FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003619792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240
Sequences flagged as poor quality0
Sequence length140
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG70.3125Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50.2232142857142857Clontech Universal Primer Mix Long (96% over 26bp)
GGTCGTGATCTCAGGGTCCTAGGATCGAGCCCCATGACAGGTGCTGTGCT40.17857142857142858No Hit
GGATAGTGGTTGGTGGCGGAAGCGGACGTGGAATCAGGGTGTGGTTCACA40.17857142857142858No Hit
TCCCTACGATAAGATGCGACCTGGTCTGAAATCATTGCTTTGTCAAAGGA40.17857142857142858No Hit
CTGCATTTGGTGTCCAGTAAAAACCTCTGGTGCCGTGAGATGAGATTGTG40.17857142857142858No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30.13392857142857142Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAAGGGAGGGCCAATCTAAAAGAACCACAAAGAAACAGACCTGAGGC30.13392857142857142No Hit
GCCTTGGACAAGTTCTGCAAGCTGGGCCACCCGGCTGCGGGGAATGGGGC30.13392857142857142No Hit
CACCAGATCCAGGGTAATCTCAGGTGCAGACCAGAATCACCAGGTCCACG30.13392857142857142No Hit
AGCATATCCTGTGTGACACTAGTGGGAGAAGCTTCTTAGAAGGGTGAGCC30.13392857142857142No Hit
GGAGAGAAAAAAGAAAAGAATGCCTGGGTGGCTCAGCAGTTGAGCGTCTG30.13392857142857142No Hit
AAATAACACTGGCCATTTATTCTGTTTTGTAATTCCATTTCAACTTATTG30.13392857142857142No Hit
CTTTAACACCGCCTCACAATCTTTCTCTGTCTTCATCTAAGCCTGCACCT30.13392857142857142No Hit
TCCATATTCTGTGTCAAAAGATCAGCCCTACTCCACCAGTTCCCTTTTCT30.13392857142857142No Hit
GATACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGA30.13392857142857142Illumina Single End PCR Primer 1 (100% over 34bp)
CCAGAGACCAGACAACACAGGACAGGTCCCAGCAGATGGGAGTCTCTGTT30.13392857142857142No Hit
AATGAGGTCTGCATTTCAGGAAGATCACTCCAGCAGCCAGATGAAAAACT30.13392857142857142No Hit
GCCAAAGGCAGGCGCCAAACCACTGAGCCACCCAGGGATCCCTGCATTCT30.13392857142857142No Hit
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA30.13392857142857142Illumina Single End PCR Primer 1 (100% over 21bp)
ACCCAGGGATCCCCCTAAATTTTTTTTTTTAAGATTTTATTTATTTATTA30.13392857142857142No Hit
GAGTCACATACTTCTTGTGCAAATATTCACACCTTTTTATCTCATTAAAA30.13392857142857142No Hit
GTGTGAGGCAGACTATAGGGACCTTTGGCGATTGTGTTGAGCCACCAAGA30.13392857142857142No Hit
GAAGGGAGCAGGAGCTGTCTGGCCCAGGGGGAGGCCCAGCGGCTGTCCCG30.13392857142857142No Hit
GGTAGAGGGTGAAATCGCACATAATGGAGGCTGGGAGCTGTGGAGTTTAA30.13392857142857142No Hit
AAATGTGCTATCAAAGAAATTCTAATCATTACACATCAACAGCCTACAGG30.13392857142857142No Hit
GCCCAGCATTCAGTAATTGATTACTTGAAGTTCATATTGAAGTTGGTACC30.13392857142857142No Hit
GATAAGAGCATATTGAGATGTTAAGACTATATGGAATCTCTAGAGAAGAG30.13392857142857142No Hit
CCTTTGCAGTTACTGATTCTACAAAACTGAGCTTATCGACTTTTCATCTT30.13392857142857142No Hit
TCATGGTAATACGTTAGCTGCTTGGCCGGAAAAGCTATATATAGTTTTCC30.13392857142857142No Hit
CTGTGCATGGGTCCTTTTATATAAAACACTGAAACATTCTACTGACCAGA30.13392857142857142No Hit
CATATAGATAGCTGTTAGATATCTCATTGAGAAAAATAATATACAGCCCA30.13392857142857142No Hit
GGCCCAGGTGAAGACCAGAGTTATAGGTCTGGATGGACACTATTAGTGAA30.13392857142857142No Hit
AAAGAAGGATGTCCGCTCTCACCACTGCTATTCAACATTGTGCTGCAAAT30.13392857142857142No Hit
ATCCAGATGAGAAGTGTCAGCCTAAGAGTGGTTACATAGAGAATGACCTG30.13392857142857142No Hit
CCGGCTGTCCCAGTAATGAAGGGACCACCCTCAGAGGAGACCTAAGTCAT30.13392857142857142No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30.13392857142857142Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTAAGTATGTGCAATTTTATTTGCCAATTATGGCAATAAGGCAGGGGA30.13392857142857142No Hit
TAGTTAGATGACAGGACAATTTTAAGAATTGAATGAGATAAGATAAATAA30.13392857142857142No Hit
ATGTGAATACCACATATTATTTGAATATACATTTTAAAATAGTTTATGTT30.13392857142857142No Hit
GGTAGTAGGAGTCTGAGGGTGAAATAGTGCGTGTGTCCGGGCCATCTGGA30.13392857142857142No Hit
CACCAACATCCCCTCCTGCTCCCAGCCCCCGGCAACCACTAGTCCAACTT30.13392857142857142No Hit
GTGCAGAGGGTCTGCCCAGCCTTGGCGATCAGGACCTCAGGACGTCCGTG30.13392857142857142No Hit
GAGTAGAGGAATCTGGGAGAGATTTATATAGTCCAGGTTTTGCTGATCAT30.13392857142857142No Hit
CTATTGTAATTGGTCTCATTCTTTCCCAGGTGAAAGAGACTATTTTCCCC30.13392857142857142No Hit
CTTTTGAAATCAGGAAAAATACAAGAGGATTAATAAATAACTCAGAGTAT30.13392857142857142No Hit
GGGCACCGTCGTTGCCATGAATCTGCAGGGCCTGCTTCACCGGCAGCAAC30.13392857142857142No Hit
ATATATTCCTATCGTGGATCAAATTCAAAATGTTGATCTAATAATACTGG30.13392857142857142No Hit
TGAATACAGAAAGAGTGAGTGAAATAGCAAGAGTTCTCTACTGCACAGAG30.13392857142857142No Hit
CTTCTGCTGGCTCCTCCTCTGCCCCTGATGTCTAACGGATTGCAGCTCAG30.13392857142857142No Hit
TCTCGGTGGTCGGTACACCCATGTACTCTGCGTTGATACCACTGCTTCCC30.13392857142857142Clontech Universal Primer Mix Long (96% over 26bp)
TCCCGTGAAGCTCCTTTGACACCAGGGTCCATTAAAGAGCTGTGGGGCCT30.13392857142857142No Hit
GTGCAGAAATGAACTATCCCCAATCCCTGCTCGTGGGTGACAGCTTATAG30.13392857142857142No Hit
TCATGACTCAGTCTCCCAAAACTGTGAAACGGGCGGGTGGATGAAATGGT30.13392857142857142No Hit
GGTCATGATCCTGGGGTCCTGGGATCGAGTCCCACATCAGGGTCCCCACG30.13392857142857142No Hit
GCTCTGATGCAATCACAACCCATTATTTTTGAGAGATCTTTATTATCAGA30.13392857142857142No Hit
AAGACAGCTTGAGGCTGTAGCACTCTTCCCACGTCAGCTGTGGCTGAATC30.13392857142857142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)