FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003619901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length140
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGCCCCGATCACCCTGGCCATCAACACGGATGCGTGCTCCGGCCTC33.614457831325301No Hit
CTTATGGCCCCATCACCGTGACCATCAACATGAAGCCCCTTCAGCTATAC22.4096385542168677No Hit
AATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCT22.4096385542168677No Hit
ATTCAAGATCAAGAGTAATTACCAACTTAATGTTTTTGCATTGGACTTTG22.4096385542168677No Hit
CTCCGCCATGACGGTGCAATCCTGTGGGCGAACGCTGCGCAGGCAACGCG22.4096385542168677No Hit
GTCTTAGAGGTGGGATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACC22.4096385542168677No Hit
GGTTTAGGTAACTGCGCAGCAACCTGCTCCACCTTCGCGGATTTTAGTTC22.4096385542168677No Hit
CCGCCCGCCCGCTCCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAAC22.4096385542168677No Hit
CTGCGAGGGATATGCCCTCCCCCACACCATCCTGCATCTGGACCTGGCTG22.4096385542168677No Hit
ATCTGTGGGACGCCTGGCGCGCGCTCAACGCCGCGCGGGAAGCGCGCGAC22.4096385542168677No Hit
CTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAAC11.2048192771084338No Hit
GCGGCACCGCCGAGCACGAGCACCGCGAGGCACATCACCGAGAGCGCGCG11.2048192771084338No Hit
CCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGA11.2048192771084338No Hit
TCCCTGGATTTTTGGAAAATCAAAAAGACCCTCTGGCTGTGGACAAAATA11.2048192771084338No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA11.2048192771084338No Hit
GCGGCCGAGGTGATCGACTTGCCGAGGCCGCCGACAGGCCCGCCGAAGTA11.2048192771084338No Hit
TCCGTGGACCCACGGCCCCGCTGGTCGGCGGCCCGGCATCAGCTCCGGCT11.2048192771084338No Hit
TGGACGCCTTGCTCGAGAAGCGCACTAGCGGGCTGATGAATCCAAAGCAG11.2048192771084338No Hit
ATTCCATTCCATTCCATTCCATTACATTCCACTTGATTCCTCTCCATTCC11.2048192771084338No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACCGGGTG11.2048192771084338No Hit
TTTCTATGTAGGATAGTGAAAAATCTTCAGAGCCAGAGTCCTGCATTCCA11.2048192771084338No Hit
GTGCCATAACCAACACGGCCAGCACCGCCACGGGGGTCACGATCGCCTTG11.2048192771084338No Hit
GTCATGTGATACGCGTCGCCCGTCAGGCCGAAGCCCGCGACCTCTGCATA11.2048192771084338No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG11.2048192771084338No Hit
CGCAAGAGGACGGCAGAGGCCCGGCAGCTCGGAGCTCCGGGACCTTGTGG11.2048192771084338No Hit
GTACTAGATTAACACTTTCATTTTACAGATGGGAAAAAAAAACAAAAACA11.2048192771084338No Hit
GAATAAAGACAATAGTAGCTGCTATAAAGGATGAGTTGGAAGTTATGTTA11.2048192771084338No Hit
GGCCAGCAACAATGCCCACGATGGGGACGGTGGACTGGGAAGATGGCTCC11.2048192771084338No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC11.2048192771084338No Hit
TGCCTGACCCTGGGCCCGGCGCTCAGGCTGACAGCCCAAATGGGCGGTAC11.2048192771084338No Hit
AGCAGCAGGAGCCGATGAAGACGGTGAAGGCCCTCGTCGGCGCGAACGTG11.2048192771084338No Hit
GCCTTTTGGTTTCTTATGTGTAGGAGAGGATAAGTTTCTTTCTCAGAGTG11.2048192771084338No Hit
CGAGCTCACCGTGCGCTCGGCCAACTGCCTGAAGGCCGAGAACATCTACT11.2048192771084338No Hit
CTCGACCATCGCGCCCGAAGACAGGAACATGCTGTGGCTGGTGTTCGCGA11.2048192771084338No Hit
ACCGAGCAGCAATCGAGTAAAACTCTTCCGCTCGGCATCGTAGACGTAGA11.2048192771084338No Hit
CAAATAGGTCATGTAAACAGTGACACGGGACCCATCGCAGGGCACACTGG11.2048192771084338No Hit
GGACGACCTGGTCCGCGCCCTGCACGCCCTGCTGACCGTGGCGGATCCGC11.2048192771084338No Hit
CCCAATACCCCATTCCATAGGATTAACAGCTAGTATATATGGTGGTGTGT11.2048192771084338No Hit
GTGTATACCTTCTACAGGTCCCCTCTGAGCCCTCTCACCTTGTCCTGTGG11.2048192771084338No Hit
GCGCAATCAAGCATTGTTTTGACGAACTTTCATTATGCGCACAAGACGCG11.2048192771084338No Hit
TCACCTACCTCCTCGCCATCGGCGTCGATCGCCTCCGCGACAAGGATCGG11.2048192771084338No Hit
GATCAGCCCCGTCGCAGCGCCGCCTCCAGCGCGGTCACGAAGCCGTCCAC11.2048192771084338No Hit
TTGCAACCCAGTTACAAAGCGGTTGCGCCCGCTTGGAATAGGCTTGCAAC11.2048192771084338No Hit
ATTTACAACCACAGAAAAACCTCTGTACATGTCTAGCGCCTGGCAGAGGG11.2048192771084338No Hit
CTGCTCCCCATTTTCCTCATTCTCAGCATTCCCGTTAGCAGGGGCGTCTC11.2048192771084338No Hit
GTGGAGGTATTGGCAGCCACCTGCAAGGACCCGAAGCTGTCCGCCTGCAG11.2048192771084338No Hit
CCACCTGGGCGTTCGAGAAGTACTGGATCGCCGACTCGTTCGAGGTCGTC11.2048192771084338No Hit
CCTCTAAGTCAGAATCCCGCCCAGGCGGAAAGATACGGCAGCGCCTGTGC11.2048192771084338No Hit
CCACTTAGGAGTCTGTTGTCATAATCCAAACGTGAGATGATGTGGGCCTT11.2048192771084338No Hit
CCACAAAGGTGAGGTTTAAAAGAAGTTTTCTCAGAATTTCAATGATCTTT11.2048192771084338No Hit
AGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCA11.2048192771084338No Hit
GTATACGACACTGGCGGAGGCCGAGGACCCGTCGCTGGCCACGGCGTTTG11.2048192771084338No Hit
AGGTGTAACGGGCTGGTTTGCCCAGTAAACACCATCTAGACACTGGAAAA11.2048192771084338No Hit
TTGCTGCCATGATCTGTCGTAAGTTTTGTTCCAGTTACTTGAGAGTTAAA11.2048192771084338No Hit
CGGTCGCGGCCTGCAGGAGGGCATGGCCCCAGGTAGGGGGACTGTTCTAG11.2048192771084338No Hit
ATACAGTACAGCTTCTTCTTTTCATCAGATCCATCATTGAAACGTTTCTG11.2048192771084338No Hit
ATTCTCTCCTGTACCCAGATGCAAAAAGTGCTAAGTTTCTCTTGTCCTCG11.2048192771084338No Hit
CAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAAGTTCCCCACGAACGT11.2048192771084338No Hit
GGGCCCACCCTCAGCTTGCGCAGCGCAAGCGATGGCGCGAGATCGGCAGC11.2048192771084338No Hit
CTCTGCTCACTGCAACCTCCACCTCCCGGGTTCAAGCAATTCTCCTGCCT11.2048192771084338No Hit
CACCCAGGCTCAGGGCACCTGTACAGAGCAAGAGGATAGCTGAGACCCAG11.2048192771084338No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAGATAAGCGTCCAGTTCAGCGCTGCCGCCCTCAGGCGCTGCTGGAT11.2048192771084338No Hit
CGGGAAACCCTTCCCGGTACCCCATCGCCACCCCTCAACAGAGCCCGCGG11.2048192771084338No Hit
AGACAAGAATGTCAAGAGTGTATTCTACCCCCACTGCCAGATGGAAGGGC11.2048192771084338No Hit
ACGACCCGTTCCACACCTACGAGGTGGATCTGGAGGTGCAGGACCTCGCC11.2048192771084338No Hit
GAGTCGCGCGGCCGTCGGGTAAGTGTGGTCCGGGAGGTTTTCGGACTTGG11.2048192771084338No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG11.2048192771084338No Hit
CGGCCGCGCGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGG11.2048192771084338No Hit
AAGCAACCTCGATCACCAAACGCTGCTCTGGCCCAGCTTCACCGGCGCAT11.2048192771084338No Hit
CTGCTGCAGGTTGTTCTGGAAGTTGAGGAAGGCCAGGTCTCCCTTCACCT11.2048192771084338No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)