FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003619914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23.9215686274509802Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATAGGACATTTGGCAGTATCTGGAAACATTCTTGATTTTCACGCTTG23.9215686274509802No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT23.9215686274509802Clontech Universal Primer Mix Long (100% over 23bp)
GGACCAGAGGCTTCTGAAGCTGTGAAGAAACTGCTGGAGGCGCTATCACA23.9215686274509802No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG23.9215686274509802Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTA23.9215686274509802No Hit
GATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCAT23.9215686274509802No Hit
CCATTAAAGCTTCTTCTGAAAACTCTGGGCATTTCTGTTGAGCACCTTGT23.9215686274509802No Hit
GGGCTATTTTTTCCTAAAACAAATAGACAAAGAAACAGAAAAACCCAATA23.9215686274509802No Hit
TTTTATAAGTTATCAAATATTTGGATAAAGAATGACTTGTAATTTTTTTA23.9215686274509802No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT23.9215686274509802No Hit
GCTCTTTGGGAGGCTGAGGCAGGCAGATCACAAGTTCAGGAGTTTGAGAC23.9215686274509802No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA11.9607843137254901Clontech Universal Primer Mix Long (95% over 22bp)
TGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGC11.9607843137254901No Hit
GCCTACATTTGTGCTCTCAATCACTGCTACTACTCAGGCTCTTGCCTTGA11.9607843137254901No Hit
TTGCTGTATGTGGTACGTGATCACTAACAGTGCTACAGCAAGGCACTTAA11.9607843137254901No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG11.9607843137254901No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCG11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
TCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCAC11.9607843137254901No Hit
GGGATATTTTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAAAACTCTG11.9607843137254901No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
CGCCCAGCCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCC11.9607843137254901No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACATGGGGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTA11.9607843137254901No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACTAAAAAATTTAAACACAATCCCTAAAACAAAAACAAAACAACTAACA11.9607843137254901No Hit
CCAAGCCAGTGGTTCCACTTGCCATACAAAGTGTCCCTTCCTGTTGACAA11.9607843137254901No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA11.9607843137254901No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT11.9607843137254901Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAA11.9607843137254901Clontech Universal Primer Mix Long (95% over 21bp)
TTCCTGGGCTGTGTCACTACAGCTCTGACTCCTTTCCACCTTGGAGTTTA11.9607843137254901No Hit
GCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
GTTTATAGTATTCTCTGATGGTAGTTTGTATTTCTGTGGGATTGGTGGCG11.9607843137254901No Hit
CCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAA11.9607843137254901No Hit
AGTCAGAACAGATTTGTTTTAGTGTGAGTAGAATGCATCCGAAGATGCCA11.9607843137254901No Hit
CTTTTCAATCAGGCTGCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCC11.9607843137254901No Hit
ACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGAAAGCTCG11.9607843137254901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)