FastQCFastQC Report
Tue 28 Jun 2022
EGAF00003620857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003620857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365563808
Sequences flagged as poor quality0
Sequence length98
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG7305450.19984062536081254No Hit
GTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAA7181450.19644860467149966No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC7171110.19616575391401986Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG7129170.19501848498087643Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTG6947160.19003960041908743No Hit
GAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAG5238620.1433024792213566No Hit
GCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGG5206370.14242028029207968No Hit
GTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGA4995490.13665165671980306No Hit
GGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGG4962070.13573745243402213No Hit
GTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGAAAAGA4828710.13208938889267724No Hit
AACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGT4710420.1288535652851061No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA4682960.1281023968324567No Hit
CCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAA4555600.12461846332446563No Hit
GGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGAC4543830.12429649490903652No Hit
GGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAA4518170.12359456546639322No Hit
GAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGA4487040.12274300414334234No Hit
GGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGAAG4462280.12206569420570212No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGA4285680.11723480022398716No Hit
AAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGAA4146330.11342288019934402No Hit
GGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGA4120310.1127111029546995No Hit
GGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGA4056690.11097077750103752No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)