FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003620019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003620019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA63.1088082901554404Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT42.072538860103627Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42.072538860103627Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG31.5544041450777202Clontech Universal Primer Mix Long (96% over 26bp)
GCCTTGTAGCGTGCCACGAGTTGCTGCACGAACGGGTCGGCCGGATAGAC31.5544041450777202No Hit
CCTGATGAGGATCCACCGGTGCCGCCGTCGATCTCCGTTCTGCGCTCAGT31.5544041450777202No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG31.5544041450777202Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG31.5544041450777202Clontech Universal Primer Mix Long (96% over 26bp)
TCCTTGGACGGTGCCACGGCCGGGTTGGCCGGGAATTCCTGGTTGACGCC31.5544041450777202No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31.5544041450777202Clontech Universal Primer Mix Long (96% over 26bp)
CAAGTACTCTGCGTTGATACCACTGCTTCCAAGTACTCTGCGTTGATACC21.0362694300518136Clontech SMART CDS Primer II A (100% over 26bp)
GTTCATATGTTGGACTTAATCCCCAGTGCAACAGTGTTGGGAGGTGGGGC21.0362694300518136No Hit
GTTCTGGTCGCCGGGGCAGGGCGTCTCCCCGGCGTGGCTCTACCCGGTCG21.0362694300518136No Hit
AATGAATGCAGGTGGTCTCCATTTTCTTCTCCTTTAGGCAACCATCTGAA21.0362694300518136No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG21.0362694300518136Clontech Universal Primer Mix Long (96% over 26bp)
CGGCCACGCTGGGCGTGTCCGCGGCCGACCGGCCGGCCAAGTTGGAGGAA21.0362694300518136No Hit
GGTTCTAGGGGTTAGGGTACTGATATCATTATGGGGAGCCATTATTCATC21.0362694300518136No Hit
GTACGCGATCGGTCTCGGCAGCGGTGCTCTCGTCGGCATCGCGACGGTGT21.0362694300518136No Hit
CTGAAGGCCGGTGGCCTCGATATCGTGTCGGGCGGTACGGATACACACCT21.0362694300518136No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG21.0362694300518136Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACATGGGATTGCTAATTTAGAGATAGGAAACTGAAGCATAAAGAATTA21.0362694300518136No Hit
GAAGACATGAGGCCGGTCACAGTGGTTCACGCCTGTAATCCCAGCACTTT21.0362694300518136No Hit
CGACAGCCGGTGCTTGCGTTTTACCGAAAAAAAAAAAAAAAAAAAAAAAA21.0362694300518136No Hit
GGCTACGCGCACGTCTTGTCGCCTCGCCGCGCCGCTTCTGGGGGCGATAT21.0362694300518136No Hit
GTAGTGGCATCATTCTATCACTCACTTGTTTAGCCAAGTTTTTGTCAATG21.0362694300518136No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGGGT21.0362694300518136Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG21.0362694300518136Clontech Universal Primer Mix Long (96% over 26bp)
TTCTTGTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGTGTACACCACC21.0362694300518136No Hit
ATTTGTACATTACCGTGTCTGTGGGTCAATTTAGCTGGATGCCTCTGACT21.0362694300518136No Hit
CTATGGCCATCAGCCAATACATCAAGGAAATCGGTCGCGGCCCGCGCGGC21.0362694300518136No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
GCACGTCGAGCAACTCGAGGAACGACATGTCCTCGTCGGCGTCGACCTCG10.5181347150259068No Hit
TCCGCGATGCGCGGATCGAGCGGCTGCGAACGCCAAGCGCGCCGGCCGAG10.5181347150259068No Hit
ATCACATTCCCAGAGCGTTGCGGCTTTATTACCGTGGAAACGGATAACGG10.5181347150259068No Hit
CCCTCGCGCAGCAGACTCACCGCGCAACCGGCATTCAGCGCGGCATCCTC10.5181347150259068No Hit
GCCTTTGGTCCTTGCAGCGCCGGCTGGCTCGCGCGCAAGGCGAGCAGGCG10.5181347150259068No Hit
CCCTCTACCTTGCGAATGTCATCCTGCAGGCGGCGGGCCTCGTCCATGCT10.5181347150259068No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
TCCTTGGCCTGACCCTCACACTCATCTAATGCTAAAGACCACCTGCAGAC10.5181347150259068No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.5181347150259068Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTC10.5181347150259068Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTCGAGGCTCTGCGGCAGTTCCTCGGGTGTCAGCGCCTGCGAGTCGTAG10.5181347150259068No Hit
GAATCCCACCGGAACCGCCGGCCACCCGGCGTCGTCCAAGCCGCATGACC10.5181347150259068No Hit
CCTCAGGACCCCGCTGTTTAGCAGGTGAGGCCCTAGGGCTGGCAACCACT10.5181347150259068No Hit
ATACAACACTGCTAATGAAAGCCCTCATTGCAACTCATTAGCTCTCACTC10.5181347150259068No Hit
ATCAACGCAGAGTACATGGGGTGTGCAGCAGCGGCGGCGGCGGTAGAGGC10.5181347150259068No Hit
GCGGTGAGCGTGCCGTGCCAGAGATTGCCCCAGATTTCGGCTGGTGGCAG10.5181347150259068No Hit
CTGCATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
CAGTAGCGGCGAGCAATCCTTCCGCCCTGGCGCTGCCGCTGCTGCGCGCC10.5181347150259068No Hit
GTCCCGCAGTGCTGGAATGGGCACGCGGGCTGCTGGAGACCGTGCGGTCC10.5181347150259068No Hit
ATGAAAGCGTTCCGCACCCCCGCTGTTGTCCTGGCGCTGACCTCTGGCCT10.5181347150259068No Hit
AAAGATGGAAGTCCTGCGTGGCTTAGGCGTCCCGTACACCGACGAGGATA10.5181347150259068No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
GCTCGAGCCCGAGCAGTCCCGCGCCGGTGACGCCGGGGCGACCCTGGTGC10.5181347150259068No Hit
TGCCGCACGAGAAGAGCCGGCGCATCGCCAAGGAAACCCTGGAAATCTAC10.5181347150259068No Hit
ATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10.5181347150259068No Hit
GAGTAGCTGGGAACACACGTGGATGCCACCATGACTGGCTAATTTTTTGT10.5181347150259068No Hit
GCTCTACTTCTCCGGCGACGACGCCATGGTGCTCCCGCACCTCAGCATCG10.5181347150259068No Hit
CTGCAATCTCCACCTCCTAGGTTCTAGCGATTCTCCTGCCTCAGCCTCCC10.5181347150259068No Hit
GACCCAAACACCTCCCACCAGGCCCTACCTCCAACACCGGGGGTCACATT10.5181347150259068No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTAATCTGCGTTGAT10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
ATAAGAACACTAATACAGCATTTTACCAAAGCGTACAAAGACAACTGCAA10.5181347150259068No Hit
ACTACGTGCAGCCCAACCTGCAGGTACAGCAGAAGAACCTCGACGCCAAC10.5181347150259068No Hit
GGGTTGGCTTCTGGCCATGGTGTGAGGCAGGGGTCCACCTTCACCACATG10.5181347150259068No Hit
AAAGTAGTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.5181347150259068Clontech SMART CDS Primer II A (96% over 26bp)
GTCATGTTCTAGTTGGCTTCAACTAGAAGCATTTCTCCCACTAGTCCATT10.5181347150259068No Hit
GCGGATGGGCTGCTCGATGAGGTCCATCAGCTGACTGGGGGCGTACGGCT10.5181347150259068No Hit
GGCGTCCCGCCCCATCTGAAAGACTTAGATTTAGCTGAGTGCAGCAGAGA10.5181347150259068No Hit
CCTCCATCTTCTGCCACAGGCTGATGGCTGCATTGGGGTCATAGCCTGCA10.5181347150259068No Hit
CTTTTAGGTTATGCAATTGGTGAAATAATTCGCAGAGGTCAATCATTTGA10.5181347150259068No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAGCAGTGG10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTCATGGGAAGCAGTGGTATCAACGCAGA10.5181347150259068Clontech Universal Primer Mix Long (95% over 24bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
CTCCCGGCTCAAGCAACCCTACACTGCAGCCTCCCGAGTCTGTCTCTTAT10.5181347150259068No Hit
CTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGC10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
GTTCACGAACTGCAGCGCGACGTCGAGCTCTTTGCCTTTGAGCAACGCGA10.5181347150259068No Hit
GCTGTGAGCGTGCCGTGCCAGAGATTGCCCCAGATTTCGGCTGGTGGCAG10.5181347150259068No Hit
CATGGGAAGCAGTGGTATCAACGTAGAGTACATGGGAAGCAGTGGTATCA10.5181347150259068Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTTCTGAGCGCCGTCCGCGATTATCTGGAGATCGTCGACACGAAGTTCAC10.5181347150259068No Hit
CCCATGTACTCTGCGTTGGTACCACTGCTTCCCATGTACTCTGCGTTGAT10.5181347150259068Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACCTATAACTTCACTCTGGAGTGAGCAGAAGGGGATTTACATTGAATCTT10.5181347150259068No Hit
TCGCGGGCCGCCGCGCCGGGGACGCGCGCTCGCCCGACCGGTGTGACACC10.5181347150259068No Hit
GTGCTGGAGCAACTCGCAGAACCGCAGCGCGCCGCCCATCTGCCACTGGA10.5181347150259068No Hit
CAGAAGAGATTACATCTAGGCGTATGCTCATTTCCAGACCTCCCTAAGAC10.5181347150259068No Hit
TATTCAACTTTCGAAGACTCCATTAAAGAGTGAAAGTTTTCTATTCCCAG10.5181347150259068No Hit
CCGGTCGACCTGGTCGAGTCGTTCGGCTATCCACTTCCGATCGCGGTGAT10.5181347150259068No Hit
CGTGGGCACCAGGGCGACACGCTCGGCAAGCTGCAATGACGCGAGGAACT10.5181347150259068No Hit
AACCAGGACAGTCTTGAAAAAGATTAATAAAGTTGAAAGATATATCCTAC10.5181347150259068No Hit
ACGCCCGCACTGTGTCGGTCGTGGTCAGCGAACGCACTCCGGAGTTCGCC10.5181347150259068No Hit
CGCATCGGCCATCGCCAGTCCATCGCGCTCGCCGAGAGCGAAGAGCGCCG10.5181347150259068No Hit
TCGAGGATCAGCAGCCGCACTTCCTTCGACAGCGCCTTGGCGATTTCCAC10.5181347150259068No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT10.5181347150259068Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTGAAAACAAGAAAAAGGATAAAAGCAAAACAAAGCATGCAATGTGAAA10.5181347150259068No Hit
CCACCATGTCGGTGCTCGGGCGATCCTCGCGCTCGCCGGCATCGGCCTCG10.5181347150259068No Hit
TGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATAAC10.5181347150259068No Hit
GCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCCG10.5181347150259068No Hit
CGGTCGCACCCTGGACGAACACCGGGGCATCGGTACCGCGCACCGGCAGC10.5181347150259068No Hit
AAGCAGGAAAGATCCAAAATTGACACCCTAACATCACAATTAAAAGAACT10.5181347150259068No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAATCAGCGT10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
ATCCAGGGCTTTGGGGCGGGTGCGGTCATGGTCCGTGCCTAGCAGGTTTC10.5181347150259068No Hit
AAGCAATGCCGCTCAGGTGCGGCACCAAACTTAAGTCGAGGCCGCGAGTT10.5181347150259068No Hit
AAGCAGTGGTATCAACGCGGAGTACATGGGCAGTGGTATCAACGCGGAGT10.5181347150259068Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTTTCGCCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCA10.5181347150259068No Hit
CCACCGGAGAAGGCCAGATCCTGCTTGTCGCCGAGGGCCTTGACCAGCAG10.5181347150259068No Hit
GGGCACTGGCGGGGGAGATCGACGGGCCACCGGTGAAGGCACTGATGGTC10.5181347150259068No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATACCAC10.5181347150259068No Hit
GCTCCCGTCCCACCCCACACCTCTCTCTCTCATCTTCTACTGTTAACAAG10.5181347150259068No Hit
GCGCCGAAGAGATCGGCGCGCAGGCTCCTTGCAGCCTTCACTTGAGTGGA10.5181347150259068No Hit
CGCGAGCGCCGCGATGTGGAAGCCGGCGTCGTGCAGCAAAGGCGCCGCCT10.5181347150259068No Hit
GTGAAATATTGAGTGGCCAGTGTCTGACCTGAGCGTTTGTGCCAAGCAAA10.5181347150259068No Hit
CTCTAGGACCCGTATGCCCAATCACGCCCGCCTCCTCACCGCCCTGCTGC10.5181347150259068No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC10.5181347150259068Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCACGTCGAGCATCTCGAGGAACGACATGTCCTCGTCGGCGTCGACCTCG10.5181347150259068No Hit
GTATCGGGAAGCCTACCACGTCAACGGGGCACAGACCTGGTGGGACACCA10.5181347150259068No Hit
CCCTTAACGTGAGTTACGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGT10.5181347150259068No Hit
GCATCGTGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTT10.5181347150259068No Hit
TCACCATGTTGGCCAGGCTGGTCTCGAACTCCCAACATCAGGAGTCCATC10.5181347150259068No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
GCTTTAAACTGAATGTTTCTAATAACACAGTCAGAAAACATACATCGTGA10.5181347150259068No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATTAAAAC10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTCTTCTTCTTTGCGCCTTCTCTCCAACTCCTCTAGACGCCTCTTTT10.5181347150259068No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
GCAGGAAAGGGACAGACAGCAGCTCCAAAGAGGCCAAGAAGTGAGAAGAA10.5181347150259068No Hit
AAGAATGACTGTAGGAGTCTCCTGATAGCAAGTAGAAAGCTGGGTGAATC10.5181347150259068No Hit
TATTCAACTTTCAAAGACTCCATTAAAGAGTGAAAGTTTTCTATTCCCAG10.5181347150259068No Hit
GATGACGCGAAAACTTTAAAGTTGTTTCTACATACAGAAAGCCCCCAACC10.5181347150259068No Hit
ACCCGGGGCCTGTGATGTGGCGCGCGTCTACAAGACGTTTCCGCGCCTTC10.5181347150259068No Hit
GTATTAAATCAGTTACAGAGGCCACCCCAGGAGACTGGAGAGAGGTTAAA10.5181347150259068No Hit
CTTGAAAACAAAGTACTTAGTTAATGCAGTCTGAAGAAAAGTTTTTTTGG10.5181347150259068No Hit
ATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAAT10.5181347150259068No Hit
GATCTGCGGCCAGGGCGAGAGGCCATGCTGGTTGCTGCAGCTGCCCAGTT10.5181347150259068No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
GATCCGGGCGCCGAACCCGTCGTCGCTCAGCGCGTCCCGGCCGCACCGGT10.5181347150259068No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
GTCAAATGCCTGTGATGGTCGAAATGCACTTTAACCTCCCAGGTTATGTT10.5181347150259068No Hit
CCATCACACATCGCGGCGCGGAAGAAACGCGGCGTCATTTCGTGGGCGAA10.5181347150259068No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAACGCAGAGA10.5181347150259068Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCAAGTACTCTGCGTTGATACCACTGCTTCCAAGTACTCTGCGTTGATA10.5181347150259068Clontech SMART CDS Primer II A (100% over 26bp)
GGGGAAGGTGCCCTGAACCTCAGACAAGAACCCCTTCCAGAAACCACCAC10.5181347150259068No Hit
GGCGACGAGCTCGTCCTGCGGCACCGCGCAGGCCTCGGCCATCCAGGAGC10.5181347150259068No Hit
GCGCTGCTGACCGAGCAGGGTGCACTGGATCGCCGTCGCCGGGTCGGCAA10.5181347150259068No Hit
TCTTTGCTTGACCAAACTTTAGCCAGGTTCCTGAACCTTCCGCTAGGCCT10.5181347150259068No Hit
GTATTTTCCATCCTTTACATCCTTCTGTCTGTTCAAGAACCAGTCTGGGA10.5181347150259068No Hit
GATGTGGGTTTTGAAAGGAAGACAGGAGTGAAGGACAACTCCAAGTTTTC10.5181347150259068No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTA10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
CGCCTCGGCCTCTCAAATTGCCGGGATTACAGGCATGAGCCACTGCACCT10.5181347150259068No Hit
GTACAGGGAAGCAGTGGTATCAACGCAGAGTACAGGGAAGCAGTGGTATC10.5181347150259068Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCT10.5181347150259068No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)