FastQCFastQC Report
Tue 10 May 2022
EGAF00003620037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003620037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576396
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT13030.22605986162291203No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA11920.2068022678852733No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT9720.16863406408094433No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG9090.15770407844606832No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT8720.15128488053352207No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT8340.14469219078550163No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8190.1420898132533883No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA8050.1396609275567492No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC7750.1344561724925225No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC7680.13324172964420294No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA7640.13254776230230605No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG7600.13185379496040917No Hit
CCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTC7560.13115982761851228No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC7560.13115982761851228No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA7350.1275164990735536No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA7200.12491412154144026No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7060.12248523584480116Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTT7040.1221382521738527No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC7040.1221382521738527No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT6980.12109730116100735No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT6960.12075031749005892No Hit
CCCCATACCCAACCCCCTGGTCAACCTCAACCTAGGCCTCCTATTTATTC6910.1198828583126878No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT6910.1198828583126878No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC6880.11936238280626514No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA6870.11918889097079091No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG6820.11832143179341979No Hit
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA6750.11710698894510024No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC6720.11658651343867757No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT6590.11433111957751267No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT6580.11415762774203847No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6570.11398413590656424No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC6550.11363715223561578No Hit
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT6470.112249217551822No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG6320.10964684001970867No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG6310.10947334818423446No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA6220.10791192166496645No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT5970.10357462577811088No Hit
GTCTACTGAGTAGCCTCCTCAGATTCATTGAACTAGGTCTGTCCCAATGT5940.10305415027168821No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC5830.10114574008147177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)