FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003620178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003620178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86
Sequences flagged as poor quality0
Sequence length100
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCCTAGTGTCTGTAGCTACTACAGACTCAAGGGAAATTTGATGATGG44.651162790697675No Hit
GCCGGCCCCAGCAGGAAGTGCACGAACTGCGCGCCGTCAAAGTAGGTCTT33.488372093023256No Hit
GTCCATACCTTTCTCGGCGTGCTGTACGCCGCCGCCAGGCGACCGGACAT33.488372093023256No Hit
GCAATGGCTTGTACCAGCGCAGAATCGCCGCAATAAAGGCCGGCGAATGC33.488372093023256No Hit
GAAGAGGGGCGGGGCGGCGAGGCGGCCCGGCGCGATGCCGAGCTTGGCCA22.3255813953488373No Hit
GTTTCGAAAACTTCGAGAAATGGGAGGGCACCCAGAACTTCACGGTGCGC22.3255813953488373No Hit
GCATTTCCAACCGCGCCTTTGCCTGCTGATGGAGACCGAGATCTGGCCGG22.3255813953488373No Hit
GCGATAAAGGCTGGATTGGCGTGTTACTGGAGATCTGGCTAGGTGCCAGC22.3255813953488373No Hit
GTCATGGTCAAACATGTAGAGCGCCATGCCGTGTTGATCCGTCAGCGTGC22.3255813953488373No Hit
TATTCGTACAGGCCCTGCTGCGCCTTGGTGGTCGACTTCACGTGCCACTG22.3255813953488373No Hit
GTCCACCGCCGGCCGGCGACCTGGTCGGCGGTCACCGGCCGGCGGTGGAC22.3255813953488373No Hit
GATCGAACACACACACATCGGCCATCGCACCCACTTCCAGCGAGCCCGCA22.3255813953488373No Hit
GAACAGGCCGATGCCGGTCTCGGGCATCGCGAGCTTGGAGCGCTCGCTCA22.3255813953488373No Hit
GACCAGGATCACGGTCTGCTCGGTCAGTGATTCGATCGTGGACCACACCC22.3255813953488373No Hit
TATCAGCTCCGGATGAAAGATCCTCTCTGAGCGCTCTGCAGACTTCGATT22.3255813953488373No Hit
TTGTTTCCGCAGTCGGTGGCGGATGAGCTCGTCGGAGACTATGCATGAGA22.3255813953488373No Hit
ACGTGGATCCGCACACGTGGGAGGTGTTGGCCGCCATCGAGCGACGGCAG22.3255813953488373No Hit
GTACGCAGGTCGAGGAATACCGCTTCCAGAAGGGCCAGGCGCCGGACATC22.3255813953488373No Hit
TTTCCGTCGACGCTCGCGGTACCGACGACACAGGGGTTAAACATTCTTAC22.3255813953488373No Hit
CTTTTTGGTGGAAAATATTTCCCCTTTCCGAAATCGGTTGAGCTTGTCAG22.3255813953488373No Hit
CTACTTACTTGCCGCGGGAGATCATGGGGCAGGACCCTAATAGCAGTAGT22.3255813953488373No Hit
GGCGCCGGCGCAGCGCGGCCAGCAGCGTCGTGCCCTGCGGATAGTCGGCA22.3255813953488373No Hit
GGTCGAGCCGGTGGCCGACCTGCGCAATGACGGCAAAGACACGCTGACCG22.3255813953488373No Hit
GGCGTGGTCGCCGAACGGCGCCGGCAGCAGGCCCGGCATCAGCACGAAAC11.1627906976744187No Hit
AGGCCAGGCCGATGACCGGCACGTAGATCAGCTGTTGCTTGAGGAAGAAC11.1627906976744187No Hit
CTTGTAGACATGCGCCGTCTTGCGCATCGGCGCATAGGCAGGCCAGCGGC11.1627906976744187No Hit
AACCCATCGATCAAAGAACGCCCTCCCACGGCCAGCCCAGTTCGGTAACG11.1627906976744187No Hit
TCATCGCCACGACCACGGTCGGCACGACGCTTGCAGACTTCGCGGATCGC11.1627906976744187No Hit
ATTCCACGTTGAACATTTCGCGCGATTGCGCACCGATGTCGCGCAGCCCG11.1627906976744187No Hit
GCGCTTCTGCGACGTCTCGGCCCTTGCCGTGATCACGAGCCTCGGACGCG11.1627906976744187No Hit
TATTCGTACAGGCCCTGCTGCGCCTTGGTGGTCGACTTCACGTGACACTG11.1627906976744187No Hit
ACAAGGAAGGCCATTGACATGCTCATCCGCCTGACTCCGCTCGCCGCGGC11.1627906976744187No Hit
CAATGGCTTCGAGCACGTCGATGGGCGTCACCACACCTTGCACCACGTCC11.1627906976744187No Hit
CGCCTTTCCGCCCGAAGCGCAACGGCGCTCGGTGCAGCTTCTCGACGATA11.1627906976744187No Hit
AGCTGCTGCTGTCGCTGAGCCTGCGCTGGGCCCGTGACGCGGGCCTGCCG11.1627906976744187No Hit
GTGCTCACGACCTTCCTGCTGGGGGAAGGCATCACGATCATCACACCCAC11.1627906976744187No Hit
GCTACTTCTTCGCGTCGATGACGCCCTGGACGTAGGCCTGGAAGTCAGTG11.1627906976744187No Hit
GATACGGCGCAAGCCGAACGTGCTCGCTCTGCTGGCGCCGCTGTTGTGTT11.1627906976744187No Hit
TTTCAGGAGTGGGCTCGAAGCTGATGGGTTCCACCTTGCCGAGCAGGAAG11.1627906976744187No Hit
GGCTTCTATGATGCCGCAGTGATGCCCACTGCCCGCGAACGCACCCAACC11.1627906976744187No Hit
CGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGT11.1627906976744187No Hit
CATCGGGATACCCGCCTGTTGCCGCGCCGACGGCGCGTGTGGTCGGCGTG11.1627906976744187No Hit
GCGTCAGCCGCCGAGGCTGGATTGTCGGCGCTGCTGGGCGGTGGCGTATG11.1627906976744187No Hit
GTGGTGGGCTGCAACAGCAACTTCAGTTCCTCGGCCATCACCCGCATCGA11.1627906976744187No Hit
GGCGTCTTGCACTTGCTTGGTCATGATGAAACTCCTGTTTCTCTGTCGTC11.1627906976744187No Hit
GCGCAGATGCAGGCGTACGCCGCGCTGCGGCGGCAGGGCGACAGTCTGGA11.1627906976744187No Hit
CTCTTTTTTTGGGGTGGCGGGAAGGGGACTGAGTTTTGCTCTCGTCGCCC11.1627906976744187No Hit
GTGAGCACTTCAGCCAGTTCATCCAGCGTGCCGAAACCACCGGGCATGAC11.1627906976744187No Hit
ATGTTGGGGGTGCCAACAAATCGCGATTGTTGTTCAGTGCGATACGTCTT11.1627906976744187No Hit
CCCTCAATCTCCTCCGAAAGGATTCCTCGATCACTGGCAATCTCGAACGC11.1627906976744187No Hit
GATAAAAACAGGTAACAATTACGTTAACCAGAATCTTAGTAAATGACACA11.1627906976744187No Hit
TGTCAGACCCGAGCGTTCGGACATCGATTAAGGCCAAGTTGGATCGAGCG11.1627906976744187No Hit
CGTCAAGCACGCCAAGCGGCCGGTGCCGTTTGCCACCTGGCAGTTGCCGT11.1627906976744187No Hit
GCACTCGGGCGTGGTGATCGTCGCCGACGGCACGGACGCTGCGGCCAAGC11.1627906976744187No Hit
ACCAAACCTGAAGCACAACGCCCGCGCGTGGCTTGGCACCAGCCCTGCAC11.1627906976744187No Hit
GTGATGAAGTAACCACCAGTCGGGTGAGTCCAGGATGCGACACCACGTCC11.1627906976744187No Hit
GTATTGGCGGCAGGGGCAATCACGGCGCTGTGGGCCGTCTGCGTGTGGCT11.1627906976744187No Hit
CTACTAAGCCGCGGTATCCGCATCAGCATCGTCTGACATGACAAACGCCC11.1627906976744187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)