FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003620058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003620058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length140
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAATTACAGTACCATTGCAGGCAAACTTTTTCTTAAACGCCTTCACTA216.666666666666664No Hit
TGCCCGGCTTCCGCGAGGTGCTCGACGACCGGCAGATCGCCGAACTCGCA216.666666666666664No Hit
GGAGCAGCGTTGGTGTTGCTCAGGAGCTGGTGATAGAAGCCGATCATCTC18.333333333333332No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA18.333333333333332No Hit
GTGCCTCGGTGATGCGCACGCGGGGGTCGTACTTCAGGTAGACGCCGAGG18.333333333333332No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGATTTATATTATAA18.333333333333332No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA18.333333333333332Clontech Universal Primer Mix Long (96% over 26bp)
CTCCGAGGACGATCCGAACGCCGGCGCCGTCGTCTGACGGATACTCCGGG18.333333333333332No Hit
GAGTGTGCGCATGAGAGCCACTGGGACCCTCGATGACGAGCCCTATCACC18.333333333333332No Hit
GGTGGGCTCTTCCTTCTCAATGTTCGGTTTCAGGCTGCCGCGGTCAGCGG18.333333333333332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)