FastQCFastQC Report
Tue 10 May 2022
EGAF00003621219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513062
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT11010.21459394771002333No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG10720.20894160939613537No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC10190.19861147385696076No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC7580.14774042903196885No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC7570.14754552081424857No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA7250.14130845784719975No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC6740.1313681387434657No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG6570.12805469904222103No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC6550.12766488260678047No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT6520.12708015795361963No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA6210.12103800320429109No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA6200.12084309498657081No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA6070.11830928815620724No Hit
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT5870.11441112380180174No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT5810.11324167449548007No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC5750.11207222518915842No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC5740.11187731697143814No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC5700.11109768410055704No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT5500.10719951974615154No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG5400.1052504375689488No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT5400.1052504375689488No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT5330.10388608004490685No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT5290.10310644717402574No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT5230.10193699786770409No Hit
TACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGC5210.10154718143226356No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC5200.10135227321454328No Hit
CCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTC5170.10076754856138245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)