FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003620020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003620020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA94.66321243523316Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT63.1088082901554404Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG42.072538860103627Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG42.072538860103627Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT31.5544041450777202Clontech Universal Primer Mix Long (96% over 26bp)
GTGTGTGACTACGGCCGCATCAATCCAGCCCGCCCGTTTTTGCTGACCAG31.5544041450777202No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC31.5544041450777202Clontech SMART CDS Primer II A (96% over 26bp)
GTGCCGGCGTGACCAAGCATGCGCCGCACGCCGAGCAGGCCAGGAAGCTG31.5544041450777202No Hit
GCTCGCGTGGCGTCCACTCGGCCGCATCGAGGCCTGCGTCAGCGGCGACG31.5544041450777202No Hit
GAAATCATCAGAAGCTGAGGCTATAGAAAGGGAGAAGGGACAGATTGGAA21.0362694300518136No Hit
CCCGCTGCTCGGTGGCCAAGTGTCCGGCCGTGGCGTAACGCTCCCCGTTC21.0362694300518136No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21.0362694300518136Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTAGGACTGAGCCGAGTTCTCTTCAGACGATGGGAGAGAAGCCACTCA21.0362694300518136No Hit
CGATGGTGGCGCGGCTGGTGGCTGGCAGTTTGGACAGCCGCTGGTGCGTC21.0362694300518136No Hit
GTTCGGCCCGACCGCCGCAAGCTGCAAGGCCTGCCACGACAGCTTCCGCG21.0362694300518136No Hit
GCCCCTTATCGCTGGTTTCCCTTTGTGTGGTTTCAGTTACCTGCGGCCCA21.0362694300518136No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA21.0362694300518136Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGTGGGTCTTCTCAGTCCTGCCAACAGCTGTGAGTGAGCATGGACGC21.0362694300518136No Hit
CCCACTTTTCGATGGTGGAAACTCTGAACGACCTGCTGCACCGCGCTACG21.0362694300518136No Hit
GCTCTGTCGCTTAGGCTGGAGTGCAGTGGCACGATCTCGGCTCAAGTGAT21.0362694300518136No Hit
GCGCCGGTCCTGCGCAAGGAGGAGCTCGTCTTCGAGTACGTGCACCCGAC21.0362694300518136No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT21.0362694300518136Clontech Universal Primer Mix Long (100% over 23bp)
ATATTATACCGTCTGTCTAGGTCTTCCTAAAAATTGGAAAAGGCTGTAAT21.0362694300518136No Hit
GAGTCTCACCCTGTCGCCCAGGCTGGAGTGCAATGGCGCAATCTCTGCTC21.0362694300518136No Hit
CTTCTGACTAAAGGACTGCATATAGCCTGAGAAGCAACATTATGGCCCAG21.0362694300518136No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC21.0362694300518136Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGCCTTCACTTCTTCACGCACCTTGGTCTCCACGGCATCCGTCTCGCCG21.0362694300518136No Hit
GTCTGCATTGATGCCGATGTAGGTCGCTGAGGCGAACGCGACGATCGCTA21.0362694300518136No Hit
TTTTTAAAGGGACTGTGAAAGAAAAAGAAAAGAAACAAAGAAATATGCGA21.0362694300518136No Hit
CCACACCGACGTGTAGGGCCCCACGACGACCGTCGTGGCCGCGATGACCG21.0362694300518136No Hit
ATCTGCCGCTCCTGCTGCAGCAGCTCGTTCTCACGGCGCAAACCGGCCAA10.5181347150259068No Hit
TCGTAGGGCAGGGCGTAAAGCCCGACGGACATCCCTGGTGGGTCCAACTC10.5181347150259068No Hit
GCCGGGACCACCGTGGCGCCCGGTCGGAGCATCCTCCGCCGTCGAGGTGG10.5181347150259068No Hit
GGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCG10.5181347150259068No Hit
GGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.5181347150259068No Hit
GCTCTGGCCGATGCCGTGGTGCAGTACAGCCTGACCTTGCCCAACAGCCG10.5181347150259068No Hit
ACCTACGACCTCCTAGTTAGCTATGGTGCGCAAGATGAACCAGAGTTCGC10.5181347150259068No Hit
GTGCGGGATTCGTCATCAGCGCGCGAGCGATGGCGACCATCTGCTGCTCG10.5181347150259068No Hit
GGAGAGTTCACAACTGCCGCTTCAATGAGCGCACCGCCCATGAAAGCCTG10.5181347150259068No Hit
GCAATGCCCGTCGCCAGATCGATGCCGCATTGAGCCAAGCACTGGAACAC10.5181347150259068No Hit
GAGTTCTGCATTGCTGGTGAGGGATCCGCACACCAGGAAGGCCACGGAGA10.5181347150259068No Hit
CTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGG10.5181347150259068No Hit
GGTGAATTCATTGCATCCAGTGCTCGTGTGCGGTGGGGCCTTGAGCCCCC10.5181347150259068No Hit
ATCCCGCTCGACGTCCGTTACGGCGACACCGCCGAACTCGTCGCGCAGGT10.5181347150259068No Hit
CTCTACCTCTGTTACTTTTAGAGAGATGGACATGAGCCCGGAGGAAGTGG10.5181347150259068No Hit
GATCAGACATCACCTGGGGAGTGGTGGAGGATGAGGAACCTAGTCAGATA10.5181347150259068No Hit
CTGACGGTGCGCCAGGGCGAGATCCACGCCATTTGCGGCGAGAACGGCGC10.5181347150259068No Hit
GACATAGCCCAGGCCCTTGAGGCGCTCACGCAACTCTTCGTGGTTCTCGA10.5181347150259068No Hit
TTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAG10.5181347150259068No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG10.5181347150259068Clontech SMART CDS Primer II A (96% over 26bp)
CACTTGATAATCTGAAATGAGGGCCCCTGTGATCACCAGCTTCTGTCTTA10.5181347150259068No Hit
GGGCCGCAGTCGGGGCCGCTGGTGGTGGGCGTGTCGCTGGTGGTTTGTGT10.5181347150259068No Hit
ATACCACACAATCTTAATTATTGTAGCTTTATAAATCTCCCTATCTGGTA10.5181347150259068No Hit
GGCTATGGCGGGATTGTTGTTTTCCGAGCCGTCACTGCTGGACCTGACCA10.5181347150259068No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG10.5181347150259068Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAATATAATGCCAAGACAATGACAGGGGCTGGGAGAACACAGCGGAAAAA10.5181347150259068No Hit
GTTCCCGGACGCCGTCGGGATCGGGAAGCACGGCCGGGAGAACCTGCTGC10.5181347150259068No Hit
AGGGTCCCGGGTGTGTGGGTGCTGCCTGTGCCCTCACTGCTGCACAGGCT10.5181347150259068No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.5181347150259068Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTACGA10.5181347150259068No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10.5181347150259068Clontech Universal Primer Mix Long (95% over 22bp)
CAGTCTCTCCAGTCAGCCTGTCTGGGTTTAAATCCCAGCTCTGCCATTGA10.5181347150259068No Hit
GCTATTTATTATTCAGAAGGCCAGACATAAAACAAGGAGCAAGGTCGATA10.5181347150259068No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGT10.5181347150259068No Hit
CAAGCGGACGCTGAACTCCTTCACCTCCAAGGGCTGCACGGACCTCGCGG10.5181347150259068No Hit
TTTTAGTAGAGACGGGGTTTTGCCATGTTGGCCAGGCTGGTCTCAAACTC10.5181347150259068No Hit
CCCTCAGACCTGTGTCTCTTTAGCTTCTTGGTAGAGGAAGAAGAGGAGCT10.5181347150259068No Hit
GTTTTGGGACTTGGACTGGCTTTCTTGCTCCTCCCTCTTCAGCTTGCTGA10.5181347150259068No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCAGTGGTATC10.5181347150259068Clontech SMART CDS Primer II A (100% over 26bp)
GTCCTTGCCTTGCTTACCATTCTGAGTTGTTTCAAGGTCAAATTTTTTTT10.5181347150259068No Hit
GTTCTGGAAGGCTACTGAAAGGTGAACAGGAGCAGCCCTTCCTGCTTAAG10.5181347150259068No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAGAGT10.5181347150259068Clontech Universal Primer Mix Long (95% over 23bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
GTTGAGGAAAGCAGACATTGACCTCACCAAGAGGGCGGGAGAACTCACTG10.5181347150259068No Hit
ACCCACGACGCCCGCGCCGCCATCACCGGCCGGCCCGCGGGGCCGCCCCC10.5181347150259068No Hit
ACTCGGGAGGCTGCAGTGTAGGGTTGCTTGAGCCGGGAGCTGTCTCTTAT10.5181347150259068No Hit
CCTTATAGCTTAGCTCCCAAAGACATTTTTTATAAAACACTATATGGACA10.5181347150259068No Hit
ATTCTGCCCTGGGGTATGGGAATGGGGAAAAGGAAAGGGTGTGGCATAAA10.5181347150259068No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA10.5181347150259068No Hit
GTAGTGAAATAGTTACTTTGGGGTAAACTCTGAGGCACTGAGTGCTTCAT10.5181347150259068No Hit
GTATCAACGCAGAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGGG10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGACTACATG10.5181347150259068Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTGCGCCGCAATTAGCGCAGGACGCCCTGCCCCGTAGCGCGGCAGACGA10.5181347150259068No Hit
CCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGT10.5181347150259068No Hit
CAGCCCGGTAGGCCGCTACCTGCGCTACAGCGGCCAGGCGGTGTGGAGCG10.5181347150259068No Hit
GTGCGGAAGCGCCGCTGCTCCCGTGGACCTACGACTTGCGAATGCTAGGC10.5181347150259068No Hit
ACTGAGGGGATTTTAAAAATATTTTTAAATTTTGGCCAGGTGCAGTGGCT10.5181347150259068No Hit
GAGTGTCAGCGTGCAAGCCCGCCAGCGCCTGGGCGAGGAGCCTTTGGCCG10.5181347150259068No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
GAGACCATACTGGGAATCTGACAAGAATGCAAAATGAAAGAAACAAGGTA10.5181347150259068No Hit
GGGCATGCGGGATCCTCCGGCCTGCTGTTCTGCCGGGTGCTCCGTCCTGG10.5181347150259068No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.5181347150259068Clontech SMART CDS Primer II A (96% over 26bp)
CGATCAGCGCGCTGATCAGGTCGTCGGCGGGTTCGGCGCGACGCGCCGCG10.5181347150259068No Hit
GCAGCAGAGCGATGGCATTACCGGCGCAGGCGCGCGCCACTGCGCGCGGG10.5181347150259068No Hit
AAGGCGATGAACACCACCGCCGTGCCGATGCCGCGAATCATCCCGATGTC10.5181347150259068No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTAGACGGCGGGCAGGCTGCGCCGCGAGGACCAGCCGTAGGCGAGGT10.5181347150259068No Hit
TTCTTACGTAATGCTGAGCACTGCATTGGGAAGGTGGCTGAGGCCCCTGC10.5181347150259068No Hit
GTATCAACGCAGAGTACATGGGTGTACAATGACAAAGTTTGTTTAGAAGC10.5181347150259068No Hit
TTCCACATGTGCTGGAAAAGAATAAGTGTTCTTAAGTTATTCATTCAAAT10.5181347150259068No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.5181347150259068Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGTTAGGATTATCTGTTTTACAGAGAAGAGAATGAAACAAGAAAAGATG10.5181347150259068No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.5181347150259068Clontech Universal Primer Mix Long (95% over 22bp)
GATCGGTATCAAAGACAGCCGGTAGTGGCTCCCGGAGTCCCCGGTATTGA10.5181347150259068No Hit
GCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCG10.5181347150259068No Hit
CAGCATTGCACTCCAGCCTGGGCAACAAGAGCGAAACTCCATTTAAAAAA10.5181347150259068No Hit
CTGCAGCAGGTCGAGCGCGCCGACGATCGGGGTAAGCAGGTTGTTGAAGT10.5181347150259068No Hit
CTTCCAGTTCACGCACCTTGATCGCTTTCGGGTTCTCGACGATGTCCTTC10.5181347150259068No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCTGTCTC10.5181347150259068Clontech SMART CDS Primer II A (96% over 26bp)
ACGGTGTACAACGGATCACCACTGGCGTTCTGCTGAACCACTTCCGCTTG10.5181347150259068No Hit
CGCCTGCACTTCCACCGGATGAGCGAGGTCGTCGCGCTGGCCGCGAACGA10.5181347150259068No Hit
GGAGAACATGATGTCGGTCTGTTCCAGCTGGGTCGCGGCGGGCAGCACGA10.5181347150259068No Hit
GTTCGCGGCCGCGGATGCCGGGGACGCCGTGGCGATCGAGGTACGCGCGC10.5181347150259068No Hit
GATCACAGGAGTGTTGCGTGGGTTTCAAGACACTCGCACGCCCTTAGTTG10.5181347150259068No Hit
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
TTGTGTCTCTATTTCCTTCAGTTCTGCTCTGATTTTAGTTATTTCTTGCC10.5181347150259068No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTATTTTTTTCC10.5181347150259068No Hit
GTGTATGACTGTGTTTTAGGTGTCTCAAAGAAGCAGCAATTAGATTTTAC10.5181347150259068No Hit
CGGCATCTCGAGCGACGACGCCAAGAAGATCGCCAAGATCATCCGCGACG10.5181347150259068No Hit
GTTCAGGCCAGCGCTCCGGTGGATGCCACGGCGATGCGCTACGCCATTCC10.5181347150259068No Hit
GTGAATCCTTATAGGGTTTAGTTTCATTTTGATAACAATTATTCCCCACT10.5181347150259068No Hit
TCGGGACGCGGACCGCCTCGACCAGCCGGAACCCCCGGATCAGGCCGACC10.5181347150259068No Hit
GAGCATGGCACTGTGAAGGAGGCACCAGGAGCCTGTCTTCCTCGAAGGCT10.5181347150259068No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTCTGCGTTTAAACC10.5181347150259068No Hit
ATCTCGCCCCGCTGCGCCGATCCGCTTTACCGCCGCAGCGTGCGGGTCAG10.5181347150259068No Hit
ACGATGACAGGCAGGCCCAGTTCCAGGGCCTCGCCGACGACGCGCGCAAT10.5181347150259068No Hit
CCTCTGCACTTTGTGAAGATTTGACTAACTGAATCCAGAATTTTGTCTTT10.5181347150259068No Hit
CGACTGTGCTGCGTGATGTGCGTTATGTCATCGAGGCCCACTTCGAGCTG10.5181347150259068No Hit
CCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGT10.5181347150259068No Hit
AGCCCGACGGTCGCGATCGCCATACCGGCGGCGAGTGCGAGCACGAAGAC10.5181347150259068No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC10.5181347150259068Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAA10.5181347150259068Clontech Universal Primer Mix Long (95% over 21bp)
TTGATACCACTGCTTCCCATGTACGCTGCGTTGATACCCCTGCTACCCAT10.5181347150259068No Hit
AACATTAGCTTTGCTCATGCAGTCTTTCTCTTATATGCCATCGTTACTCA10.5181347150259068No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
CTCAAATGGGCTTTAATTTTTCTTTGTGTATTCATCTTAGAATGTTTACT10.5181347150259068No Hit
CATCGGGTGCATGGCCTTGAAGCCCAGGTCTTCGAACTCCACGCGCATGT10.5181347150259068No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGG10.5181347150259068Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTGAAGGAATGAGTGAGCAACGTCAACACCACGATGGTACGCTTTAT10.5181347150259068No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)