FastQCFastQC Report
Tue 10 May 2022
EGAF00003620102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003620102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100004
Sequences flagged as poor quality0
Sequence length146
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG8220.8219671213151474No Hit
CTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCA5580.5579776808927642No Hit
CTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGC5310.530978760849566No Hit
GTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGT4420.4419823207071717No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG4360.4359825606975721No Hit
GGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGC3960.39598416063357467No Hit
GTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGT3360.3359865605375785No Hit
GCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCC2860.2859885604575817No Hit
CTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTT2850.2849886004559818No Hit
TTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGA2820.28198872045118195No Hit
GCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCC2740.27398904043838246No Hit
GTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTG2710.2709891604335826No Hit
GTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGG2640.2639894404223831No Hit
GGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGG2440.2439902403903844No Hit
TGCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTGAGGGTGCCGAGGGGG2370.23699052037918483No Hit
GCCTGGGGACCCAGCTCAGCCACACACTTCTGGGAGCCCTCTATGAGGTG2300.22999080036798528No Hit
CTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCC2300.22999080036798528No Hit
GCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCC2290.22899084036638534No Hit
CTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTC2240.22399104035838568No Hit
GGGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGG2190.21899124035038597No Hit
GGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGA2150.21499140034398623No Hit
GCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGACCCTGGCCAACCCCCT2090.20899164033438664No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG2040.20399184032638693No Hit
GCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGACCCTGG2040.20399184032638693No Hit
GTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCT2000.19999200031998718No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG1980.19799208031678733No Hit
GGGGAAGGGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCG1970.19699212031518742No Hit
GTACATGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATGA1970.19699212031518742No Hit
CCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAG1930.19299228030878765No Hit
GCCTGGGCATCCCCGTGAACCACCTCATAGAGGGCTCCCAGAAGTGTGTG1910.19099236030558778No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG1880.18799248030078797No Hit
ACCTCATAGAGGGCTCCCAGAAGTGTGTGGCTGAGCTGGGTCCCCAGGCC1850.18499260029598816No Hit
CACTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCT1780.1779928802847886No Hit
CCCTCAACCCGCTGAAGCTCCTGCTGAGCAGCCTGGGCATCCCCGTGAAC1780.1779928802847886No Hit
CCCCGAGCCCCCGCGCCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTG1720.171993120275189No Hit
TCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTA1690.16899324027038917No Hit
GCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGG1690.16899324027038917No Hit
ACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC1680.16799328026878926No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC1660.16599336026558936No Hit
CCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGC1550.1549938002479901No Hit
CTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCAGCTCAGCCACAC1530.15299388024479021No Hit
GCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACC1490.14899404023839047No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT1480.14799408023679053No Hit
CCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTT1450.14499420023199072No Hit
GTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTGAGGG1430.14299428022879085No Hit
GCTTTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCT1400.13999440022399104No Hit
TCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAG1370.13699452021919123No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACAGGC1350.13499460021599136No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT1350.13499460021599136No Hit
ACCCTGGCCAACCCCCTCGGCACCCTCAACCCGCTGAAGCTCCTGCTGAG1330.13299468021279148No Hit
CCCACGGCCTGGGGACCCAGCTCAGCCACACACTTCTGGGAGCCCTCTAT1320.13199472021119155No Hit
GGCCAACCCCCTCGGCACCCTCAACCCGCTGAAGCTCCTGCTGAGCAGCC1310.1309947602095916No Hit
TCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGC1270.1269949202031919No Hit
GTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGC1270.1269949202031919No Hit
GGCCGGGACCCTGGCCAACCCCCTCGGCACCCTCAACCCGCTGAAGCTCC1240.12399504019839207No Hit
GGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGT1220.1219951201951922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.1219951201951922No Hit
CCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCA1200.11999520019199232No Hit
GTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGC1200.11999520019199232No Hit
CGGGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCA1170.1169953201871925No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC1160.11599536018559257No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA1140.11399544018239272No Hit
GGCCGGGGCCGGGACCCTGGCCAACCCCCTCGGCACCCTCAACCCGCTGA1130.11299548018079277No Hit
CCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGC1120.11199552017919284No Hit
TCCCAGAAGTGTGTGGCTGAGCTGGGTCCCCAGGCCGTGGGGGCCGTGAA1110.11099556017759289No Hit
CTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGG1090.10899564017439302No Hit
GCCACACACTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAG1080.1079956801727931No Hit
AGCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGG1080.1079956801727931No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG1070.10699572017119315No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA1070.10699572017119315No Hit
CCTCATAACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGA1050.10499580016799329No Hit
CGTTTATTGAGAGGGGCCGGGGGAAGGGGATGGACGGTCCTCCCCGCGGC1040.10399584016639334No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA1030.10299588016479341No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)