FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003621398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAGAAGCTGCACCGGTGGTTGCAGCCCTCGGAAATCTTCAGATACGC33.8461538461538463No Hit
GAAGTGACCACTCTGCGCCTATAAGAGCGTAGGCGGGTGAGCGTAGTCGC33.8461538461538463No Hit
GCATAGACCAGCGAGCTGGCGAAGGGCCGGCTGCTGTTCTGCCGGCTGCG33.8461538461538463No Hit
TTTGTTGGCTGCATAGATGTCTTCTTCTAAGAAGTGTCTGTTCATATTCT33.8461538461538463No Hit
GGTGCTGGTTGGCGACAACCCCGCCAGCCAGGTCTATGTGCGCAACAAGG33.8461538461538463No Hit
CCCTCACCAATGCGGACCAGCGTATTGGCAGATCCAATGCGCTCAGCACG22.564102564102564No Hit
GTCGAACGTCCAGCGGGCGGCCTTCACGGCAACGGTATCGCCGCTGATGT22.564102564102564No Hit
GAAACATTCACTAAGACAAACTAATGTTTAAAATTATCCTGTGCCCCGGT22.564102564102564No Hit
TCGAAGCCCATCGACGAGAAGATAGCCTCGATGCGCTCCATCGCGCGGGT22.564102564102564No Hit
GGACTCTGGATCATTGGAAGTAAGCTGACATCTGCTTGAAGGGAAACTGA22.564102564102564No Hit
GGCGAAAAGATACTTTGAAATGTAACATCGTTTTTCACTTCAAAATCAAG22.564102564102564No Hit
GTATACGGGCTACTCGACCCATTTCTGATAGGCTTGAGGTTATTTCCTTT22.564102564102564No Hit
TCTTACTGTGCGTGAAAATGCGGCCTACGGGCTGAAGTTGCAAGGCAAGC22.564102564102564No Hit
CAGAGGCTACCAGGCAGGTAGATGGCAGGGAGGGGTATATCCAGAACCAA22.564102564102564No Hit
GGCCAACTCCCACATCGTTGCCGGCGACGACCTCTACGGTGGCAGCTACC11.282051282051282No Hit
ATCGATACGACGCGCGTGTCGGAGCCCAATGTGCGCAACGCACTGCAGTA11.282051282051282No Hit
ACGCGGGCCGCCGAGGGCAGGCTGGAGCAGCTGGCCAGCAGGTACGGGTG11.282051282051282No Hit
GTTAAAATATCTTGGCCTAATAAGGGGACTGGGCAGGTGGGGATAACCAG11.282051282051282No Hit
GGTCTCCGCCGGGGGAGCGGTGCGTGTCTCCGCCCGCACGCCGGCACAGC11.282051282051282No Hit
ACGTTTGATGCTGCACTTCAAGCTGCGAACGAAAAAGCTGCGATCGCCGC11.282051282051282No Hit
AAACAGTGTTGTTCGATTGAGAAGTTTCAGCTTCGCCGTGAGCGTAAAAT11.282051282051282No Hit
TAGAGACACAGTGAGGAGAGAAAGATTCAGGCCAGACACCTATCCTGTGA11.282051282051282No Hit
GCCCACCTGGGCGAGTTTGGTTCGCGAGAGGTCATTGCCCAGGCTAAGGG11.282051282051282No Hit
GTTACAAGTCCTTATTCCACCAGACGCATGATTAGGTAATTTATGAAATA11.282051282051282No Hit
ATTTATTCTAGCGTATGGTCATTCTCCTGATAAAATTGTTCATTTTGCAA11.282051282051282No Hit
GTTTCCTTTACAGCCTGCAGTCATTAGGGGACACAGTCAAAAAGGGAAGC11.282051282051282No Hit
CCTTGAGGAGCAGGGGCCGTACCACCTCGCAGGCCTTAATCAGTCCGGCC11.282051282051282No Hit
CATCTGTGGAACGCCCCGCGAGCCGAGCGCCTTCTCGCGGGGCGTGTCAC11.282051282051282No Hit
TTTCAGCTCTATGGTGAGAAAGGAAATATCTTCAAATAAAAACTAGACAG11.282051282051282No Hit
GCCACCACCGTTCAGAAGAGCACAGCACTCGTCACCCACCTGCCACTGTG11.282051282051282No Hit
GGCGAAAAGATACTTTGAAATGTAACATCGTGATTCACTTCAAAATCAAG11.282051282051282No Hit
TCGCGTGAGTGGTCGTCATGTTGCGCTGCGCCATGGCATATCGGCTAGGT11.282051282051282No Hit
CATTATTTGCTGTCCCACCAATTAATGGCAGATGTCTTAGTCTGTGGCAC11.282051282051282No Hit
ACCTTGGGCAGCTCTGGCCCTGTGGCTTTGCAGGGTATAGTCCCCCTCCT11.282051282051282No Hit
GAGTCGAAGCGCCTGGGCGTGAAGGTGTACGGCGGTCAAGCCATCAACGC11.282051282051282No Hit
GTGTGATGGCTGCATTCCACACACACGGTGGAACATTTCTCTTGATAGAG11.282051282051282No Hit
AGAGGAGCCTGTCCGCCCCCTCACCCACGACCTGCTTGCGCGTGTCATCG11.282051282051282No Hit
GCCCGCATCGATGCCGAATCCGCCATCGACCAGGCACGCGAGCGGGATGT11.282051282051282No Hit
GACAGAGTCTTGCTCGGAGTGCAGTGGTGCCGTCTCAGCTCACTGCAACC11.282051282051282No Hit
ACCTTCATCTACAACACGAAGGCGGATAATGGTCACGACGTCATTAAAGA11.282051282051282No Hit
CCCCGCAATTGCAGCGCGCCGCCATGTTGCTTGAGACGCACCAGCGCGAC11.282051282051282No Hit
GATCCCCGATAAAATCCCTGGACACCAAGGCTTGGGTGAGCCTCCCTCAT11.282051282051282No Hit
GAGTGAGACCTTGTCTTACAAAAAAAAAAAAAAAAAAAAAGAAAGAAAGA11.282051282051282No Hit
GGGCCAGTACCCCCGCTGGACCATGTACGTCCAGACCATGACCGTTGAGC11.282051282051282No Hit
GCTCTGTCACCCAGGCTGGAGTGCAGTGGCACGATCTCGGCTCACTGCAA11.282051282051282No Hit
GAGTGAGACCTTGTCTTACAAAAAAAAAAAAAAAAAAAAAAAAAGAAAGA11.282051282051282No Hit
CCCAGAGACACCCCGGCTCTAAGCTGTCGTGATTTCCGGGCAGCAGGTAC11.282051282051282No Hit
GTTCTGTGGCCTTGTTCATTTTTCACTTACGTCCTGCCTCTGCCACAGGG11.282051282051282No Hit
GAGGAAAAGCGCGCAATCTCGGCCGCCGAGGACTGCACGCACACGCTGCA11.282051282051282No Hit
ATTCCATTCCATTCCATTCCATTCCATTCCGTTCCATTCCATTCCATTAC11.282051282051282No Hit
GCCGAACAGGACGCCGAGGTTCACGGTCGGCACGAGCACCTGCCCAGTCA11.282051282051282No Hit
TTCGTCCGGAATGCGCCGAAGAATTTGCCATGCTCGAACAACACGTGACG11.282051282051282No Hit
ATCTCCACCGGCGGCGGTGCGTTCCTCGAATTCCTGGAAGGCAAGACGCT11.282051282051282No Hit
GTAAAGAGGAGCCAAGTGCTAATATCCAAGATAATGGGGAAAAGGCCTCC11.282051282051282No Hit
ACCTGGGTATCCACCTCGGCGCGGATGCGGCGCAGCGCATCCTCCCGCTC11.282051282051282No Hit
GCCTTGGCCTGGCCGATCGATTTGGAGCGGGCGTTACGCTCGGCCTGCAG11.282051282051282No Hit
GTCCGTCAGACAAGAGTGAGGCAGAACTCCAGTGGCTATTCCATCCCAGA11.282051282051282No Hit
GTCAAGCGGTGGAACAGCTTGGGGTCAGCATTCCCTCTCCGCCAACACGG11.282051282051282No Hit
CACTATATGCATACACCAGACGTGCACACAAACACACCCACATACAACAC11.282051282051282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)