FastQCFastQC Report
Tue 10 May 2022
EGAF00003620038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003620038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576396
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT13580.23560191257399427No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA11420.1981276761115622No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG9430.1636028008521919No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT9260.16065343964913012No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT8680.1505909131916252No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG8540.14816202749498608No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC8420.1460801254692954No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT8330.1445186989500274No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT8260.14330425610170786No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7930.13757902553105852No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC7770.13480315616347094No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA7690.13341522147967716No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC7680.13324172964420294No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA7580.13150681128946073No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT7480.1297718929347185No Hit
CCCCATACCCAACCCCCTGGTCAACCTCAACCTAGGCCTCCTATTTATTC7460.12942490926377004No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA7280.12630205622523405No Hit
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA7210.12508761337691449No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA7170.1243936460350176No Hit
CCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTC7150.12404666236406915No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT7130.12369967869312071No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC6980.12109730116100735No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6760.11728048078057447No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC6590.11433111957751267No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC6590.11433111957751267No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT6510.11294318489371889No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG6450.11190223388087357No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA6420.11138175837445088No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6400.11103477470350245Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT6380.11068779103255401No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT6320.10964684001970867No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT6310.10947334818423446No Hit
TCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTT6250.10843239717138911No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG6110.10600351147474998No Hit
GGTGAGAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCC6000.10409510128453354No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA5950.10322764210716244No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC5890.1021866910943171No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC5840.101319231916946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)