FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003619781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA107.751937984496124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT107.751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG53.875968992248062Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC43.10077519379845Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA43.10077519379845Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG43.10077519379845Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA32.3255813953488373Clontech SMART CDS Primer II A (100% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG32.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT21.550387596899225Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT21.550387596899225Clontech Universal Primer Mix Long (100% over 23bp)
GCTTCCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT21.550387596899225Clontech Universal Primer Mix Long (96% over 26bp)
ATTTATATGAGTAATATCTTTTATGGTCATGCACCCTTGATGAGTGGTCC21.550387596899225No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC21.550387596899225Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG21.550387596899225Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21.550387596899225Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21.550387596899225Clontech Universal Primer Mix Long (96% over 26bp)
CTGCCGCGCCACCTGCAGATCATCGAGATGATCGACGCCGAGCATCGCGC21.550387596899225No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG21.550387596899225Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA21.550387596899225Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC21.550387596899225Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC21.550387596899225Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAGAGGAATGGGGAATTGCTGCTCGGAGAGATCCTGTGTGTGAGGCCCA21.550387596899225No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA21.550387596899225Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.7751937984496124No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
ATTCCAGCATGCGCCAATAATCGGGGAGTTTTTGGTCGAAGCCGCGGAAA10.7751937984496124No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.7751937984496124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACAAGGCGT10.7751937984496124No Hit
GTTCCCGTTCTCGCGTCTGGTGCACATCTGGAGCGTACCGGTGGAGTATC10.7751937984496124No Hit
GGTTTGAATGATTTCGAGCGACTCTTTATCGATCGGGGCGGCATAAAAGC10.7751937984496124No Hit
GTGGTTCCCCAGCACGGTGGTGCCCAAGCCAACCCTGTGACTGACATGTA10.7751937984496124No Hit
GTGGGGCGGCAACCGGCTGACGCGGTACGCACCTGATGGGCGCGTGCTGG10.7751937984496124No Hit
GCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCTCTTAGTCGTGCT10.7751937984496124No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCGACGCAGAGTACATGGGAAGCAGTGGTA10.7751937984496124Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT10.7751937984496124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.7751937984496124Clontech Universal Primer Mix Long (95% over 22bp)
TGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCATG10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTT10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GCCCAGCAACGCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTT10.7751937984496124No Hit
GCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGT10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
ACTCCAGCCTGGGCGACAGAGCAAGACTCTGTCTAAAAAAAAAAAAAAAA10.7751937984496124No Hit
CTTATGTGCCTACCTTATCTAAGAAACTTTAAATGTTAAGCCAATCGGAA10.7751937984496124No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCAATGTACTCTGCGTTGAT10.7751937984496124Clontech SMART CDS Primer II A (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGCAGAATAGGAATCAAAGCTGAGTCA10.7751937984496124No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAG10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
TCTGAAAGGCTTCTGCTAACTCATGAATCAACACAGTGAAATGAGTGTTT10.7751937984496124No Hit
GTTGTACGTCTCCACGAACCAACCGCGCGCGTCGCCGTGACGGCGGGGGG10.7751937984496124No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAATGCTTACC10.7751937984496124No Hit
GTACATGGAAGCAGTGGTAACAACGCAGAGTACATGGGAAGCAGTGGTAT10.7751937984496124Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGA10.7751937984496124No Hit
CTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCC10.7751937984496124No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GATTACATGGGAAGAATTGTAAACAACCAATATAAAATGGGAACCAGTGT10.7751937984496124No Hit
GCGCCATGATCAGGTCTCATCCGATTGCAGACGGCCGGCATCGGCCAGCG10.7751937984496124No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCTGTTACTCTGCGT10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG10.7751937984496124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCCAGAGAATCTCCACTGTGTCCTTAGCTCTGCACAGTCAGGGCTTCC10.7751937984496124No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTGCGTTGTTACCACTGCTTCCCA10.7751937984496124No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
CCCCCGGAACCCAAAGACTTTGGTTTCCGGGGGGAGTATGGTTGCAAAGC10.7751937984496124No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA10.7751937984496124Clontech Universal Primer Mix Long (96% over 25bp)
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTTG10.7751937984496124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTACCACGAGATTCGTACCGTCGCGACGTGCCGGATGACCGGGCTTGACG10.7751937984496124No Hit
CTATTTTACCTATGACCCGTGGATTGGCAAGTTATTGTATCTTGAGGACT10.7751937984496124No Hit
CTTAGGAAGCTCTTGAGGACAGTTTTTAATTCAGAGAGTGTGGCTTCTTC10.7751937984496124No Hit
GCTATCGGCCGGATGACGACCGACTCACCCCGGTTTCCCGCACCCGTCGG10.7751937984496124No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAAC10.7751937984496124No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.7751937984496124Clontech Universal Primer Mix Long (95% over 21bp)
GCTGTGGCATTGCCGTTACTGGCGGCAAGCTGCGCCAAGCCGGCGGCGCT10.7751937984496124No Hit
TGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAG10.7751937984496124Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGATACCACT10.7751937984496124Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTGGGTTGTTACCAATGGTTCCC10.7751937984496124No Hit
GCCGTGAGCACCCTGTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGC10.7751937984496124No Hit
ACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTT10.7751937984496124No Hit

[OK]Adapter Content

Adapter graph