FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003619780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT64.651162790697675Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA53.875968992248062Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC53.875968992248062Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG43.10077519379845Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG43.10077519379845Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC32.3255813953488373Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC32.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT21.550387596899225Clontech Universal Primer Mix Long (96% over 26bp)
CCGTTGGTTTCGTTCACGATCCGTTCGGGGTGCAGGCGGTGCAGGTCGGC21.550387596899225No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGGTGAT21.550387596899225Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG21.550387596899225Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGG21.550387596899225Clontech SMART CDS Primer II A (100% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC21.550387596899225Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21.550387596899225Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21.550387596899225Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTGAGTTCTGGTGCCAAAGCCTGGAGCAAGCATTGCAGTGCAGAGCCCT21.550387596899225No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTCCTCTGCGTTGCT10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGACGCAGTGGTATCAACGCAGAGTACATGG10.7751937984496124Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTAGATGGGAAGCAGTGGTATCAACGCCGAGTACATGGGAAGCAGTGGTA10.7751937984496124Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAG10.7751937984496124Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATT10.7751937984496124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACAC10.7751937984496124No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACGC10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGATTCCCATG10.7751937984496124Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
CATGCAACAACGCCACTGGTAATAAAGCTTTGGAATGGGTGCTCATTCTA10.7751937984496124No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGT10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGGAC10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.7751937984496124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACTTTATTTTTTTATTTTTTTTTTTTTTTTAATTTTGTAAC10.7751937984496124No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAGCAG10.7751937984496124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTACCCATGTACTCTGCGGTGATACCACTGATTCCCATGCACTCTG10.7751937984496124Clontech SMARTer II A Oligonucleotide (95% over 22bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTATGCGTTGAT10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGGTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.7751937984496124Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACAGGGGAAGCAGTGGTATCAAC10.7751937984496124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGACTC10.7751937984496124No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACAGGGGAAGCAGTGGTATC10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCGATACCAC10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
CTCAGGGAAAATAAATAAATATCCCCAAGAGTCACCGGGTACTGTGCCCA10.7751937984496124No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAG10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCTTGTACTCTGCGTTGATAC10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGC10.7751937984496124Clontech SMART CDS Primer II A (100% over 26bp)
GCTTCCCATGTACTCTGCGTTGACACCACTGCTTCCCATGTACTCTGCGT10.7751937984496124Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGT10.7751937984496124No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC10.7751937984496124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAGAAGATGGCTGCCCCAGCAGTGTCCGGGCTCTCCCGGCAGGTGCGAT10.7751937984496124No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.7751937984496124Clontech Universal Primer Mix Long (96% over 25bp)
TCCCATGTACGCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.7751937984496124Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACAACTGCTTCCCATGTA10.7751937984496124Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGTTTAAAGAGGCCTCTTTCCGACTCTAGGCCACTGGACTCCCTATTTCC10.7751937984496124No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGGGGAAGC10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCT10.7751937984496124Clontech Universal Primer Mix Long (96% over 27bp)
TCCAAAGCGGAAGCGGACATCGGTACAACACCGGCGTCAAAAATCATAAA10.7751937984496124No Hit
GATACCACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCC10.7751937984496124Clontech Universal Primer Mix Long (96% over 27bp)
ACAATATCCTCCCCTGTAGGTCGGATAAGCGAAGCGCATCCGACAACTCA10.7751937984496124No Hit
GGACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACGCGGTGGTA10.7751937984496124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACCATCAACAGTCTCAAAACCAAGAATCCCAAACTGAAAATTCTCTTGT10.7751937984496124No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA10.7751937984496124Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7751937984496124No Hit
GTGCTGTCCCCGGCCGGAAGATCGGCGCGACATCGCAGTCATGCAGCAGA10.7751937984496124No Hit
GCACAGCAGTGCCACAAATAAGTTGCACTATCATTATTAACTTTGCATCT10.7751937984496124No Hit
CATGTCCTCTGCGTTGATACCACTGCTTCCCATTTACTCTGCGTTGATAC10.7751937984496124Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCATAATCACTGACCAGCCTTGACACTCCCCTTGCAAACTCTCCCAGCAC10.7751937984496124No Hit
GTACACCTCCTGGGGGCAGTTCATCACGCCCTCCAGCATCGGGTTGCCGG10.7751937984496124No Hit
GGCCTCCTACACCTACCTCTCTCTGGGCGTCTATTGCGACCGCGATGATG10.7751937984496124No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT10.7751937984496124No Hit
CTTGGGAAGCAGTTGTATCAAATCAGATAACATGGGAACCAGTCGTATCA10.7751937984496124No Hit
CCCCTGGGCGGCAGGGATGCACGGCCTGACGATGCCGTCGCTCCGCCGCC10.7751937984496124No Hit
GTATAAATGCAGTGCATAAACGGTTGGAGACCATCGGGCTTGCATTTTTT10.7751937984496124No Hit
GCGTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTGTTCGT10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGCT10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGA10.7751937984496124No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACATCTACCCAAGTACACTGAGATTAT10.7751937984496124Clontech SMART CDS Primer II A (95% over 21bp)
CCCATGTACGCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.7751937984496124Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTGATACCACAGCTTCC10.7751937984496124No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GCGCACATCCTGGCTCGCGGGCACGCGCACCGCCGTCACCAGCCGCATCC10.7751937984496124No Hit
GATCCCGATCTGCTCGCCGGCTCTGCCCTGTTAACCGCGCCCGATGGCGA10.7751937984496124No Hit
GGGGTGGACAGGTCGGCCTCGCCAACCAGGTGATGCGCCTCGGCTTCATC10.7751937984496124No Hit
GCAGAGTACATGGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.7751937984496124Clontech Universal Primer Mix Long (100% over 27bp)
TACGAGTTGAGAGCCATGTGGACGCCATTGTCAGTCTGTACCAAAGTCGG10.7751937984496124No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG10.7751937984496124No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.7751937984496124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAACAGCAGTGCCACAAATAAGTTGCACTATCATTAGTAACTTTGCATCT10.7751937984496124No Hit
CCATGGGAAGCAGTGGTATCAACGCAGAGTACCTGGGAAGCAGTGGTATC10.7751937984496124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
CCCGTCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGT10.7751937984496124No Hit
ATTCCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGC10.7751937984496124Clontech Universal Primer Mix Long (96% over 25bp)
CATGCGAAGCAGTGGTAACAAACCAGCCTACCAGGGAAGCCGTGGTATCA10.7751937984496124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)