FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003621253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162
Sequences flagged as poor quality0
Sequence length146
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTGTCATGGATCCTCAGATTAGAGTGAATGCGACAGCAGCCCAGTTG42.4691358024691357No Hit
CTCCCGAGGAAATCTCAACTGCACAACCCCTACTATGCCCCAATTCAGCA42.4691358024691357No Hit
GCTCGCAAGCTGGCGCACCTCTCTCAATACGTGCCCCTCGGGTACCGCGA31.8518518518518516No Hit
CCTGAAAAAGCAGTCGGACGCGGCGATTGCGAAACAGCTCGACGACACCA21.2345679012345678No Hit
TCGTAATGCCTGCGTCGTCAGAAAGCTTTCGACTCACGTCAAACAACTCG21.2345679012345678No Hit
GCAGCTTCGACTCCCCCGGTGCGATGGCGACCGTGTCCAGGTATCCCGGC21.2345679012345678No Hit
GCCTCGCGTTCAAGGCCCTCGCCGCGCCCGCCGAAGCCAGCCACGGAGCC21.2345679012345678No Hit
GCACGAGGCTATCCGTGCCAACGCACCGCAGGTCTACGACCAGATCTCCG21.2345679012345678No Hit
GCTATCGAGAGTGGCGCCGACGCCATCCTCATCTCCACGATCATCAGTCA21.2345679012345678No Hit
TTCGAAACGTGGATCGGAGCCTCAGACGAGATGATGCCGCCCTTAGTGGT21.2345679012345678No Hit
AAGTTGACGCAGACTCGATGCGCGCGTTGAGTCGTATTCTTGATTCGGCT21.2345679012345678No Hit
CCCGAGAATCCGCGCGGTGGTCTCAGCACGGCGACGCGCTCGATAATATT21.2345679012345678No Hit
CCTGGAGGGAGTACACCGTCACCCCGGCGCGCTGGGCGGCCTCGCCGACG21.2345679012345678No Hit
GGCAATGATGCACGCCGAGCCTGAGACGTGGCACGCGCTTATGCGGCGCC21.2345679012345678No Hit
GTATTAACCTGAAGTTAAATGTTACTACCAGCATCAAGTTAGTGCTGACT21.2345679012345678No Hit
GACCTTGACTCCACCCTCCGCTCTCCTTCATCTTCCCTCTCGGAGGCCCC21.2345679012345678No Hit
GATAAAAATCGCGCTTGGAAAAGATGAGGCTGAGAATCAAAAGGCTATCG21.2345679012345678No Hit
GAGGCGCGCATCGTGGTCGCGCACGGCCAGATGAGCGAGCAGGTGCTGGA21.2345679012345678No Hit
CAACAAGGACCAGGCAGACCGCGAGCGTTTCGGTTTTCTGGTCAACCCGG21.2345679012345678No Hit
TATCCAGAACATGCTGTCTACACGTGAGGATGCTATTGCAAGGCTGAATG21.2345679012345678No Hit
CGCAGGTGCTGTGCCCGGCGGACTGCGCCCGGATGTCAATGAAGGCTGCT21.2345679012345678No Hit
CTGCTGCCGTGGCTGGACATGGGCGCCACCCCCAACCTGCGGCTAGCGCC21.2345679012345678No Hit
GGACGCCAACGAGGAGCACCTGCAGGTGTTCCTTGAACTCGATGACGGGC21.2345679012345678No Hit
GTTCTATGGCTAAGTGAGAGTGTTCAAGCATTATAGTCTGATTGCCTTGT21.2345679012345678No Hit
CCTCCATAACAATGTCTGAGATACCTTTAATTGATTTAATCTTCTCTGGG21.2345679012345678No Hit
GCCCTCATCTTTAAAACTGGTTTGGAAGTCCGTGTGTGCCCTTTGGGACA21.2345679012345678No Hit
TGCTAGATGAAATGATGATACCATGCCAAAATAAAAGAGAGGCGCAATGC21.2345679012345678No Hit
ACGCTGAAAAGTGGCACCGGCATTCCTTAAGCCGAATGTCATAGTCAGGT21.2345679012345678No Hit
GCCGGCGCGCGGCCGGGCGCTTGGCGCCAGAAGCGAGAGCCCCTCGGGGC21.2345679012345678No Hit
TTTGTAGACTTTTTCGAAAAAAATGTTGCGGCGCAAAAAAAAGAGGAAAA21.2345679012345678No Hit
ATCTGTGACAGTGTCATACAGGTCAGTAGGATTTTGAGGGTTCTTTGAAG21.2345679012345678No Hit
GTGAAAGAGATCGCCCCTCGGTGCGACCTGGTCATTGTTGTTGGCTCTAA10.6172839506172839No Hit
TCCTCGCTGGGGCGCTGGCGGGCGGGACGGCGGCACGGACAGCTGTCAGC10.6172839506172839No Hit
ACTTTTTAGAACGCGCTTGTTTAAAAAGTGCAATCAACGAAATCACAATT10.6172839506172839No Hit
CTATGGAGGCAAAAAACAAGAAAGGAAATATTCTTGTTTAGAAGACCACT10.6172839506172839No Hit
ATACAGCACAAATGAGCTACACGGCATAATTAAGGGTACATATGAACCAC10.6172839506172839No Hit
GGCCTGCACCATGGTGCGCATGATCGAACTCGACCCCGCCGAAACCATCA10.6172839506172839No Hit
ACTGCAGGCCCTCCCTAAACTACATGTCAAGTGTCTCTCTGCTACCATTG10.6172839506172839No Hit
CTGCTAACGAGACCGAGCGTGAGTGTTTTCTGCCCTCGAACCCCCTCTAC10.6172839506172839No Hit
CGCCAGGGCGGTTCCGAGTCCCTGAAGCAGCCGTACCTGTCCACGCCGGT10.6172839506172839No Hit
ACTCCAGGAGCCCGCGAAGGAATGGGTGGATTGGAAACCCGATGCCTCTT10.6172839506172839No Hit
GCATAGATAAATTCGCCGATGAGATCGTCGTCACGCTCGGCCCCGACCGC10.6172839506172839No Hit
ATGAAACGTTCCGTGGACAATGTGGCCACCACAGAAGTGAGCGCGGATTT10.6172839506172839No Hit
TGACTGGCAGATGTCAGCTAGTTTCTCGCCGCGCTCCCACAAAGTCATGG10.6172839506172839No Hit
GGTACCAACAGGTATGTTCTCTTTGGTTCGAATACGACCCATGTTTGAAC10.6172839506172839No Hit
CCTTATAAGTTAATTCGGAGCCATCGGTGTAGTGGGTTTTGTCTACCATC10.6172839506172839No Hit
CTCCGAGACGTATGCGCCGTAGAGCCCGTCACCCCCAAGTTCAACCCCTG10.6172839506172839No Hit
GACTTAAACGCCGCGTCCGCGCTGACGGCCTTGACCACGTCCTGGCAGTC10.6172839506172839No Hit
GCCCCCTCATGCACGTACACGTCCAGCGGCACCTCGCTGGCGATGTCGGC10.6172839506172839No Hit
CTGCAGGGTCTCCAACGTCCACGGCACGTTCGGAATAAACAGGGACGGTA10.6172839506172839No Hit
GAATGTATTTACACGATAAGTCTTCCAAACAACAAGTACAGTTCGCGTTC10.6172839506172839No Hit
GTCCAGCATGGCTTGAGTTGTAATGGTGCTGATTTTCTCGTTGGTGGTTT10.6172839506172839No Hit
GTTAACATGAGTGAAAGCCATTTTAACACCCATGGGTGGCACCCTGTCGT10.6172839506172839No Hit
GTGTCATAATGACATAGTAGGTGTTAGAGCGGGCGCGCGCGAAACATCAC10.6172839506172839No Hit
CATCTCCATCTTCCTGGGTTACCCCTTTGCTTTGTTTTATCGGCATTACC10.6172839506172839No Hit
GTCGTGTCCATCGCGCAGTCCAAGATCGGCTCGCCGTACGTCTACGGCGC10.6172839506172839No Hit
TGTCGAGGATGCGCGCGACCGATTCCGGGGGTGCAACGTCGCAGCCCGTC10.6172839506172839No Hit
CTGGTGTTGTTACACGAGGGCGGGGTGCATGCCGAAGGATCACCCTCGGA10.6172839506172839No Hit
ATCACCTGCTCCACGAATTCACCGCCAGAGGCGGCAAGCTCGAAGTCGCC10.6172839506172839No Hit
GTGCACGACTCCATGTCCACGGCCGCGGCGGTGGAGCCGCGGAGCCACTC10.6172839506172839No Hit
TCCCCGACTGGTTTCAGGCGGGTGAAATCGTCGGACAACATATCGTCGAC10.6172839506172839No Hit
CGCTGGACGTGGGTGATTACTTCACGGTCGCGGGTTTCGCCGTTTTTTCG10.6172839506172839No Hit
CCTGAGGTCTGCACGGCGCAACTTCTTCTTCTTGGGGATGGCCTCCGGAG10.6172839506172839No Hit
CACCTGCTCCACGATGGATAATATCACGCCGCTGGTGCCTGCTACAGGCA10.6172839506172839No Hit
CAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAA10.6172839506172839No Hit
GAGCTTGGCATCCTGAATGGAAAGATTCGTCTCGAAAATCGTCGCGACCA10.6172839506172839No Hit
GTTTCCGTACCTGTTTTTTCAGCGATTGATCATCTTCATGTGCTGTGCAC10.6172839506172839No Hit
GCCTGGTCCTGTCCTCTTGAGCTGCCCTTGCCCAGCACTGCACTCTGGAG10.6172839506172839No Hit
ACAATGTGGCATCTATCAAACATCGATGGGGGTCATTCAAGAGAAAGTGA10.6172839506172839No Hit
GGCTGCTGGTGGTCTCATGAATAATGACCAGCCCTAACCATTGGATTTAG10.6172839506172839No Hit
TACCTACGCAAAACCACAACGGTGATTATCAAGTTTGCTTTTTTAACCTT10.6172839506172839No Hit
CTGCCGCCCCAAGGAGCACGCGACCACCATCCGGGACGTGCATTCGTTCG10.6172839506172839No Hit
CTCATCTGGCGGCCGGACTTCGCGCCGGCGCGCTCGCCTTCGCCGTCGTT10.6172839506172839No Hit
GGGCCAGGAGCCTCTGCAAGGGGCGGCGCTCCATCAGCCGGGTGGCAAGC10.6172839506172839No Hit
TCCTCGCTGGGGCGCTGGCGGGCGGGACGGCGGCACGGACTGCTGTCAGC10.6172839506172839No Hit
ATCGAATCCGTTCCGACTAAAGTAGGAGAGACAGACACCCGCTAGCCCTG10.6172839506172839No Hit
TTGTTCTCCTGGCGCTCCACGTCCGACTTCGCGGCGATGGCGCGCAGGTT10.6172839506172839No Hit
GTCTGGAGTTACGCTGCACCAAAGATCATTTCCAATGTCTTGTTAGCATG10.6172839506172839No Hit
TCTGAGTGACGGTGTACTGGTTGACCCAGCTCTGACGCCACTCGAGGTAG10.6172839506172839No Hit
ATGCAAATGGGAGGTAGAGATGCAAATATGTGTAATAAGAAAATAAGCAT10.6172839506172839No Hit
TCCCTCGAGACCGCCGAGCTCGACCTGCTCGGCCGCGCCCGCAAGGTCGT10.6172839506172839No Hit
AGACGAACAAGATCGGTGCGCTGGCGACACTGAGAAAGAGCCCTTTCGGG10.6172839506172839No Hit
TGCGGGGGCGGCCGCGGTCGTCGCGGCCGGGCTCCTGGCGCAGTCGCAGC10.6172839506172839No Hit
AGGTCGTTGAGAGCCTCGCGTACCAGGACTTCGGCGTCGATCCAGAAAAA10.6172839506172839No Hit
CTTCCTACTTTTCCAACCACTCAAACGGGTTGTCGTTTTCCAGCATGTCT10.6172839506172839No Hit
TGCTGGTAGGGGCCGTCCCAGAAACTGCTCAGGTCATCCGTCACCGCCAG10.6172839506172839No Hit
GCATTGACTGTCGTATCCTCACTATTGTCGCCGTTGATGCGGCGCTTGCT10.6172839506172839No Hit
TTATCATTCACTATGGAGTACACTAGCACCAGCTATTTTCTTGACATCAG10.6172839506172839No Hit
TCCTTCTCCCTCGTTCTGTATTGGGTCCTCTCTTTCCCGGATCCGGAGTC10.6172839506172839No Hit
TCATGGGTCTACTACGGGTGCAAGAAGACCTTGCAGTGCCGCATCTGAGT10.6172839506172839No Hit
ATCCTTCGCCGGAATGAGGTCACGTGCCGGCTCGCAATAGTCAACGCGGA10.6172839506172839No Hit
CACCATGACGGGTAGGTAGGCCGCGACGCCCCGCAGGTCGCCCAGAATCG10.6172839506172839No Hit
ACACTGACCCCCGGCGGTAAGGGAGCTAACCAGGCTCTTGCGGCGGCGCG10.6172839506172839No Hit
AGACGAACAAGATCGGTGCGCTGGCGACACTGAGAAATAGCCCTTTCGGG10.6172839506172839No Hit
CAAGGGCATCGGCTTCACGCCGGCCGGGGATGCGTTGCTGGTGCGCGCGC10.6172839506172839No Hit
AACGTACAACGCCTTGCGCCCGTCCATCGCTGCCCAGCGGGACGCGACCT10.6172839506172839No Hit
CAGTTTCAACCACTTTATAGTGAAAGAGGTTGAAGACTCTGTAGGAGTGT10.6172839506172839No Hit
CGTCCAGCCCGTGCTCCGACCCGACCAGCAGCACATCGCCCACCTGCAGC10.6172839506172839No Hit
AGCCATCTCGCACCGCAACCCCGTGTTGCCTGCCCGCGTGAGCGTGGGCG10.6172839506172839No Hit
CCGACAAGGACCGCCGCGCCCCGGACTTCGCGGCGCAACCGATCACCGCG10.6172839506172839No Hit
CACTAAGGGTGATTCCTCTGGTTTTCCTCCTTGATGTTTTGGACACGAGG10.6172839506172839No Hit
GGGTAGCATGATACCTCCAGCTTTGTTCTTTTTTCTTAGGATTGGCTTGA10.6172839506172839No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGT10.6172839506172839No Hit
CTGGTGGATCTTCGTGGTCAACCCCCCGCGCGAGCGTCCAATGCCGTGAC10.6172839506172839No Hit
GCAGTCGGTTTGGCAACCCAACCCTCCGGCGCGTTGACTACCGTGTAGGT10.6172839506172839No Hit
CTTCATCAGCATCTATACGATGAAAATGCGAGATATCATCTGCTTTTAAT10.6172839506172839No Hit
GTAAAGTTTTGTTTAACTGATTTCTTTGCTCCGCCAACTGACAGGTGGGA10.6172839506172839No Hit
GAATTGTTCTAGTTGCTTGATTGGCAAGAGCACATTGCTATTTAGAGGAA10.6172839506172839No Hit
GTGTTGGGTCATTGGGCAGGATCCACAGACTTTCAACTGAAGCGTAATCA10.6172839506172839No Hit
AATAAGAAACTACTAACAAAGTTCGATGAGATCCATTACACCTCGACAAA10.6172839506172839No Hit
ACCCCCGACTGCCTGGATACCTTCGACACCGATCCGGGTTCAGCTTTGGC10.6172839506172839No Hit
CGACTGAACCCGACTGTCAGCGGGCGTGCGACGCGGGTTGCTCGACGGCA10.6172839506172839No Hit
GTATTGGCTTTCCTATGGACTTAATGTCATCTTGGATCACTAAAGAAATG10.6172839506172839No Hit
CGTTACGTTTGTCTTTCCTGCACAACCGACAACGCCGCGCTGGTTAACCG10.6172839506172839No Hit
CCTAGATGTCAGGAAACCATCAAGAGGAACCCTTTTTTCTTGTAATAAAA10.6172839506172839No Hit
AATCTCATTAGCGTAATCACGCTCATGACCTTCCATTTTATCAATGGTGA10.6172839506172839No Hit
ACGCAGCCTCTGGGTGCATTGAGTGGTCATCTCACCAAGTTACAGCTCCA10.6172839506172839No Hit
CTCCTAATGATGTGATCCCGTTATCAATGACATCTAATGTCCATGGTCAG10.6172839506172839No Hit
GCAATATTGATGCTCTCAATCAGTCGCTCAGAGGACCACTGGTTATTCTC10.6172839506172839No Hit
GGTTAGTATAGTTTGTTAAAATTTTCATTTAGGTGTTAGCATGTTCTTTT10.6172839506172839No Hit
CCCACGGATCAGCGTGGAACAAAAATGTCACGTCGGGGCGGCGGCGGCGC10.6172839506172839No Hit
GCTTATCGACTGTAGACCTTTCCACCTCCTGTGTGCCACTATGGCGACCC10.6172839506172839No Hit
ACTCAAGTACGATCATATTGCCTCCTAGTTGGTGCTTTTATAAGAGAAGA10.6172839506172839No Hit
ATCCAGCACCTCATCGGTGAAGCACAGGGGGTGCGCAGCACCCCTGTATT10.6172839506172839No Hit
GCCTAGGGCAATTGGAGTTATTCCTTTAATAGAAAAGGAATATTCCCAAT10.6172839506172839No Hit
GTACGAGGTGTCAGCGGACAGCTTCTCACCGTAGTACTCGACGCTGTCGT10.6172839506172839No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)