GCTAGGGCTGCAATAATGAAGGGCAAGATGAAGTGAAAGGTAAAGAATCG11.4705882352941175No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.4705882352941175Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11.4705882352941175No Hit
GGTGACCACGTGACCAACTTGCGCAAGATGGGAGCGCCCGAATCTGGCTT11.4705882352941175No Hit
ATCAACGCAGAGTACATGGGATGAACGACTGCTACTCCAAGCTCAAGGAG11.4705882352941175No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAGGGGGTTTATACA11.4705882352941175No Hit
CGGCATTGCAGAAGAAAACAAGGCATTTAGGTTTTAGATCAGGTGTAAGA11.4705882352941175No Hit
GCCCATAGTCCAGTTGCTGCAGCAAAAGTTGCTTGAGTCTTCGATAGGAG11.4705882352941175No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGCGTTTTAAACA11.4705882352941175No Hit
GTACATGGGGCAGTGGTATCAACGCAACAAAAAAAAAAAAAAAAAAAAAA11.4705882352941175No Hit
TCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGG11.4705882352941175No Hit
TCCCCCAGAACAAGAAGGTGAGCAAGATGGAAATCCTGCAGCACGTCATC11.4705882352941175No Hit
GTGCAAGTGCATGATCGACCAGGCACATCAAGAAGAACGGCCAATCCGAC11.4705882352941175No Hit
GGAGCGAGCTGGACGCCACTGTGGGGCTCTGAGAGTCCTGAATTCTTACT11.4705882352941175No Hit
GTGCTTTGCTGTCATTTGACATTAACTCAGAAGGGAATTCAGAAGCCTGC11.4705882352941175No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.4705882352941175Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA11.4705882352941175No Hit
CTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAAATTAAGC11.4705882352941175No Hit
TCATGGTGCCGGGCTTGAAGTTCTCGCTGACGATGTCGGCGGTGGCGATG11.4705882352941175No Hit
CACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGA11.4705882352941175No Hit
GTATTAATTCGGGGCTCTGTAGTCCTTTCTCTCAATTTTCTTTTAAATAC11.4705882352941175No Hit
TCCCGGAGGATCGTTAGCTGGAAGAGGGCTTGCTGCTGACGGACTTCACC11.4705882352941175No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.4705882352941175No Hit
GGGTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTT11.4705882352941175No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACCAAGCC11.4705882352941175No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTATTTTTTTT11.4705882352941175Clontech SMART CDS Primer II A (96% over 26bp)
TCCTTCCCTGGGCATACAAAGAATCCTTGCCCTTCTTATACTGTGTCACT11.4705882352941175No Hit
GCACTTAAAAGATTCCGTGAATTTGTTGTTGTTGTTGTTGTGCAAAGAAT11.4705882352941175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT11.4705882352941175No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG11.4705882352941175Clontech Universal Primer Mix Long (96% over 26bp)
CCCTCAACACGGATATCAGCATCCTGTCCTTGCAGGCTTCTGAATTCCCT11.4705882352941175No Hit
CTATAGACATGCGCCACCATGCTTCACTAATTTTTTTTTTTTTTTTTGTA11.4705882352941175No Hit
GAACAAGAAGGTGAGCAAGATGGAAATCCTGCAGCACGTCATCGACTACA11.4705882352941175No Hit
AGCCACCACGACCGGCTAATTTTTGTATCTTTTTTAGTAGAGACGGGGTT11.4705882352941175No Hit
GCAAAGAATAAAAGAAATCATGAACACCGCTTATTCAGCCACACAGTGCT11.4705882352941175No Hit
GCTTTGATGCCTTTGGTGAAAAAGGGAATGTCTTCCCATAAAAACTAGAC11.4705882352941175No Hit
GCATATCCACATTGACGGAATGCCGCTGCATATCATCGATACCGCTGGGC11.4705882352941175No Hit
ACAAGAAGGTGAGCAAGATGGAAATCCTGCAGCACGTCATCGACTACATC11.4705882352941175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)