FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003621254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162
Sequences flagged as poor quality0
Sequence length146
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGAGGGGACCCAAAGAGGGTAGCCATTGCCAGCTCGAATGCCTGGGTT42.4691358024691357No Hit
CCCTTAACCGGGAAACGAACAGTGGTGAACTTCGGCATGCTTACCGCCCA31.8518518518518516No Hit
GTTCTTCCTTTTCTTCCTGTATTAAGAGAGAGACAAGTATGGGGCCCCGC31.8518518518518516No Hit
GATGTATGGAGAGTCAACGCACAGTTTCACTGGGATCTTTATCAGAAGAA21.2345679012345678No Hit
CTCGGTGGACCCGGCCCGTGAGCGCGTCGTCGTCGAGGGTGTGAACATTC21.2345679012345678No Hit
GCCGTACATCGCACTGTGCAGCGGAGTGATCATGGGCGGCGGCATGGGCA21.2345679012345678No Hit
GGTGAGAAAGCCCGCCCACGAGTCGAACAGCTGCATCGCGTCGATGCCAG21.2345679012345678No Hit
TCGCTGGCCCCATCGGGTGGATCGTCACCGCGATGATGAGACGGCACCTC21.2345679012345678No Hit
GGATAGACAGGTCACGCGTCAGCATGTTAAACCTCTTCGCGTGCATCAGT21.2345679012345678No Hit
GTCCAAGCCGGTCTCTGCTGCCATCTCCCGGTTCACCTGGGTGCCGCCGG21.2345679012345678No Hit
CTTCGATACCGCCTGACATTGCAATCAGTCTTTCACGCGCCTCAAGGGAT21.2345679012345678No Hit
GTCAAACGCACGACGTAGATGTATGTGGAGCTAGGAAGGCTTATAATATG21.2345679012345678No Hit
GTCGCACTCAGCATCGGGATCAACGACCACTGGCGGCCTATCATCGATGC21.2345679012345678No Hit
ATGTTGTGGTTAAGAAAAGTACAATCGACAATCAAAGATAGTTTGTTTGA21.2345679012345678No Hit
GTCGATGGTGCGCTCGTCCACGCTCGCGCCGTCGCGCGCGGTGAAGATCA21.2345679012345678No Hit
CTGGCTCTACTCTGACAATGAGAAGGCGTCTCGCGTTATAAGTAACGCGG21.2345679012345678No Hit
CTTTCAAAATCATTTTCTTACGATTACAAAGATGACACAAATCGTGTTCT21.2345679012345678No Hit
GGATTGACCAAGTGATAGACTCCACAGCCGGATGCGATCTGTTGAGTTTT21.2345679012345678No Hit
TCCTGGATGAGCCGGTCAACGGCCTCGACCCGCAGGGGGTGGCCTGGATG21.2345679012345678No Hit
GAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCC21.2345679012345678No Hit
GTCTTAGGAGCGGCTGACGTTGAGCTGGTTGCGGCTGACATCGCCGGCAT21.2345679012345678No Hit
GCGCTAAGCAATGCTTGAATTTAAAATATTACTTTCTGTGCGGCCGTTTA21.2345679012345678No Hit
GTTCACTAGCTCCTGGAAGATGCGCTTGTTGCGCACGCGCGGGTTCAACA21.2345679012345678No Hit
CAGCGGTTTATAAGCCCTGAGTGTAGAGACGATGAAGGGGAAGCGCAGTG21.2345679012345678No Hit
GATGAGGACGGCCCCGCCGCACGGTCGGCTTAAAAAAGGACGACCGCCGT21.2345679012345678No Hit
CAGCCCGTCAATGACGCACGGCACCGCGTCGACGGTGGGGGCGTGGATAT21.2345679012345678No Hit
GTCTTAGGATGTTGCTAGCACATCGGGGTGGGTCCCACCCGGTCCACCTG21.2345679012345678No Hit
GAATTCACCCTCCCCGTCCGCAACCTTTGTCGGGTTGCCCACGGGATCCG21.2345679012345678No Hit
ACTGGAATCTATACACATGGAAATCATTCATTAATTGTGCCTCCTTCAAA21.2345679012345678No Hit
AGGTCGGTGAGCTTGGCGAGGAGGTTGTTGACGATTTCTGACATCTTGAT21.2345679012345678No Hit
CTCCTGCGGCTCGAGGCGGATCTCCTGCAGCTCCTTGACCATCTGCTCGT21.2345679012345678No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC10.6172839506172839No Hit
CTATTGATATTGCATGCCATGTCATCAACCGTGTTTATCTTCACAAGCTT10.6172839506172839No Hit
CCTCGACACCGTCGCCGAGGCGTCCCGGGAGCTGTCCGCCCTGGTCGACG10.6172839506172839No Hit
ATCTTGATTTGTATACATGTTAATGCACTGCAATTGTGCTTGGTCAAATT10.6172839506172839No Hit
TCCCAGGGCCTAGCCCTGACACAGGTGGCTTCCCGTATCCCGGTGGGAAA10.6172839506172839No Hit
ATGTAAATAAACGCCCGCTTCGCTCACGCTCATTTCTCACGCCTTACCGC10.6172839506172839No Hit
CTGTGGTGATGTACGAGCAGGCTGTTGAGGCTGGTGACGACAAACTGCAA10.6172839506172839No Hit
GAGACCGCGGACGCCAGGTACGACTCGTACTCCCAGCCGCAGGTTCTGCG10.6172839506172839No Hit
ACCGCGCACATATCATGGACACATCGCACACTAAAACTCTACACATCTTC10.6172839506172839No Hit
GCCACACCCACGTTTCACCACTTATCGCAGAGGGGTCAACGTGGACTACA10.6172839506172839No Hit
GTCCATCTATGGATCTCGGAAGCATGCCCCTACGCAGGATCACGGCCCCC10.6172839506172839No Hit
TGGATGTAGAGCCGGGCGGGCGCGCGGCGGTGTTTGTGAAGTCGCTGCGC10.6172839506172839No Hit
GCACGATGGTGGCGACCGGCGCCAGGGTGCCGGCGGCTTCGAACGCGATC10.6172839506172839No Hit
GCGCGATCATGCGCAACCTCCCCTGGCCCGAGGCCGGTCAGCTGGGCGAG10.6172839506172839No Hit
TCCTGGGACCACGGTGAGTTTAATTTGAATGGACATTACTTGCTAGTCTT10.6172839506172839No Hit
GCCTACTACATGCCCCCAACGGAAACTAGCGCCACCGACCAGCGCGACGC10.6172839506172839No Hit
GTCGCCGCAGCTCCTCCTCTCGCTCCCTTAGCTCTAAGCCAGGTGATTGG10.6172839506172839No Hit
TTCGTGTTGTGTGCGCCACGGACACTTTTAGTGCGATACACGTCACATGT10.6172839506172839No Hit
ATCATGCGAGCGGCGGAAGAGGTCCCCGCAGGCGGTCAGGCTCTCATCGT10.6172839506172839No Hit
ATCATGTGCAGATCACGATAGCTCACCGGGCTACCGGAGTTCAGCGCGTC10.6172839506172839No Hit
CTTCTAGGCTCAGCGCCGCATCCGGCTGGACGCGGCGAAACTCCGTGAGA10.6172839506172839No Hit
CAGATCACCCAGTGGATCGGCCTGCGCACGCCGGCGCTGCAAAACCTCAC10.6172839506172839No Hit
CCCTTAACCGGGAAACGAACAGTGGTGAACTACGGCATGCTTACCGACCA10.6172839506172839No Hit
CGTTTAAAGAAGACCGCGGCGCGCGCTGGCGTGGTTTGTTCGAGCCGGGC10.6172839506172839No Hit
GTATGGTGGAAAAAGGAATAAAATTTATTTTCTGTTTGCGAACCGCCTAT10.6172839506172839No Hit
GCCGTGGATAACCGCCCGCTCACCTTCGACGAGTTCGAGGCCCGCGTGGG10.6172839506172839No Hit
GCTCGTTAGGCCACGATGCTTGACGACGACCCTCCCCCACCGTCAGGCAC10.6172839506172839No Hit
ACTGTGTACACTGCAAAAACAAGCACAAAAAAGAACATGCTAACACCTAA10.6172839506172839No Hit
GTCCGGATGCGGAGGCGCTCGCAGCCTTCAAGCATAACATCCGCGATGAG10.6172839506172839No Hit
GACATGTATATATTATGATAAGCAATAAAGCGGGACCTCTGTCCTTTTTT10.6172839506172839No Hit
GCGCCGCGCATCTCCAAGTCCTTCTGCGCAACGGCCATACCGGCGCCCAA10.6172839506172839No Hit
AACCAACCTTACATGTCCACGTACATGAGACCGGTTCCACCAACTGACAT10.6172839506172839No Hit
CCTGAAATTTTAGATCAGAAGGAGCAAAAAGGAGTTCCCAAAGGATCCCA10.6172839506172839No Hit
AAGATGCACTACGTCAATCAGGTGTAGAAGACTTTAAAATTGCAGAGTTT10.6172839506172839No Hit
GCCGAACATCTCACCCCCCAGCTTCAGCATGACTCGCTTGAAACCTGTGC10.6172839506172839No Hit
CCGTGTAGCCGCGGCGCGCGAGTTCTTCCAACAGCTCTTTCACCAGAATC10.6172839506172839No Hit
GTCGTACTTATCGTTGATACGCCAATTAAGCGCTCTGGTTTGATTCTTAG10.6172839506172839No Hit
CTCCTGGTCGCCTAGGGCCTCCTTTTATAGGCAAAAGGGTCGCCATAGTG10.6172839506172839No Hit
ACCATGACTGATACGATCAATATTGACAACGAGGTTACGCGACTTGCAAA10.6172839506172839No Hit
GTCAATGCACTGGGCATTATGATTTTCACCGCCCAACTCGAACACCTCAT10.6172839506172839No Hit
AAGAGAGGCATTGAAAGCACAAGTGCTCCCTGGGTGATTTTGGTAATTAA10.6172839506172839No Hit
CTCCACTACTCCGCATTGCGGACAATTCCGGCCACACCAATCATTGGTGC10.6172839506172839No Hit
GGTCAACGCGACCGCGACGCACACGATGCCGTCGGGCATCGGCGAGTTGG10.6172839506172839No Hit
GATCAATATCCTGGGCGCCACCTCCGGTGACACGGGATCTTCCGCCGAGT10.6172839506172839No Hit
GGTGCAGTTTCGTCGGATCGTGCTCCCGATGCGCGAGATCGTCTCGACGA10.6172839506172839No Hit
ATCTTATTTTATGATAACAGAAACTTGCTTTCCCTCGTCCCTTTAGACAT10.6172839506172839No Hit
CTGTGCCCTGACCAGACCAGGGCAGGTTGTCCTACGCCAAAGGTTTGAAT10.6172839506172839No Hit
CTCCACTGGAGCTGGATCTTACTTTAACATCTCAACGCATTTTGACACAT10.6172839506172839No Hit
GATTGGCCACAAGTTGTGTCCATTTTGGTTTCCTATTTAATATCACCTAA10.6172839506172839No Hit
ACCTTGGGACGAGACCTGACACGAGCGCGGGTGTACTCGAGGTCGAACCC10.6172839506172839No Hit
GTGGATTGGACGGTCAGCATTGACGCCACGATCAACCGTGCCCACCAACA10.6172839506172839No Hit
GCGCCGCGCATCTCCATGTCCTTCTGCGCTACGGCCATACCGGCGCCCAA10.6172839506172839No Hit
CGCGTCGCTCGGTCTCGTGGACTTATGGCAAGGGTCCAGAATGCCCGGTC10.6172839506172839No Hit
CCGTAGGAATGTTCTGCCATCGGCATGGGCATTCGATGGTTCTAGTGTGG10.6172839506172839No Hit
TCCATTGATAGCTTATTTTGATTCTTCAAACAATGTGCGGAAGATGGTAG10.6172839506172839No Hit
TCCGTCGGCCTCGCGCGTGGCGACGAGAGTTTTTATCGAGTTGGAGCCGA10.6172839506172839No Hit
GCGGTGCTTGCGCTCCTTGAGCTCGACCTCGGTGGCCGCGCCCGCACGGA10.6172839506172839No Hit
CTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAA10.6172839506172839No Hit
CCATATCCATCATGCATCTTGAAATCAATCTAGTTCTAAATAGCGAAGAA10.6172839506172839No Hit
GAACAGTTTACCCGGAAAGTCTTTTCAACAATATTTCCTATTTATGCAAT10.6172839506172839No Hit
CTCGGTGCTCTATTCAGCCTTTCCTCTCACGTAGCTGGCCTACTCAGCCG10.6172839506172839No Hit
GAGCGTGGAGTTCTCGCTGGGTGGCTCCGGTGGTTTGCCTCATTCTGTGT10.6172839506172839No Hit
GGGTGAGGAAGAGGGAGAGGGCGCTGTAGTCAAAACCCTCTCCTCGTCCT10.6172839506172839No Hit
GGGAACCATCATCGACATGTGCCCGGCCTCCTCAGGCCGCGGCGTGCTCA10.6172839506172839No Hit
GGCCTGGACGCACGTATGCGCATCGGCGACTTGATTCTGGGCAACGCGTA10.6172839506172839No Hit
CCCATTAGCGGCCGCGATGACGATAATTGAGTTCTCTCCGGAATCCGCGA10.6172839506172839No Hit
GGCTAGCGGGTGTCTGTCTCTCCTACTTTAGTCGGAACGGATTCGATCTG10.6172839506172839No Hit
AAGCGTGGGTACTCACCTTCACCGAGGGCCTGTCCAACGAGCTGGCCGGC10.6172839506172839No Hit
GATCAGATTTCAAAGGAAGACCGTGCGGATCTTGAGCGCGACGTTCTCGC10.6172839506172839No Hit
ACCAACAACAAGTAGGCAGAGAGCCATCCCATTCACAACTGCTACAAAAT10.6172839506172839No Hit
ACCATAGACCTTTCATTGAGCGCTTTGCCCATGCTTGCTATGACTTTGAG10.6172839506172839No Hit
CTGTACAGCTGATCTTCAACACGTTTGACCAGCTGCGTATGTGGTCTGAT10.6172839506172839No Hit
ATGTCAGGCCGCGACGCCAGCAAGCATGGCCCGGCATCCGCATGTCGATC10.6172839506172839No Hit
GTGCTGGCCAACGAGGAGGTAACCGCGGAGGGCCGCTCCGAGCGCGGCAA10.6172839506172839No Hit
CGGCTACAGGGAAATCATTCCCGAACTGCGCCAGAACTTCTTCTGTTGTC10.6172839506172839No Hit
GGGCCAATACCCATGACGTTGAAGACCCGTTCCTGAACGTCACGGCGATG10.6172839506172839No Hit
GTCAAAACTGAACGCAATGGTAGCAGAGAGACACTTGACATGTAGTTTAG10.6172839506172839No Hit
ACGGTACCCTCAGCGTCACCGTCGATTCCCTCGACCGCCGCACCCCAAAC10.6172839506172839No Hit
GATCATCTCGCAAGCACATACATAACTCAACTCAACATAGTTCATTACAT10.6172839506172839No Hit
CGGTAAAGCCGTCCAAGCTGGAGCTCCCCTCCACGACATCCAGGGCGATG10.6172839506172839No Hit
ATGAAATTGATGCACTATTTCGCGCGCCAGAAACAGTGCGCCTCTTGCTG10.6172839506172839No Hit
CGTGGTCGCAGGGCTTTTTCGCCCGCACCCCGCGCACGTCCGAGGCGATC10.6172839506172839No Hit
ATGTAAATCAACGCCCGCTTCGCTCACGCTCATTTCTCACGCCTTACCGC10.6172839506172839No Hit
GCCCCAGCACGAGTTCGGACTGGACGTCATCGCCCTCATCGGAGCCTTGC10.6172839506172839No Hit
GTGCCAGGCGCTCGTGCGGTGCAGGAACAAGAGGGCGAGGACGAGGAGCA10.6172839506172839No Hit
GCGTTAGCGATAGTTGCAGAAGCAATAGTTGGCGTGACGACCTCGATGCA10.6172839506172839No Hit
GTACTGTAGACAAGAGTGCACTGCATTTTCTTTTGTAGTCTCTTACTACA10.6172839506172839No Hit
GTAGTACGGAAACACAATTACCTATCAGTGAAGAGAGGACAGAGGAGGAA10.6172839506172839No Hit
AACGTTGAGAGTGTTAAACCTGTCCAAGGTTTTGAACATCATTTTTCGAA10.6172839506172839No Hit
TAGTAGAAGTGAGGGCCCTCCGGAGGCCATCCCCAAGAAGAAGAAGTTGC10.6172839506172839No Hit
GTGCCGGATACTTGTCAGGTGATCCCTGCGATCAAAAGTGATCGGGCAGG10.6172839506172839No Hit
GGTTACCGGTGTTGCCGCCGGTGCCTGTGAGCAGCGGGATGAACAGGGAC10.6172839506172839No Hit
CCTTGTAGAAAAGGTAATGCCGATAAAACAAAGCAAAGGGGTAACCCAGG10.6172839506172839No Hit
CGTCAGGGGTAGGTATGATACACTGTACAGGTAGTTGTACCTACCGCAAT10.6172839506172839No Hit
CACGGTGGAACGGGCCGACGAGGAGGTCGGCCCGGTCGACCTCGCTTCCG10.6172839506172839No Hit
TGTTGCCTCCTTAAAAATTAGGGGAAAATGATTTTTAAAGCTCTTCTTAA10.6172839506172839No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)