FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003619915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35.88235294117647Clontech Universal Primer Mix Long (96% over 26bp)
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG35.88235294117647No Hit
TTCAGCGTGTCATCGAAACCCTCCTCATGGACACACCCTCCAGTTATGAG23.9215686274509802No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC23.9215686274509802Clontech Universal Primer Mix Long (96% over 26bp)
GCTCATTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATG23.9215686274509802No Hit
GCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACAC23.9215686274509802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23.9215686274509802No Hit
GAAAACATAGAAACATTTTTTCTCTAAAAATAAATGTAACCAAAAATCCA23.9215686274509802No Hit
CTGACACACTGTGATGGAAAAGGATGACTTGGCTCAGAGTTTGTGAAATG23.9215686274509802No Hit
GCAATGGTGTGATCTCTGCTCACTGCAACCTCCGCCTCCTGGGTTCAAGC23.9215686274509802No Hit
CATTCTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAAAACTCTGGGCA23.9215686274509802No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCT11.9607843137254901No Hit
CATTCAAAAGCTAGCAGAAGGCAAGAAATAACTAACATCAGAGCAGAACT11.9607843137254901No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
TGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACACCCTCCCCCAA11.9607843137254901No Hit
AGGGCAAACAAAACGTGAATTATATTAACATCTTGTCAAGAATAGCAGTG11.9607843137254901No Hit
AGAGGATACAGAAACAGGGAAAAGGAAAATGGATGAACAGAAAAAAAGAG11.9607843137254901No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG11.9607843137254901No Hit
CCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCGCTGAGCC11.9607843137254901No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11.9607843137254901Clontech Universal Primer Mix Long (100% over 23bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11.9607843137254901Clontech SMART CDS Primer II A (96% over 26bp)
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT11.9607843137254901No Hit
GAAGAGCGTCGTGTAGAAGAACCGTGTCCGCTCCCATGTACTCTGCGTTG11.9607843137254901No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT11.9607843137254901No Hit
GGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGAGAAGCCG11.9607843137254901No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.9607843137254901Clontech Universal Primer Mix Long (95% over 22bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
CCTGGCACCTGCTACTACATCTAGGACGCCTCCGGTGAGCAGGGTCAGTG11.9607843137254901No Hit
GCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
CATTCTTGTTGAGTCCCAGATACTTGGCCTGTTCATCTGTCAGCTCTGTC11.9607843137254901No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11.9607843137254901No Hit
CTACCAACTGGACCTATTTGCCTCAATTTCTTCATAAATTCATTCCTGAT11.9607843137254901No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
CCCTGCTGGTACCAGTAGACAGTGAGATCGGAAGAGCGTCGTGTAGGGAA11.9607843137254901Illumina Single End PCR Primer 1 (100% over 26bp)
TTTCTTCACAGCTTCAGAAGCCTCTGGTCCCAGCTCATTTACACACTTCC11.9607843137254901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)