| TCTCAGCCTCCCAGAGTGCTGGGATTACAGGCATCAGCCACCGTCCTGGC | 2 | 1.9801980198019802 | No Hit |
| GCCTTGTGGTTGTCCACTGCCAGGCAGGCATTTCCCGGTCAGCCACCATC | 2 | 1.9801980198019802 | No Hit |
| GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 2 | 1.9801980198019802 | No Hit |
| CAACATGGGTGGCTCTAGAGAATTCTGAGCCCCTGTGGCCCATGGAATGC | 2 | 1.9801980198019802 | No Hit |
| GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA | 2 | 1.9801980198019802 | Clontech SMARTer II A Oligonucleotide (100% over 25bp) |
| GGCTTCACAGAGGATACCATTGTCTTCCTGCCCCAAACCGATAAGTGCAT | 1 | 0.9900990099009901 | No Hit |
| GTAATGAAACATGAATTTAGTTTGGAAAATGGTTACAACCATCTAAACAA | 1 | 0.9900990099009901 | No Hit |
| CTAATGAAGTCTGCAGTTACAGACTCTCAAAGCCAAGCCTGTACAGTCTG | 1 | 0.9900990099009901 | No Hit |
| TTGTGTAGTAGTGCCAGCAGGACAAACAAGCGCTGAATTCAGTATATTCA | 1 | 0.9900990099009901 | No Hit |
| GTGGAATGCTGGGTGCTGGAAGGGAATCTCTTATTTTAAAAAGAAAAGAT | 1 | 0.9900990099009901 | No Hit |
| GGGCAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGAT | 1 | 0.9900990099009901 | No Hit |
| GGGGGGTCGAGTGTGAGTTGGACGGGTAACGACCGGGGGGTCCTGCATCA | 1 | 0.9900990099009901 | No Hit |
| GGGTTTCACCATATTGGCCAGGCTGGTCTTGAACTCCTGACCTCAAATGA | 1 | 0.9900990099009901 | No Hit |
| AGCAAATACAGCCAAAGGCTATTTACGGAGAAATTGAATTGCCAAAAGTC | 1 | 0.9900990099009901 | No Hit |
| CTATAAAGCCTGCTTGGATAATCAGCTAACCCTAATTCATCTCATTTCCT | 1 | 0.9900990099009901 | No Hit |
| GTGTCAACGCAGAGTACATGGGATGTGGAAATTGTTAAGGAAGTCAGCAC | 1 | 0.9900990099009901 | No Hit |
| GGGGGGGGGGGGTGGGGGGGGGGGGGGTGGGTGGGGGGGGTGGGTGGTGG | 1 | 0.9900990099009901 | No Hit |
| GCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGG | 1 | 0.9900990099009901 | No Hit |
| GGGGTGGGGGGGTGCGGGCGGGGTGGGGGTCGGTGGCGGCGCGGCTGGGG | 1 | 0.9900990099009901 | No Hit |
| TGATGGGGTTGTCCAGGCTGGTCTCAAACTCCTGAGCTCAAGCAATGTGC | 1 | 0.9900990099009901 | No Hit |
| GGGTCAGGGACATTCAAACCATAACACACACATTCTGATCTTTACCAAAT | 1 | 0.9900990099009901 | No Hit |
| GTTGAAGTGATTCTCCTGCCTCAGCCTCCCTAGTAGCTGGGACTACAGGC | 1 | 0.9900990099009901 | No Hit |
| GTTCAGGGACTCAGCAATGCACTTTAGGACTGAGCTAGGAGGCAAATATC | 1 | 0.9900990099009901 | No Hit |
| GGGGTGGCTGGGGGGGGTTGGAGGTGTAGCGTCCGGGGGGTGGTGCGGCG | 1 | 0.9900990099009901 | No Hit |
| ATGCTAAACGGCTTCCTTGGTACCCCGCTCACCAAAGCGTTCAGGATGGC | 1 | 0.9900990099009901 | No Hit |
| GTATAGACCACAGTTATTGGGGGAGAAGGGCTGGTAGGTTAAATTATCCT | 1 | 0.9900990099009901 | No Hit |
| CCTAAGGAATTCCCCTGCTTCAGCTCCTAGCTCAGCCACTGACAGCCCCT | 1 | 0.9900990099009901 | No Hit |
| TTAAGATTCTTGGTCTAAGGGGTAAGCAAATATCTTCCTGAGACTGGGAG | 1 | 0.9900990099009901 | No Hit |
| CTACAGGTATGTGCCACCACACCCAGCTTATCTTTGTATTGTTAGTAGAG | 1 | 0.9900990099009901 | No Hit |
| GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACGCTGCGG | 1 | 0.9900990099009901 | Clontech Universal Primer Mix Long (96% over 26bp) |
| CTTTAATTTCTGTGTGAGACCAAAGGAGGAGAGATGTGTTTTGTTCAAAA | 1 | 0.9900990099009901 | No Hit |
| CCCATGTACTCTGCGTTGATCCCACTGCTTCCCATGTACTCTGCGTTGAT | 1 | 0.9900990099009901 | Clontech SMARTer II A Oligonucleotide (96% over 25bp) |
| GCATCCATGTGTGGACTCAAGTCCTTACCTCTTCCGGAGATGTAGCAAAA | 1 | 0.9900990099009901 | No Hit |
| GTTTTCACACTGCTGTAAAGAACTGCTCAAGACTGGGTAATTTACGAAGA | 1 | 0.9900990099009901 | No Hit |
| CATATACCCAATGCCTGTACCCCCATTGTATCTTGGAAGTAACTAACTTG | 1 | 0.9900990099009901 | No Hit |
| GGTTATGAGCCTGCTGCAGCCCCTCAATATCACTCAGGTCCTGAGCCATG | 1 | 0.9900990099009901 | No Hit |
| GTGTATAAGCAGGAGTCATTCTTTACACAGTGTGTGCAATGAAGGGATGT | 1 | 0.9900990099009901 | No Hit |
| GAGGAATGCTGGGTGCTGGAAGGGAATCTCTTATTTTAAAAAGAAAAGAT | 1 | 0.9900990099009901 | No Hit |
| GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGTGGG | 1 | 0.9900990099009901 | No Hit |
| ATCCACCGGAGGGCAGCCGAGGTCACACTGGGGGCGCTGGCGCCATATGC | 1 | 0.9900990099009901 | No Hit |
| CCTCACCAGAGGTGCCACCTCCAACATCATAGTGGAGGCACTGAAAGACC | 1 | 0.9900990099009901 | No Hit |
| AAGCAATCCTCCTGTCTCAGCCTCCCGAATAGCTGCGACTACAGGTGCCC | 1 | 0.9900990099009901 | No Hit |
| CGATACAAGGGCACAAGTTTTCCAACTATGAACTCCTAACCTAATCGACT | 1 | 0.9900990099009901 | No Hit |
| AGGCGGCTCTTTGACCAAAGAGTCCCAATGTGAAATCCGTCTACAAAAAG | 1 | 0.9900990099009901 | No Hit |
| CTGCTACCTTCATCACCAAATTGCACTCGCTCCTTCCTGGAATCCTTTTT | 1 | 0.9900990099009901 | No Hit |
| GGGTTCAAAGAGCTCGTTGTCTCCCCATCTGCCGCTCCTGCACACGCGTG | 1 | 0.9900990099009901 | No Hit |
| GTCACCCAGGCTGGATGGAGTGCAGTGCCACAATCTCAGCGCGCTGCAAC | 1 | 0.9900990099009901 | No Hit |
| GGAAGCAGTGGTATCAACGCAGAGTAAATGGGAAGCAGTGGTATCAACGC | 1 | 0.9900990099009901 | Clontech Universal Primer Mix Long (96% over 25bp) |
| GCTTTGGAATCTTGGTAGTATGAAGCGGCCAGTCTGCAGGTCAGATTAAG | 1 | 0.9900990099009901 | No Hit |
| TGGTAGCGCATGCCTGTAATCCCAGCTACTAGGGAGGCTGAGGCAGGAGA | 1 | 0.9900990099009901 | No Hit |
| GTTGTTGGGGAGCTGTGGTGTCAACGGAGGGTGCATGGGAAGCAGTGGTA | 1 | 0.9900990099009901 | No Hit |
| GTTTTTGCCATGCTGCCCAGGCTGGTCTCAAACTCCTGGCCTCAAATGAT | 1 | 0.9900990099009901 | No Hit |
| GGCGCAGCTGCACCAACTATGTGGCGGGCAAGGATGACTATGTGAAGGCA | 1 | 0.9900990099009901 | No Hit |
| GGACGATGGATAACATGACTTCATCAGCACAGATAATTCAGAGATCTTTC | 1 | 0.9900990099009901 | No Hit |
| GGTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGT | 1 | 0.9900990099009901 | No Hit |
| GTTTAGAGCATTTCTTTGTTTGAAAAAAACTAATTTAGAAAATTTTTCAT | 1 | 0.9900990099009901 | No Hit |
| CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC | 1 | 0.9900990099009901 | Clontech Universal Primer Mix Long (96% over 26bp) |
| AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG | 1 | 0.9900990099009901 | No Hit |
| CACACACACACACACACACATACACACGAAGTAAGGATATCAACAATGCA | 1 | 0.9900990099009901 | No Hit |
| GGTGTTGCTCCATGGCCTGGGCTGGAGTATAGTGGTATGATCATAGCTCA | 1 | 0.9900990099009901 | No Hit |
| CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT | 1 | 0.9900990099009901 | Clontech Universal Primer Mix Long (96% over 26bp) |
| GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCT | 1 | 0.9900990099009901 | Clontech SMART CDS Primer II A (96% over 26bp) |
| GGGTATGAGCCTGCTGCAGCCCCTCAAAATCACTCAGGTCCTGAGCCATG | 1 | 0.9900990099009901 | No Hit |
| TGTATGTAAAGCATTCGGAAAAAAAGAATACTAAATTACTGTTGAAACTA | 1 | 0.9900990099009901 | No Hit |
| GGGGGGGGGGGGGGGTGTTTTGGTTTGTGTGGGGGGGGGGGGGGGGGTGC | 1 | 0.9900990099009901 | No Hit |
| GATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGAT | 1 | 0.9900990099009901 | No Hit |
| GGGCGGGGGGGGGGGCCGGGGGGGGGGGGGGGGGTGGGGGGGGGGTGGGT | 1 | 0.9900990099009901 | No Hit |
| GGGTTCCACGGTACTCACCACAGGGGAGTAAGTTGGTGGGAGGGATGCTG | 1 | 0.9900990099009901 | No Hit |
| AAGGGCAGGTGGGGCCAGGGGCTGGGCCCCTGGAACTGGAGGGAGTGAAG | 1 | 0.9900990099009901 | No Hit |
| GTACATGGGAAGGAAAAGATAAAAGGTTTCTAAAACATAACGGAGGTTGA | 1 | 0.9900990099009901 | No Hit |
| ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG | 1 | 0.9900990099009901 | Clontech Universal Primer Mix Long (96% over 26bp) |
| AAGGGCAGGTGGGGCCAGGGGCTGGGCCCCTGGAACTGGAGGGAGGGAAG | 1 | 0.9900990099009901 | No Hit |
| GCGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTTTTTT | 1 | 0.9900990099009901 | No Hit |
| GGGTCATAGACATTCAAACCATAACACACACATTCTGATCTTTACCAAAT | 1 | 0.9900990099009901 | No Hit |
| GCAGAGTACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC | 1 | 0.9900990099009901 | Clontech Universal Primer Mix Long (96% over 26bp) |
| CCCCAGTACTGATCCTAGAATAAACAGGGGGATAGCCGATCCCTGTCTCG | 1 | 0.9900990099009901 | No Hit |
| ATTTAATGGTGTTTTTATTTCCTGTCTGAAATCTCAAAATAAACAAACAT | 1 | 0.9900990099009901 | No Hit |
| CAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAAAT | 1 | 0.9900990099009901 | No Hit |
| GCCTAGTGGTTGTCCACTGCCAGGCAGGCATTTCCCGGTCAGCCACCATC | 1 | 0.9900990099009901 | No Hit |
| GGTTAGAGCATTTCTTTGTTTGAAAAAAACTAATTTAGAAACTTTTTCAT | 1 | 0.9900990099009901 | No Hit |
| CCAATGTACTCTGCCTAGATACCAAAGCTTCCCATGTACTCTGAATACAA | 1 | 0.9900990099009901 | No Hit |
| CAGATAGCAAGATGAAAACAAAATTTTTTTAAAAAAGAGAAATATAAACG | 1 | 0.9900990099009901 | No Hit |
| TGTTTGTACAGCTTTAGGAAATAAAGAATACTCAAATACTGTGGAAACTT | 1 | 0.9900990099009901 | No Hit |
| GGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGTGGGGGGGGGTGGTGGGT | 1 | 0.9900990099009901 | No Hit |
| CTATAAAGCCTGCTTGGATAATCAGCTTACCCTAATTCAGCTCTTTTCCT | 1 | 0.9900990099009901 | No Hit |
| GTTTTTGCCATGCTGCCCAGGCTGGTCGCAATCTCCTGGCCTCAAATGAA | 1 | 0.9900990099009901 | No Hit |
| GGGAAGCTGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAATAAAATAT | 1 | 0.9900990099009901 | No Hit |
| GCCTGGACTTGGCCCCTGCCACCTCCTGCCTCAGGTGTCCGCTATACACC | 1 | 0.9900990099009901 | No Hit |
| AGTTAGAACCAGATATGCTTTGTCTTGATTCTCAAGTAGAATAATCTTCA | 1 | 0.9900990099009901 | No Hit |
| CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC | 1 | 0.9900990099009901 | Clontech Universal Primer Mix Long (96% over 26bp) |
| CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG | 1 | 0.9900990099009901 | No Hit |
| GTGGGTGGGTAGGGGGGGTGTCAGCGCTGAGTTGAGGGGAGGGAGTGGTA | 1 | 0.9900990099009901 | No Hit |
| CATAAACCCAATGCCTGTACCCCCATTGTATCTTGGAAGTAACTAACTTG | 1 | 0.9900990099009901 | No Hit |
| GGGGTTCAGCATATTGGCCAGGCTGGTCTTGAACTCATGACCTCAAATGA | 1 | 0.9900990099009901 | No Hit |
| GGGTAGGTGGGTGTTGTATAAGGCTCTTAATAAGTCTACACTGTAGTAAT | 1 | 0.9900990099009901 | No Hit |
| GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT | 1 | 0.9900990099009901 | Clontech SMART CDS Primer II A (100% over 26bp) |
FastQC Report![[OK]](data:image/png;base64,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)
Basic Statistics
![[FAIL]](data:image/png;base64,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)
Per tile sequence quality
