FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003620036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003620036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA322.472952086553323Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT191.4683153013910355Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG161.2364760432766615Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC100.7727975270479135Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT90.6955177743431221Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC80.6182380216383307Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG70.5409582689335394Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC70.5409582689335394Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG60.46367851622874806Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT50.38639876352395675Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAGTCTTGAGGTCCTACATCTCCGAAAGC40.3091190108191654No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC40.3091190108191654Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC40.3091190108191654Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA40.3091190108191654Illumina Single End PCR Primer 1 (100% over 21bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG40.3091190108191654Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT40.3091190108191654Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA40.3091190108191654Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT40.3091190108191654Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC40.3091190108191654Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA40.3091190108191654Clontech Universal Primer Mix Long (96% over 26bp)
GACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCG30.23183925811437403No Hit
CTTTACTAGTATTTGCATCAATAAATTCTTTTTGATAGACAGCGATTTTT30.23183925811437403No Hit
CAATATATGTGTGAAGGTTGGAGAAAAGAGTCTAACCTGCAAAAAAATAG30.23183925811437403No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.23183925811437403Clontech Universal Primer Mix Long (100% over 23bp)
TTTGAGGAAGTTGTCAACATCTTGGTCGGAGATTTTGATGCGGCTGGCAA30.23183925811437403No Hit
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG30.23183925811437403No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT30.23183925811437403Clontech Universal Primer Mix Long (96% over 26bp)
AACCAAACTTTAACCTTGGTGGATTTGATTACCCAAATCCAAACGCCAGC30.23183925811437403No Hit
CGGTCGATGTGCAGAACTTGCGGCGCGCGCTGTCCGCTGTGTTGGAAGTA30.23183925811437403No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA30.23183925811437403Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTG30.23183925811437403No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.23183925811437403Clontech Universal Primer Mix Long (96% over 26bp)
ATTCCATTCCGTTCCATTCCATTCCACTCGTGTTGATGCCATTATGTTCC30.23183925811437403No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA30.23183925811437403Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.23183925811437403No Hit
TATCACTACGGTGCCTGGCGCGACCATGCCAAGGATCTCTTCGAGAACGA30.23183925811437403No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC30.23183925811437403Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAACGGCGACACAGATCTACACTC20.1545595054095827Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GGTAAAGTATAGCAAGGTTTGAGAAATTTATAAAAAATAAAAAAAACGCC20.1545595054095827No Hit
TGGTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGA20.1545595054095827No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.1545595054095827Clontech Universal Primer Mix Long (95% over 22bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1545595054095827Clontech SMART CDS Primer II A (100% over 21bp)
GGTCTTTATTTGCTCTCTCAACTTCCAGGAATTGACTTATTTAATTAATC20.1545595054095827No Hit
CTCAAAGTCCTGACCTCAAGTGATCCACCCGCCTTGACCTCCCAAACTGC20.1545595054095827No Hit
CCCCTAGAGGCCAGCTGTTTACCAAACCCGAAAGAAATCCTATCTGCCTG20.1545595054095827No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT20.1545595054095827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTCTGTCTCTTATAC20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
CTGCCCACCTTGGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCTACCG20.1545595054095827No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA20.1545595054095827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.1545595054095827Clontech Universal Primer Mix Long (95% over 21bp)
TTTATATACTGAATGGACGTAGCTTCACCTGTAGGCAATTTTTGAAATTT20.1545595054095827No Hit
GGAGAAATGAGTGAGCGCTGCGGCTCCCGGCCAGGGGCCCTCCCTGCCGC20.1545595054095827No Hit
CAGCAGTGGGTGGCAGTTGAGAAAGAGTGGTTGTCATGGTATTGCCCGAT20.1545595054095827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20.1545595054095827No Hit
GCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGCGTCATGGATTTCA20.1545595054095827No Hit
GGTGAAACCCTGTCTCTACTAAAAATACCACAAATTAGCTGGGCGTAGTG20.1545595054095827No Hit
GCTCATGCCTGTCATCCCAGCACTTTGGGAGGCCAAGGCGGGTGGATCCT20.1545595054095827No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20.1545595054095827Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACAAGGGAAGCAGTGGTATCAACGCAGAGTAC20.1545595054095827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAA20.1545595054095827Clontech Universal Primer Mix Long (95% over 21bp)
CCACGAGATCTAACTCTTTCCCTACACGACACTCTTCCGATCCTGTCTCT20.1545595054095827Illumina Single End Sequencing Primer (96% over 30bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA20.1545595054095827Clontech Universal Primer Mix Long (95% over 21bp)
GTCGTCACCTCCATCCGCCCCATCGTCGGAAACCCTTCCGCCTGTCAGCG20.1545595054095827No Hit
CAAGTACTCCGTGTGGATCGGCGGCTCCATCCTGGCCTCGCTGTCCACCT20.1545595054095827No Hit
GATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTAGAATA20.1545595054095827No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA20.1545595054095827No Hit
GTGTAAGGCAGTAAGCCAACTTGGATGTCTAAGGCACGAACCGTTTGCAA20.1545595054095827No Hit
TCAATGTGGTGATAATATTGATGAAAAAGCGGGTTGCGAATAATGGTCAA20.1545595054095827No Hit
CAGATAGAATGTCCAGAAATCTGTGAATAACTTTATGAGATACAGACATT20.1545595054095827No Hit
GTACATGGGTGATACGGCGACCACCGAGATCTACACTCTGTCCCAAGTTC20.1545595054095827Illumina Single End PCR Primer 1 (97% over 35bp)
TTATTGAGGCTCCAGGGCCACGGGGCCTGGGCAGGAGGCTGCCCTTCAAG20.1545595054095827No Hit
CCCCTGTTGCTTTTGCCAGTTGAATAGGGACAAGTAAATCACTCGAAGCA20.1545595054095827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1545595054095827No Hit
TCCGTGTCCCGGCCTGGCCGCGGAGAGCCCCGCTTCATCTCAGTGGGCTA20.1545595054095827No Hit
GAGATACACGACCCAGCGATGTTCGTGTTGCGGTGAAATCACCGCCAATA20.1545595054095827No Hit
GGCCATTCCGGGTGTCGTCGGTGCCATCAACGTTCTGCTCATGGCGACCG20.1545595054095827No Hit
GGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTC20.1545595054095827No Hit
GTGTTGTGTACCTATAGTCCCAGCTACTCGGGAGGCTGAAGTGTGAGGAG20.1545595054095827No Hit
GCCACAACTCCTCCAATCACAACTGTAGCAATCCTGGCCAGGGGCAAAAC20.1545595054095827No Hit
CGGCTGATTTGGGCGATGAGTTTTATATCCATGTCAATGCCAATAACTTG20.1545595054095827No Hit
ATTTTTAGAAGTCCCGGATTTTTGTTTTCTCAGAACAATGATGAGTCCTC20.1545595054095827No Hit
TAGATAGACAGTGCCCAGTGGGCAGGTGGCCAGCAAAATGCAAAGGAGAG20.1545595054095827No Hit
GAATATAAATATCACAAAGTTGTTTAAAAAAAAAAAAAAAAAAAAAAAAA20.1545595054095827No Hit
GTCTGGGCCAGCCAGAGGTTCGCCCGCGGGACGGTTCCCGTTATCCGGTT20.1545595054095827No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACT20.1545595054095827Clontech SMART CDS Primer II A (100% over 26bp)
ATCCAAATGCGGCATCTTCAAACCTCCATGATGCTGCTTACATGTCTCGA20.1545595054095827No Hit
GTCGCCCAGATTGGTGTATTAGCCGCCAACGTACTTGGGGCGTGCCGATT20.1545595054095827No Hit
CCAGATCGTCGTCTGGGGAGGCTGAACGCTTCACCCCATGCGGGTGAGAC20.1545595054095827No Hit
AGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAAC20.1545595054095827No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTA20.1545595054095827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGGGACGCGCGGCGGTCTGCGGGGAATGACGAGGCCGACCTCGTCTGC20.1545595054095827No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
CCACGATGATGTTCTCGGGCTTCAACGCAGACTACGAGGCGTCATCCTCC20.1545595054095827No Hit
GCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAA20.1545595054095827No Hit
CGTATAGGATGAATGTGGGCTATAACACCTTCCTTATCTCCCTAACGGTT20.1545595054095827No Hit
GTCATCGGTTGGGGGTTGACTGCACCCATTTGGTAATCACTCAGCGCTTT20.1545595054095827No Hit
GTGTCATTGTCTAAAAACACCAACCGCAAATCATGCAAGGTGCCTACATT20.1545595054095827No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTACATGCCTGTAATCCCAGCTACTCAGGAGGTTGAGTCAGGAGAAT20.1545595054095827No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC20.1545595054095827Clontech Universal Primer Mix Long (96% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.1545595054095827Clontech SMARTer II A Oligonucleotide (100% over 24bp)
ACATTCTCAGCTTAACACGCGCGCTAAAGTTGTCGCCACCCTTGAGCGTA20.1545595054095827No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC20.1545595054095827No Hit
TTGTACTCCCTGCTGTTCCGCAGGACCTCCACCTTCGCCCTCACCATCAT20.1545595054095827No Hit
GCTTTCTAGTTCGTAGACAGTACCTTGTCATTATGTTCTCACATGGTGGA20.1545595054095827No Hit
ATCATAAACATGCTATCATTAAACCGGCTCAAATAGCCATTTTTCACCCG20.1545595054095827No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA20.1545595054095827Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT20.1545595054095827No Hit
GCCCAGGGAAGTGAAGGTGTCTCGAAGAAGCTCTGTCTCATTGCCAATGC20.1545595054095827No Hit
AGCTCACACATGGCCCCCAGTCCCCTCCTTCCTCTCAAGAGTTACAGCGT20.1545595054095827No Hit
CTCTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGG20.1545595054095827Illumina Single End PCR Primer 1 (100% over 23bp)
CCAGACGACCTTCTCATCGCAGGTCGGTGTGGTGAGTGAGCCCAGGTAGC20.1545595054095827No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT20.1545595054095827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAGAGGCGCGCACCGCCACGCCCGGCTAATTTTTGTATTTTTAGTAGAG20.1545595054095827No Hit
ACATTCTCCTCATCTTTCATATTTTCTTTGGCTCGCTCGGCTAACATCTC20.1545595054095827No Hit
ACACAAAGAGGGATCCACCCTGGCCTTCCTGGGTCATGAGAGGCCTGCTT20.1545595054095827No Hit
CTCAGCGTCCATATCATGCAAGATAGCAATGTCGCTACCCAGTCGTAATG20.1545595054095827No Hit
CGCTGAACCAATTTGCGTTATTGAACGACAACATCAGCAATGAAATGATT20.1545595054095827No Hit
GGTAACTGCGTGGTGATTGTGGTGCGTTACTTTGGCGGTATCAAACTTGG20.1545595054095827No Hit
GAGTCCACCTTTCAACTCTTCCAAATGACACTTGACTTGTCACTCCCCAG20.1545595054095827No Hit
GCTCGGGCCCTGCCGATGGAGTAAAGACCAGGTGTACACCTGTCTCTTAT20.1545595054095827No Hit
ATCAACGCAGAGTACATGGGTTGCAAAAAAAAAAAAAAAAAAGAATAAAC20.1545595054095827No Hit
CTTTCAAATACCAGGAATGGACTCTGGGGAATGGGGCACCCTTGAAGTCT20.1545595054095827No Hit
TCACCACACTGACTTCCACAATGGTAGAACTAGTTTACAGTCCCACCAAC20.1545595054095827No Hit
GTATGTTGGCTTGGATTTTGGGCGAGTGATGAAGTTTAATCCCAAAGATG20.1545595054095827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1545595054095827No Hit
GCCTTGGGGGTTCTGCGGGCCTTCGGGTGCGCGTCTCGCCTCTAGCCATG20.1545595054095827No Hit
CCACCAGGAGTGGAGCCTGCGGCTTAATTTGACACAACACGGGAAACCTC20.1545595054095827No Hit
GGCGGGAGCTGGGAGTACTGCTAGAGGGTGTGGACCTGGTGTTTGGGTGA20.1545595054095827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)