FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003620398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003620398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA92.803738317757009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT82.4922118380062304Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG41.2461059190031152Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTTCAATAAGGAACGGGGACACTTACAAATTGCTGCTTGTCCAAATCA41.2461059190031152No Hit
CCTGCAATCCCAGCTACTCGGGAGGCTGAAGTGAAAGAATCACTTGAACC30.9345794392523363No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA30.9345794392523363Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30.9345794392523363Clontech SMART CDS Primer II A (100% over 24bp)
ATCTTGTTCTGTTGCCCAGGCCAAATTGCAGTGATGCAGTCATAGCTCAC30.9345794392523363No Hit
GCAACCTCTACCTCCTGGGCAACAGAGTGAGACTCCATCTCAAAAACAAA30.9345794392523363No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30.9345794392523363Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTCATAGAAAATTTAGACAGAAGCATTCTCAGAACCTTGATTGTGAT30.9345794392523363No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC30.9345794392523363Clontech Universal Primer Mix Long (96% over 26bp)
GATTGAAAGTCTTGGCTTTCATGAATCTGGCAGCTTTCACAATAGTGGAT20.6230529595015576No Hit
ATGTGAGATGGGTTTCCTGAATACAGCACACTGATGGGTCTTGACTCTTT20.6230529595015576No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT20.6230529595015576Clontech Universal Primer Mix Long (96% over 26bp)
GTAAAAATGTTTCAAAAAAATAATAAAATAAATAAATACGAAAAAAAAAA20.6230529595015576No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20.6230529595015576Clontech Universal Primer Mix Long (96% over 26bp)
TCACTGACGGACAGATCTTCTTGGAAACAGAATTGTTCTACAAAGGTATC20.6230529595015576No Hit
GACCTCAAGAGCCCCCAGAAGACAGCTGGGCTCTCCAGGGCCCAGAGATG20.6230529595015576No Hit
CCTATGCGCAGGTGCAGAAGCCACCGAGGCACGCGCCTGGGGCACACGGG20.6230529595015576No Hit
TGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACC20.6230529595015576No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20.6230529595015576Clontech Universal Primer Mix Long (96% over 26bp)
AGCACAGGCTGGTGAAGGTCAAGAAGTAGTAACCTCACTAAATCTTACTT20.6230529595015576No Hit
AGTTCATCATGAACCTGCAGCAACACCCGGCTTGTCAAAGCTGCCTCAGC20.6230529595015576No Hit
CTATGTAGCCATCACCTGAGCTCAGGAGTTGGAGACTAGCTTGGGCAACA20.6230529595015576No Hit
ACTTTAGGTTGGTGGTATCTCCCCTTGTGTAAACAGATCATTAACTGCAA20.6230529595015576No Hit
CCTATTACCATCGCGCCAAGGAACATTGTGATGTCGGCGCCGACGACGAC20.6230529595015576No Hit
ACGCAGAGTACATGGGAATGATACGGCCCACCGAGATACACTCTTCCCTA20.6230529595015576No Hit
GGGTATGGATGAGGAGCTGGAAAGAGATGAGAAGGTATTTCTGCTTGGAG20.6230529595015576No Hit
CTGCATGCCGACGAGACACCTGTTGCCATGTTGGACCCAGGCCGGGGAAA20.6230529595015576No Hit
GCCTAGGTGACAGAGCAACACCCTGTCTCGAGGAGAGAGAGAGAGAGAGA20.6230529595015576No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20.6230529595015576Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC20.6230529595015576No Hit
GAGTTGAGTTCTTGATTTGATTCTCAACTTGGTCACTGTTGGTGTATAGC20.6230529595015576No Hit
CCCTAATTCAAACCCTCCAATTTTTGCTGTACCTTATCATTTCAGGGTAC20.6230529595015576No Hit
GTACATGGGTACGGCGCCCCGAGATCTACACTCTTTCCCTACACGCGGAA20.6230529595015576Illumina Single End PCR Primer 1 (96% over 29bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCC20.6230529595015576Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTA20.6230529595015576No Hit
ACCCAGCCTGTGGCGTTTTACTAAGGCATCCTGAGAAGAGTGATTCACCA20.6230529595015576No Hit
AGACGTATTTGGTATGGAAACATTCTTGCCAGGCTCTCAGATTGACGTGA20.6230529595015576No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG20.6230529595015576Clontech Universal Primer Mix Long (96% over 26bp)
GTGTCTATGTAAGAAGTTGCAGAGCTGGGAGAAGAACTGGGGTCTTCTCA20.6230529595015576No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATG20.6230529595015576No Hit
GGGTCCATCTGCTATAAAGTCTTGGTAAAACAGCATTACTATGAAGAGGA20.6230529595015576No Hit
GCAGAAGCGAAAGCAGGGCAAATTCCTTGGCCGTCAGCTCCAGCCGCTTC20.6230529595015576No Hit
GAATGACTCGAGCTGCTCGTATGCACCTTGAACTCGCTCGAGCAAAGCGT20.6230529595015576No Hit
GCCAGAATCAGCCCAGTACCAATGCTGCTGTTAGTGACTAGGCATGTAAT10.3115264797507788No Hit
TCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGA10.3115264797507788No Hit
CTCTTACACCAGCTTCACTTCACGAAAACCAAGGCTCAGAGAGGTGAAGG10.3115264797507788No Hit
AATAATGGATGCAGGGATATTATTTTATTGCATTGTATTTCATCTTACCC10.3115264797507788No Hit
TCTTGGCTCACTGCCACCTCCGCCTCCTGGGTTCAAGCAATTCTCCTGTC10.3115264797507788No Hit
GAGTACAAGGGAAGCAGTGTTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.3115264797507788No Hit
ATGATAACCACATTGTGAGCAGAAGCCACAACAGTTTTGCCGTCAGCAAA10.3115264797507788No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGCGTTGATACCACT10.3115264797507788No Hit
GCCTTAACCATCTGTCTCAGGCCCGCAATTCAACAGTGAATAGCAGCTAG10.3115264797507788No Hit
GCAGCCCGGTGTGTTCTTCCACCAGTGCCTGGTAATCGCCAATGACTTCG10.3115264797507788No Hit
GGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAA10.3115264797507788No Hit
GTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATACCCAAGCTT10.3115264797507788No Hit
ATCCACGGTGGCGGCCGGGTCCACACGTTCACCGGATTTTCCTCAGATGC10.3115264797507788No Hit
ATCTACACCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGTCCC10.3115264797507788Illumina Single End Sequencing Primer (96% over 29bp)
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA10.3115264797507788Clontech Universal Primer Mix Long (95% over 22bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTC10.3115264797507788Clontech SMART CDS Primer II A (96% over 25bp)
AACTGACTCTGCTCAGGGGCGCTGCCCAGCAAAGTCTCTCCCCGACCACT10.3115264797507788No Hit
CAAGAAACCTGCAGTAACAGGGATGCCTAGAAGGTAGGGGGCTAGAAAAA10.3115264797507788No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAGAAAAAAA10.3115264797507788Clontech Universal Primer Mix Long (95% over 22bp)
TCCGTCGCCTGATGACAGAACAGCCTCAGGGGCTGGGGGCGGCGATCATC10.3115264797507788No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATAACAATGCTT10.3115264797507788No Hit
GCCCAGGGCAGGGGTCAGAGGCCCAGGCCCTGACCCCTGCCCTGGGCCTG10.3115264797507788No Hit
GTCTGTACCTCAGCCATGGCTACACCGCGTTGTTCGATCTGGAGGCGGAC10.3115264797507788No Hit
GATTGGCGTGTGCGTACCGCTGCTGAACGTGGCCGCCGTGTGGCCCATGG10.3115264797507788No Hit
GAGTACATGGGTGATACGGCGACCACCGAGATCTACACTCTTTCCCTACA10.3115264797507788Illumina Single End PCR Primer 1 (100% over 39bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACGCTGCGTTTTTACCAC10.3115264797507788No Hit
GAGCGCGCCAGGCGGACACTCCTCTCGGCTCCTCCCCGGCAGCGGCGGCG10.3115264797507788No Hit
CACCAAGAGATTGGGGGGTGAAGAGCTGGGCACGCATCTGACCTTTCTTC10.3115264797507788No Hit
GAATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA10.3115264797507788No Hit
AGCATTCTCAGAAACTTCTTTGGGATGTTTGCATTCAAGTCACAGAGTAG10.3115264797507788No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG10.3115264797507788Clontech SMART CDS Primer II A (100% over 23bp)
AGACAGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGT10.3115264797507788Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGGTATCAACGCAGAGTACAT10.3115264797507788Clontech Universal Primer Mix Long (95% over 21bp)
GCCTTGGACAGCTTGCGACCGACGCGTTGCGCCACCGCGAGCAGGCGGGG10.3115264797507788No Hit
GTACCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT10.3115264797507788Illumina Single End PCR Primer 1 (100% over 43bp)
CACCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
GCTCTCTACCAGACAATTGAAGAAAATATTAAAATCTTTGAAGAAGAAGA10.3115264797507788No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTGCGTTTTTACCAC10.3115264797507788No Hit
CCAGAGGATGTTGAACTGATGTGGGCCAACCTCCCTGGACACTGTGCTGT10.3115264797507788No Hit
GTGTCGGCCCGTCTACACTCTTTCCCAACACGGACGCACTTCCGATCTCA10.3115264797507788Illumina Single End PCR Primer 1 (95% over 21bp)
AGCATTCTCAGAAACTTCTTTGTGATGTTTGCATTCAAGACACAGAGTAG10.3115264797507788No Hit
ATTAATGACCAACTAGCATCCTCTGCTACATATACAGCCACAGTCACAAG10.3115264797507788No Hit
AGGCTGAAGTGCAATAGTGCGATCTCAGCTCACTGCAACCTCTGCCTCCT10.3115264797507788No Hit
GTATCAACGCAGAGTACATGGGACGCATACTTATAAACTCATAGTACATG10.3115264797507788No Hit
GAAGAGCGTCGTGTAGGGAAAGGTGTAGATCTGGTGGTCCCGTACCATGT10.3115264797507788Illumina Single End PCR Primer 1 (95% over 22bp)
GAGTTTATAGATACCTTCGGAGCTAATCTCTCCGAAGGTATCTATAAACT10.3115264797507788No Hit
CACCAGTCCTTTTAGTAGCTTTTTGATGTGATTTTTAACCAACTTAATGT10.3115264797507788No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.3115264797507788No Hit
GTCCCATGGAGGCTTTAGAAATGGGGACTGATGCTCGAAGAAGGGTCTGG10.3115264797507788No Hit
GTGCAACATTCATGAACATATATGTATGTATGCAAAAATATTTTAATGGG10.3115264797507788No Hit
ACGCAGAGTACATGGGTGATAGCACACCGAGATCTACATTTCCCTGCTGG10.3115264797507788No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
CTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAA10.3115264797507788No Hit
GAACAGGAAACACAAACAATGATTCAGTGCAGCGAAACGAGCTGATCCTC10.3115264797507788No Hit
CTCTTTCAGGAAAAGTGAAATCAGAGAGGCGTACCCTAGCCACAGCTACT10.3115264797507788No Hit
CACAAAAAACCCTTCAAAAAATTAATGAATCCAGGAGCTGGTTTTTTGAA10.3115264797507788No Hit
ACTGAAGGGTTTGGAGAGACAGAATGTGCATGAGGTTTGAGAAGGGGAGC10.3115264797507788No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.3115264797507788Clontech Universal Primer Mix Long (95% over 22bp)
GTCATAGGAAAACAAGAATCACTAAAAGACACTACAGCTGTTCAAGCAGC10.3115264797507788No Hit
GGCCAACCTGGTGAAACCCCGTCTCAACTAAAAATACGAAAATTAGCTGG10.3115264797507788No Hit
TAGCCTAGGTGTGTAATAGGCTATCTCATCTAGGTTTGTGTTAGCACTCT10.3115264797507788No Hit
CTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAG10.3115264797507788No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGTAGCCCACCA10.3115264797507788Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10.3115264797507788Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTGGCAAACCGG10.3115264797507788No Hit
GGGCAAGTGTGTATCCAACAGGTATGCTTTGGTCCTTTGTTCTCCCAAAA10.3115264797507788No Hit
GAAGGAAGAGATTGAGCTACTTCAGGACTTAAAACAAACCTTGTGCTCTT10.3115264797507788No Hit
GTATCAACGCAGAGTACATGGGGCACCGGCTACACTCATCCTACACGACG10.3115264797507788No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAAGGG10.3115264797507788No Hit
GGATTATCTTGTATCTCGTTTCCTTTATATACTAGCTGTTTGGTGAGAAA10.3115264797507788No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
CTGCCACTACTAAAAATACAAAAATTAGCAGGGCATGGTGGTGGGCACCT10.3115264797507788No Hit
AGCTCTGCCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA10.3115264797507788No Hit
CTCTAGTAGTATCATAATCAAATGGAATGAAATGGAATCATCAAATGGAC10.3115264797507788No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAAAGG10.3115264797507788No Hit
GAGTCGGAGAGCGTCGTGAGGGAAAAGTGTAGACTGTGTCGCTCTCCATG10.3115264797507788No Hit
GCCTGCCTACAGCCAGGGGGTCTGAGCTCCGTGGAGTAGGACTGGAGCTG10.3115264797507788No Hit
ATTAAGTAGTATTCAGTGTCCAGTATTAGATAAAGGGAAAAACAAAACTG10.3115264797507788No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAA10.3115264797507788No Hit
AAAACAACCATCTCCAGAGGAAATTAGGGAAAGGGAGCGAGAAAAAGAAC10.3115264797507788No Hit
GTACTGGTATAAAAACAGATACATAGACCAATGGAATAGAATAGACAGCC10.3115264797507788No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTTTACCACTTCT10.3115264797507788No Hit
GTTTCACTCTTAGTCCCATTCTCTTTATATCATTATAAATGGCTCCAATA10.3115264797507788No Hit
CAGTGGTATCAACGCAGAGTACATGAGTGAACGGCGACCACCGAGATCTA10.3115264797507788Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTACATGGGAAGCATTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.3115264797507788No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.3115264797507788Clontech Universal Primer Mix Long (95% over 21bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
GCCAGGAGGAGCAAAAGGGCGAGAGCTCTGCCTCATCCCCTGAGGATCCC10.3115264797507788No Hit
GTACTATGCCATGAGGAAAATAACCCACAGATAAAGCTAAATAGCTTACT10.3115264797507788No Hit
GTCCATGACACCTGAAAACATCATTTGTGGAGTGGCGGGGTGGTGTGTGG10.3115264797507788No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTATTCTGGTCAGTTTCACAGAACATTTCTGGGCTGGCCCTCAGCAT10.3115264797507788No Hit
AGTTAGGAAACGACGCTGAGAGGATGACGACAACGTGCAACAGCAGAAAG10.3115264797507788No Hit
GTGTAGGGAAAGAGTGTAGATCTGGTCGCGACCCCATGTCTGTCTCTTAT10.3115264797507788Illumina Single End PCR Primer 1 (96% over 25bp)
CATCTTTCTCATTCCTCCACCTCATTTGGTGTTTCCCACCCATTATTTTG10.3115264797507788No Hit
TCCGGCGGCTGCACGGGCATCTCGACCGCCGTGAAGTGTCGACGCAACCG10.3115264797507788No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGGTATAAAACGT10.3115264797507788No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCTCTTATACACATC10.3115264797507788Clontech Universal Primer Mix Long (100% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.3115264797507788No Hit
GTCCATGTGTTCAAGGAGTTGACAAGCTAGATGGTAAAGCAAATGTGCCT10.3115264797507788No Hit
CAGTATCTTTTGACTGTATTTCAAATTTCTGTTTTGTTTCCAACAGTTTT10.3115264797507788No Hit
CACGCACTCTGTGTGGTGTTCACATTCCCAACTGCATCTCTTATTAGCAT10.3115264797507788No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATA10.3115264797507788No Hit
TCCTGACTCCATACTCTGATAGGCAATGCACCAAGCTTGTGTCTAGTCTC10.3115264797507788No Hit
GTATCAACGCAGAGTACTGGGAATGATACGGCGACCACCGAGATCTACAC10.3115264797507788Illumina Single End PCR Primer 1 (100% over 29bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGT10.3115264797507788Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGA10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
ACACTTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTT10.3115264797507788Illumina Single End PCR Primer 1 (96% over 28bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
GGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACG10.3115264797507788No Hit
GGGTGAGAGTGGGAGTAAGAGAAGATACTGCAGCTGGTCAAAGCTTCAGT10.3115264797507788No Hit
CTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATT10.3115264797507788No Hit
GTATTTAGATAGTTGGTTACTATTTGGAAATAGAAGGACTCTGAAAATAT10.3115264797507788No Hit
AAAAGAGGCCGGGCACGGTGGCTCACACCTGTAATCCCAGCACTTTGGGA10.3115264797507788No Hit
CTCTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAG10.3115264797507788No Hit
TAAGATACTGGAATTTTTAAGTACATACATTAAATAACTTTGCATGCTAT10.3115264797507788No Hit
CAACCTCCTCTTTTCTACCTAAAAACAGGCTAGAAAATTTCCCATGATAA10.3115264797507788No Hit
GTGTAGTCCCCATGTACTCTGCGTTGATACCACTGCTTCCCCTGTCTCTT10.3115264797507788Clontech Universal Primer Mix Long (96% over 28bp)
GGCCATCTCTCCTGTGCATGCAAAAGAGAACACACATTTGTTCTCTTTAG10.3115264797507788No Hit
GGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTAAAAAAAAAAAAAAA10.3115264797507788Clontech SMART CDS Primer II A (100% over 26bp)
GAGTACTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGTACC10.3115264797507788Clontech Universal Primer Mix Long (95% over 21bp)
GGACATATCAAAGTTGAAATAGGCAAAAGAGAACTATCAGTGTCCCCCTG10.3115264797507788No Hit
GGAAGGGGCCCTCTGATCAAACGCATTGATGCCACAGTGTGCGAGAGCTG10.3115264797507788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTT10.3115264797507788No Hit
GGGCAGATACCACTGTGGGCTACCCTAGCCAGCCTTTTGCAGCCCCATTC10.3115264797507788No Hit
ATGTTTAACATATCTATTAATCAGTGTGTATGAATTAAAAAATCAGAATT10.3115264797507788No Hit
ATATTGGAACAGAGCCAAAGGAACCTTCTTGCACAGCCAAGAGAAGCCAT10.3115264797507788No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAGTACC10.3115264797507788Clontech Universal Primer Mix Long (95% over 21bp)
TCGAAAACCTGCAGCCATCCATGAGTCGCAACTATTACCTGGCGCTGGAA10.3115264797507788No Hit
TCTACACTCTTTCCCTACACGACGCTTTCCGATCTCTGGTACCGACAGAA10.3115264797507788Illumina Single End PCR Primer 1 (96% over 28bp)
ACCGGCTCCCGCCCTGTCGAAGGCCGCGGCCCAGTACCCGAAGCGGTCGC10.3115264797507788No Hit
GTTTTCTCACTGGCCTCTGAAATGTAAATATCTGCCAGTCAGTCATCAAC10.3115264797507788No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
AGTCTACTGTTTCCTCACCTGTTCTCTTTGTCCTTTCTTGTTTCCTTTGT10.3115264797507788No Hit
CAAATGGAATTATCCTCAAATGGAATCGAATGGAATTATCGGATGCAATC10.3115264797507788No Hit
TGGAAGAGGAGGCTAAAGAAGGTTAATTGAAGACAGGACCATGGAAGGTT10.3115264797507788No Hit
ACTTAAGAAACATGCTTATCAGGCTGGGCATGGTGGCTCCCACCTGTAAT10.3115264797507788No Hit
GTGTAGGGAAAGATGTAACTCGGTCTCCCATGTACTCTGCGTTGATACCA10.3115264797507788No Hit
GGATTACAGGCATGAGCCACCGCGCCCGGCCCAGGTGATTTTTTTAAACC10.3115264797507788No Hit
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA10.3115264797507788Clontech Universal Primer Mix Long (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTGGGCATACCGG10.3115264797507788No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTC10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
CTGCAAGAAGCTCAAGGAAGCTCAGGGAAAATGGACGTATTCAGAGAGTG10.3115264797507788No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACC10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.3115264797507788Clontech SMART CDS Primer II A (96% over 26bp)
GGCTAAGATATATGGTTATTTCCATGCTTTCAGGATGTGTTTAACAAATC10.3115264797507788No Hit
CCGTACATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
GTGTCGGCCCGTCTACACACATTACCAACACGGACGCTCATCCGATCTCA10.3115264797507788No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATAACAC10.3115264797507788No Hit
GAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA10.3115264797507788No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATCGGCACC10.3115264797507788Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGTCTTGGCCTGTGTTTCTCTGCAGCTGGGGTCCACCT10.3115264797507788No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTTAAACACAGCGT10.3115264797507788No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAACAAATCATACC10.3115264797507788No Hit
AATGGGAGGTGATTCCTAGGGGGTTGTTTGATCCCGTTTCGTGCAAGAAT10.3115264797507788No Hit
AAGATTCTCTTCGATTCCATTCGATAATTCCGTTTTTTTCCGTTTGGTGT10.3115264797507788No Hit
CCTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAAGAGCGTCGTGTAGG10.3115264797507788Illumina Single End PCR Primer 1 (100% over 23bp)
GATTTCAGTTGTTGCTCCTGGTCTAAATGTCTCTTCTTTGACTTACTATG10.3115264797507788No Hit
GCTGCAGCTCTGGTGGGCATACATTCATTTTAGGAGAGAAACTAAACTCA10.3115264797507788No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
ATTCAGAACTTTTATGCTTCAATGGACACCATCAACAAAGTAAAAAGGTA10.3115264797507788No Hit
GCTAATTTTTTGTGTTTTTAGTAGAGACGAGGTTTCGCCATGTTGGCCAG10.3115264797507788No Hit
GTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCAGAGTA10.3115264797507788Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CTACAAGCACGTGCCACCATGTCTGGCTAAATTTTTGTATTTTTAGTAGA10.3115264797507788No Hit
TTTCAGAGCTGGGGAGAGGAGTTCCTCCCTTCAAAGCCAGCAACTGCCTT10.3115264797507788No Hit
GTACATGGGTGGCACGCAGCTGTAACCCCAGCTACTCGGGAGGCTGAGGC10.3115264797507788No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCT10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
CGCAGAGTACATGGGAAGCAGTGGTATTAACGCAGAGTACATGGGAAGCA10.3115264797507788Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.3115264797507788Clontech Universal Primer Mix Long (95% over 21bp)
ATGGAATACAGACTCAGGGCTTGGTCCATGGGAATTAATATTATGAAAAG10.3115264797507788No Hit
GAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGA10.3115264797507788No Hit
GCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA10.3115264797507788No Hit
GAACAGAGCAGTTGGTACTCAATTCGTCTTTAAAAAAAAAAATCAATGGT10.3115264797507788No Hit
TGGGTGATGCGCCCCGAGATCTACACTCTTTCCCTACACGACGCTCATCC10.3115264797507788Illumina Single End PCR Primer 1 (97% over 37bp)
GGCTGGAGTGCAGTGGTGCGATCTCGACTCACCGCAACCTCCGTCCTAGG10.3115264797507788No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.3115264797507788Clontech Universal Primer Mix Long (96% over 26bp)
ATGTTATTTATTTTGTAAAGTAAATTTCAACTTCCCTGTTCTTTAGTTTA10.3115264797507788No Hit
TTCTAGGGGAGCTGCCTGGGCTGACAGATAGACAGACCTTCTCCCTAACT10.3115264797507788No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCT10.3115264797507788Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGCGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAATT10.3115264797507788No Hit
GAGTACAGTGGCACGATCTCGGCTCACTGCAACCTCCGCCTCCTGGGTTC10.3115264797507788No Hit
GCGTTGATACCACTGCTTCCCATGTACTAAGCGTTGATACCACTGCTTCC10.3115264797507788Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCTAC10.3115264797507788Illumina Single End PCR Primer 1 (100% over 27bp)
CAAAAAAACACAGAAATTTGAGGGACTCATCTTTGCCCAATCTTGTTAAT10.3115264797507788No Hit
GTTCAGTTGTCCGGAAAAACCAGGCAACTAACGAAATAGTCAGTTGTTCG10.3115264797507788No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTGAAAAAA10.3115264797507788No Hit
GTACATGGGTAAGCAGTGGTGTCAACGCAGAGTACATGGGAAGCAGTGGT10.3115264797507788Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGGGGAAGCAGTGGTA10.3115264797507788Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCT10.3115264797507788No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCC10.3115264797507788Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAATTAATTGCGTTGAAACAAAT10.3115264797507788No Hit
AAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10.3115264797507788Clontech Universal Primer Mix Long (95% over 21bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTCTTATA10.3115264797507788Clontech Universal Primer Mix Long (95% over 21bp)
GCTCTATTCACAATAGTAAATATATGGAATCAACCTACTTGCCTATCAAG10.3115264797507788No Hit
GGTAAAACTATGGGACAAGGAGTTGTCTAAGATGTTATTTCCACTGTAAG10.3115264797507788No Hit
ATCGACACCCTAACATCACAATTAGAAGAACTAGAGAAGCAAGAGCAAAC10.3115264797507788No Hit
GAACTAAATAAAGTAAAATAAAATGGTCCTGCAACATTTTAGCCAGAAAG10.3115264797507788No Hit
GCGTCGACCCGCGCGCCTGCCGCACGTTTGCGCGCCGACCTCGAACTGCT10.3115264797507788No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTGAGGG10.3115264797507788No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)