FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003619793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240
Sequences flagged as poor quality0
Sequence length140
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50.2232142857142857Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG50.2232142857142857Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCCCCAGGGCAGCCAATGCGGACTAGCAGGCTTAGAGGAAGGAAG40.17857142857142858No Hit
GTGGCAGGAAGTGGAGGGTATAGATGAAACGAGTGGCAGGATTATGATGG40.17857142857142858No Hit
CCTTTATATGTGTATAAATGCAAAGAACAATCTGGAAGGGTACTAAAAGA40.17857142857142858No Hit
GTATAGCAGAAGCTGTTCTTCTAGAGACAAAAACAAAAAACCTTTTGGGG40.17857142857142858No Hit
GCGCAGGGGTTTCGGTTGATGTGGACCGGACGAGTCCCCGGCAGGCGAGG30.13392857142857142No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30.13392857142857142Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTGGACATCTATAACCATCTCAACTAGTAACTCGTTGCTATATGACTTC30.13392857142857142No Hit
CCTGATCTTTCTCATACTCTAGAAACATTGTTTCCCCTTTGTGGGAATGC30.13392857142857142No Hit
GCCCCCTTCTACAGCTTCTGATGTGGAATCTAGGGCTGCTTTTTGTTATT30.13392857142857142No Hit
CAACCTTGACTGCTCTTCTTTAATAGAGTCAGACCCCTTTCAATGCACCT30.13392857142857142No Hit
CAGTAACGGCACAGGGGTTAGGAGCACTGACTCCCATGCAGTCAAAAATC30.13392857142857142No Hit
CTTCAAATAAGCAGTAGCTGAAAACAGTCCTATAACCTGGGGCTAAGAGA30.13392857142857142No Hit
CATTATAAGCTCCTTCAAGGCATGAACGGTCTCACTTGAACTGATGTTCT30.13392857142857142No Hit
CAATTTTCCTGGATCAATTTTGTACAGACCTAAAAACCTGCTGAGCATAT30.13392857142857142No Hit
GTCCTTGAAAATACAAGCTTATTAACTGCTGAATAAGTGAGGGAAAGAAT30.13392857142857142No Hit
GGACTGAGTCCCTCATGGGGCTCCCTGTGCGAGGTGTGCTTCTCCCTCTG30.13392857142857142No Hit
GTCCCAGCCCGATGGTGTAGGAGCTGAGGACCACCGGGCACTGGTTAGTT30.13392857142857142No Hit
ATGCTGAGTGGGTAGTCTCCTTGAGATTTTTTCTCTCTCTTCTTGTCATG30.13392857142857142No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGTACCGAC30.13392857142857142Clontech Universal Primer Mix Long (96% over 26bp)
ACCTTCTCCTGTGTGTCTGTATTTGTGTGTTTGTGTCTATGTTTTCCAAA30.13392857142857142No Hit
AAATCTAGGTCACTTTATCAAAGATAAAGATGGGAGATATAGCACACACA30.13392857142857142No Hit
CTCATGAGTAATGCAATCACAACTTTAAGTACTCTTAACAAATCAATTGA30.13392857142857142No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30.13392857142857142Clontech Universal Primer Mix Long (96% over 26bp)
ACACTGGAGGTAGGGCACATTGGTGCTTTTGACTGTGGAAGGAGTTATTT30.13392857142857142No Hit
TTTTTAAGATAAGGATTATATTACCCACCTTCTGGGGTTGTTAGAGGATG30.13392857142857142No Hit
ATTTATCACTGGTCACAGCCTAGATGCGAAAGCCATGAGCAGGTGAACTC30.13392857142857142No Hit
CTCTGGTTCTACCTGGGCATCATCGGGACATGGTTACTCTATTCCCCACT30.13392857142857142No Hit
GATAAAGGAATTTCATGCAATGAGAGTAGGATGGGGTTGGTGATTAAGGA30.13392857142857142No Hit
TAATTAGCCATTGACTGAGATGCGAAAAACCACAGGAAGTACAGTGTTTG30.13392857142857142No Hit
TTTCTTAGCTCACAACCTATTCTCAAAGAGCTCTAAATCTTCAAAGGCAG30.13392857142857142No Hit
GTATAAGTTAAAGTTATCTTTTTCTGATCATGTGAATATGGGCTTACACT30.13392857142857142No Hit
GCCCAAGTCCACACAGCCAGACACCAGGCCACATCCATCCTTCTGGGGTC30.13392857142857142No Hit
GGTTAGGGGTGCCGAATCCCTGTACCGCTGGAAATCCACACATGACTCGA30.13392857142857142No Hit
GAACACAGTAGATAATTAACAAATATTTCTCAAGTGAATGAGTAAACATA30.13392857142857142No Hit
AATTTAAGGAGAGTTAAAAAAGTCTAAATATACCAATAATCAGAAATAAT30.13392857142857142No Hit
ATCCACTTGCCAACACCACGTTGTCTTGATTACTGCAGTTTATGGTATAT30.13392857142857142No Hit
GTCCTCGTATATACATGTAGCTCACTTTTGTATGTTGACTTTGTGTCCTG30.13392857142857142No Hit
TGTCTGTTCAGAACTTTAGCCATTTGGACATCCTTTTAAGAGTGCAGCTT30.13392857142857142No Hit
GTTAGTGTTTGGACCGGCAGCATTTGGACAGAAGCAGAGCACATAAGCTT30.13392857142857142No Hit
GTGAACTACTTTGGGCTAATAGTTCATGTGGCATCTGAGAGATGTCAGAT30.13392857142857142No Hit
CTCATGAATAAATAAATAGAATCTTTAAAAAAAAATAGTGTGATCAGAGA30.13392857142857142No Hit
GTGATGTGGAACATTATGTTACATTGCATCCCGAAAATCTGTGCCAGTTT30.13392857142857142No Hit
CCACTACACCTCCCACTGCCCCTGGGCAAGTCTTTCCAGTCCACAGATGG30.13392857142857142No Hit
GCCCTTTGTCCAACAAGCTACATCCACAAAATCTAAGAACAAAAGCTGCC30.13392857142857142No Hit
GTGTAGTAATGTATAGAAGTCTAACTCAGAGTCTAATGAAAGAACATAAA30.13392857142857142No Hit
CTTCTGGAGTCCTTTGGCTTGCTTAAGGGCACTGTCAGACTGAGCCAAAG30.13392857142857142No Hit
TCCTGCGTCCAGTGGTGGGGCTGACACCTAGCAGGATCGAAATACTAGAA30.13392857142857142No Hit
GTGCTGACATTGGGGAGTCCAACACAGCCTAGACACGGAGGAGTGCCCAG30.13392857142857142No Hit
ATCAAAGCCCAAGTGACTAAAGAAAAACTTAAATAAATTGGGCTTTATTA30.13392857142857142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)