FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003620035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003620035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA221.7001545595054095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT191.4683153013910355Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG161.2364760432766615Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG120.9273570324574961Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT90.6955177743431221Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC80.6182380216383307Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG60.46367851622874806Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT50.38639876352395675Clontech Universal Primer Mix Long (100% over 23bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC50.38639876352395675Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT40.3091190108191654Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC40.3091190108191654Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC40.3091190108191654Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGCCCATGGCCTTCAGCACGGGAGCCAGCGCATCGACACCGATCCCGGC40.3091190108191654No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC40.3091190108191654Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG40.3091190108191654Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT40.3091190108191654Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC40.3091190108191654Clontech Universal Primer Mix Long (96% over 26bp)
CATGAAGAGAGACAACTGGGAGACTGCCAAAGGCAAGTGTCTATTAGAAT40.3091190108191654No Hit
CCCCAGCGATGAGCCCACAGTTCCGGTCACAGCCCATTTTTCTTGTTCAG40.3091190108191654No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC30.23183925811437403No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA30.23183925811437403No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG30.23183925811437403Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC30.23183925811437403Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT30.23183925811437403Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATTAAATGTCACCACAAAGTCATTGGCTCCTTCCCGATAGATGACACT30.23183925811437403No Hit
TCAACGCAGAGTACATGGGGGGAGGGGCTCTGCGTTCTGTAGTGGCGCTG30.23183925811437403No Hit
GATATCATCAAACGCCAAGGACTGCAAGCTGAGGAGAATGCAGTGAATGC30.23183925811437403No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT30.23183925811437403Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAATGGAACGGAATGGAATGGGATGGGATGGAATGGAATGGAATGCAAGT30.23183925811437403No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA30.23183925811437403Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA30.23183925811437403Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCAGCTTGACCAACTCACCAACCGGCAACGTATCTAGATTGAATGAC30.23183925811437403No Hit
ATTGAGCACACTGGTTTGTGCTTTGCCGTTTTTATCATAAAACAGTGCGC30.23183925811437403No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.23183925811437403No Hit
GCTATTTAGTTTCTATGATTCAAGTATCAAGAAGGAACAACTAGAAGAAA30.23183925811437403No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30.23183925811437403Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGCT20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATAC20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
ACTTCTGTGTCTCCCGGTCCCAATACTCCGGCCCCTCCTGCTCCACCCAC20.1545595054095827No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA20.1545595054095827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCT20.1545595054095827No Hit
ACAAAAACCCCGATGAGCCGCCGCCTGCATTTGAGTCCAACCTTAGTGTC20.1545595054095827No Hit
AGCCATTGAAGCCTTAGCCGGTGATGCGGAAGCTGAAGGCTACGCCGACA20.1545595054095827No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA20.1545595054095827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.1545595054095827Clontech Universal Primer Mix Long (95% over 21bp)
CCGATATTGAGTGCTGCCCATACGCCTTTATCAGTGACTTCATGCTCAAT20.1545595054095827No Hit
GTCTAATATCCCCAAACCTGAGATGAGCACTACGATGGCAGAGAGCAGCC20.1545595054095827No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGTACC20.1545595054095827Illumina Single End PCR Primer 1 (100% over 33bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAGAGTACATGG20.1545595054095827Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
CACCACCATCAATCATTTCATTGCTGATGTTGTCGTTCAATAACGCAAAT20.1545595054095827No Hit
ACGCAGAGTACATGGGTGATTGCAGCTGCCCAGGAGGCTGAGGCTGGAGA20.1545595054095827No Hit
ACAAGAACCAGTGAAGAAATCCATTCAGGAATCAGAAGCTTTTTTGCCTC20.1545595054095827No Hit
GTGTACACCTGGTCTTTACTCCATCGGCAGGGCCCGAGCCTGTCTCTTAT20.1545595054095827No Hit
GTTTTAATTTCTTATCTTGGTGAAATTAAAACAACGCTGTAACTCTTGAG20.1545595054095827No Hit
ATGTTCATAATAAAAGGTTAAAAAATAAAATAAAAAAGTGAAAAAAGAGG20.1545595054095827No Hit
GGCAAAACCATCACCCTTGAGGTCGAGCCCAGTGACACCATTGAGAATGT20.1545595054095827No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
GGTGACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAA20.1545595054095827No Hit
CTCAGGAACTCTCCTTCTTTGGGCCACGGAATTAACCCGAGCAGGCATGG20.1545595054095827No Hit
GTATAAATCCGCAGGATTGGCCAAGTCAGGACGATTGGCAAGCACGGTAA20.1545595054095827No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGC20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
AGTCAGGAGAATCGCCTGAACTGGGAGGCAAAGGCTGTGGTGAGCTGAGA20.1545595054095827No Hit
TACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAGTA20.1545595054095827Clontech Universal Primer Mix Long (95% over 22bp)
GATTGGCTTTTTGCTGATTATGATTTAGTCAATGAAGTGGAGGCGAGAAA20.1545595054095827No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20.1545595054095827Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAGCAATAAGGAGTCTCTGGATCAGATCATTGTGGCCACCCCAGGCCTCA20.1545595054095827No Hit
GTGTGTAAGGAGTATACCATGAGATGAGATGACCACCAATCATTTCCTTG20.1545595054095827No Hit
AAACAACAATGTGCAATCAAAGTCCTCGGCCACATTGTGAACTTTGGGGG20.1545595054095827No Hit
ATCCCACCTATGCCAATTTATCCTTTCGAAAAAAGACACCCGGGCCAGGC20.1545595054095827No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC20.1545595054095827No Hit
GTCCGGACTGGTCGAGATGGCAGTGACCGTGGGAATGAAGTTGGCACTGG20.1545595054095827No Hit
GTATCAACGCAGAGTACATGGGCAGGGAGGGCCCCTGGCCGGAAGACGCC20.1545595054095827No Hit
GTACATGGCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAG20.1545595054095827Illumina Single End PCR Primer 1 (97% over 36bp)
GATCCACCCGCCTTGGCCTCCCAAAGTGCTGGGATGACAGGCATGAGCCT20.1545595054095827No Hit
CTTCCTCCCCTTGCTAAAGCAAGGGGATTCCTTCTACAAGACGGTCAAGC20.1545595054095827No Hit
GGCTGGAGTGTAGTGGTGCGACCTCAGCTCACTGAAACCTCTGCCTCCCG20.1545595054095827No Hit
CCAGTGGCCACAGAAAGTCGTCCCAGGCCCCGATGAACGCGAACACCGCG20.1545595054095827No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
ACCTCCATGTGTCCTAAGGTGGGGTCAGCAGACGACTCGAAAAAGAGCTG20.1545595054095827No Hit
ACGTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTT20.1545595054095827No Hit
AATAATTTGTTTTTCTTAGTCATTTTTACATTTTAATTCATCATAGATAT20.1545595054095827No Hit
AGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT20.1545595054095827No Hit
GTCGTACACTTTGCACTGCATGTGCCCGGTGCTCTGCACCACGCACGACA20.1545595054095827No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC20.1545595054095827Clontech Universal Primer Mix Long (96% over 25bp)
GCTAAAAGGGCAAGGCTTTGTGCGGCTGCGGGTCGATGGCGAAATTTATG20.1545595054095827No Hit
GGCTTTCACAGTTCCATAAAAGCCGATTTTTTCGCTGAGGACTTGAACCG20.1545595054095827No Hit
TTTGAAAGCCTGTTTCACCGACTGCGTGACGCGATTGTTGAGAAAAACCT20.1545595054095827No Hit
CTGGCCATCAGAGAAATGCAAATTGAAACCACAAGGAGATACCATCTCAC20.1545595054095827No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT20.1545595054095827Clontech SMART CDS Primer II A (96% over 26bp)
ATTTGCGGTAACTGACTTATCTCAATTAACATAACCCCGCCCCCTTTTTT20.1545595054095827No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA20.1545595054095827No Hit
GTGCCGGAGTCGGTGGACCTCGTGGCGGTGCACGACGCGGTCCGGCCGCT20.1545595054095827No Hit
GCCTATTACCACGGCTCGGGCGACGAACCGCATCAGCGAGCCAAGTTTCA20.1545595054095827No Hit
GTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCGA20.1545595054095827No Hit
CTGTATTTGTTTCTGCTATCACAGTCCAGAGTCAAGCTTCATTTATAAAG20.1545595054095827No Hit
GTGGTGACGCGCGGATCATTGGTGGTGGCCAACATGGGACCGGATTCTTG20.1545595054095827No Hit
CACCACGAGTCTACCTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTG20.1545595054095827Illumina Single End PCR Primer 1 (96% over 25bp)
AGATGAAGGGATCCACGCAGCTGTTGAGGGTGCTCAGCGCCAGGCTGGGC20.1545595054095827No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
GAAGAATGGAGAGAGAATTGAAAAAGTGGAGCATTCAGACTTGTCTTTCA20.1545595054095827No Hit
GGGCTATTCCTTTCCTCCAATGAACCAAACGCCTCCCGCCCACCTCCAGG20.1545595054095827No Hit
GTGTCTGAACTCACCGCCATCGCTCCCTTAACCAAAGATGGGGCAGCTCG20.1545595054095827No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
GATCTACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTA20.1545595054095827Illumina Single End PCR Primer 1 (96% over 33bp)
GGTTAGAAGTGTCAGTTCAGGGAACCGGAAATTGCTTGGCTCAGCTGACA20.1545595054095827No Hit
GGGCGGAACAAGGGGTAGCGGGTCTCCAGGACCTGGCATTAGGACGTGCG20.1545595054095827No Hit
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTT20.1545595054095827Illumina Single End PCR Primer 1 (100% over 38bp)
GTGGAATCTTTCTCAGTCGCCCAGATTGGAGTGCAATGGCGTATTCTTGG20.1545595054095827No Hit
TGCTAGAGCAATTGCCGCCTAAAATGACGGCGCTAAACACCCTGCAACAG20.1545595054095827No Hit
GTCCATGAAATTACGCAAGACAACGAAACCAAAGAATATCGCTCAAATAT20.1545595054095827No Hit
GGGACAGAGTGTAGATCTCGGTGGTCGCCGTTCACCCATGTACTCTGCGT20.1545595054095827Illumina Single End PCR Primer 1 (96% over 31bp)
CCTGGTCTTTTTTCCGCCGAATGATCGCCATATGCGGAATGGGCAAATGC20.1545595054095827No Hit
GTCCAATGTCCGATCCATCAGGAAGTCAAACAGTAATTAAATTAGCAACA20.1545595054095827No Hit
AGTTTAATTAGCCCCTGGGGCTCTACAGGGAAGAAATAATCAGACTTCAA20.1545595054095827No Hit
CATTGGTTCCTAGGCATTGCTTCACACTCACAGATAACCTGTTGGACCTT20.1545595054095827No Hit
GATCGGAAGAGTGTCGTGTAGGGAAAGAGTTAGATCTCGTGGCTGTCTCT20.1545595054095827Illumina Single End PCR Primer 1 (96% over 30bp)
TTATAAAATAGGCCCAAGGGAACTTGTTCTCCCCTTCCACCATGTGAGAA20.1545595054095827No Hit
GATCGGAAGAGCGTCGTGTAGAAAGGTAGATCTCGGTGTCGCTACTCCCA20.1545595054095827Illumina Single End PCR Primer 1 (95% over 21bp)
ACTTTTATAAGAAAAGGAGCACACAGAAGGCAGAAAGGGACCTTGTTTGG20.1545595054095827No Hit
GTGTTTCCACAGCTTCTCTACTCCACTGTCACCCCTCAGGCGGGTCCAAG20.1545595054095827No Hit
CTTGTTGCCCAGGATGGAGTGCAATGGCGAGATCTCAGCTCACCACAGCC20.1545595054095827No Hit
TATTGTAAAAGGAAGACGTTATAGATAGGTAAATAACATGCTCAAGATTA20.1545595054095827No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAAC20.1545595054095827No Hit
TATCAACGCAGAGTACATGGGACTGCGCATTAGGGCAGCGTCTGAGGAAG20.1545595054095827No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTACCATGTA20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)