FastQCFastQC Report
Fri 23 Dec 2022
EGAF00003620855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003620855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363870383
Sequences flagged as poor quality0
Sequence length98
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11875840.3263755599476751Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC9528140.2618553321499651Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAA4824730.13259474322206652No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA4595840.1263043164466617No Hit
GCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTG4066120.11174638525059623No Hit
GCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGG4025840.11063939765606041No Hit
CCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAA3990990.1096816390247403No Hit
GGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGAC3676280.10103268009037164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)