FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003621439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA60.25619128949615716No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG60.25619128949615716No Hit
TGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA50.2134927412467976No Hit
GTATCAACGCAGAGTACATGGGACTCTGGGCAGGATCCAACGTCGCTCCA40.1707941929974381No Hit
ACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCC40.1707941929974381No Hit
CCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGA40.1707941929974381No Hit
GTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT40.1707941929974381No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG40.1707941929974381No Hit
GGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGC40.1707941929974381No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1707941929974381No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT40.1707941929974381No Hit
GCTACAGTTGTGATTGGAGGAGTTGTGGCTGTGCCCATGGTGCTCAGTGC30.12809564474807858No Hit
TATCAACGCAGAGTACATGGGAACTCGGTGGTGGCCACTGCGCAGACCAG30.12809564474807858No Hit
CATTTATTGTTGCACAAGCCTGTTGCAGTCCTGAGGGGATCTTCTGGCAG30.12809564474807858No Hit
GCGTAATGTGCGTGTGAAGAGACTGGGGGAGCTGGCCGGGGCTCACGGTG30.12809564474807858No Hit
GGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGC30.12809564474807858No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC30.12809564474807858No Hit
GGCATAGGTAAGCGGAAGGGTACAGCCAATGCCCGAATGCCAGAGAAGGT30.12809564474807858No Hit
CTCCACAGATACCCCGAAGCCATGGCAAGCAAGGGCTTGCAGGACCTGAA30.12809564474807858No Hit
GCCTTGTGGCTACTCTGCAGTCACTGGGAGCAACTGGACTCTCCGGATTG30.12809564474807858No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG30.12809564474807858No Hit
CTTTTACTCATCTGCCTCATTTTGGGCCTGGTTGGCCCTGGGCTTTAGTC30.12809564474807858No Hit
GACTTACACACAGGTCAATCCTGGGCGGCGACAAGACAGCTCTAGAGATC30.12809564474807858No Hit
CGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGAC30.12809564474807858No Hit
ATCACGAGGTCAGGAGATCGAGACCATCCTGCCCAACATGGGGAAACCCT30.12809564474807858No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCT30.12809564474807858No Hit
GCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTC30.12809564474807858No Hit
GTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCA30.12809564474807858No Hit
CTGGATGCGCTGGTGGCGCGCGAAAAGGGTGCCCGGCGTGAGACCAAGGT30.12809564474807858No Hit
ACTTGATCTTCCGGGGCAATGCCCTCCAGTGAGGCTACATGAGCCTTGAT30.12809564474807858No Hit
TCCTTCACCAACATGCAAGTTCTTTCCTTCCCTGCCAGCCAGATAGATAG30.12809564474807858No Hit
ATGAGGTTCTGCCCGTTTGCTGAGAGGACGCGTCTAGTCCTGAAGGCCAA30.12809564474807858No Hit
TTGAAATCCTGCGCGATCTCCCTCACCAACCTCTGGAAGGGCAGCTTCCG30.12809564474807858No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC30.12809564474807858No Hit
GCTGGTGTGAGTCAATTAACCAAGGCAGGGAGGGGACTCAATGTTTTACA30.12809564474807858No Hit
TCTGAGATGTGGGCTTGCACACGCTGTTGCTATAGTACGTGTTGATCCTG30.12809564474807858No Hit
GTGTGGAAAAATTCATTTTATCTTGGTTGAGCTGGAAATTTTTTTCTGAT30.12809564474807858No Hit
CTTTATTGATAAGCATTAGTGAACCCCTTGCCCTGGGAGCTCAGCCAGTC30.12809564474807858No Hit
TCTTGCAGGAGGTGCATTTGCACTCTTTGCACTTGCAGGAGCCGGTGCAG30.12809564474807858No Hit
CCCAGAGGGTGTCTCCATCTTCCCTATTTATTTTCAGCCCTTGAGGGCTT30.12809564474807858No Hit
GTCCTCCTCCCAGCGACCCCACGCTGGACCCCCTGCCGGACCCTCCACTG30.12809564474807858No Hit
GAAGAAATCCTGACACCCAGTGGATCACCAAACCAGTCCACAAGCACAGG30.12809564474807858No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA30.12809564474807858No Hit
GGCCTGGAAGATCAGCACTGGGATGACGATGAGCAGAATGGTCATGAGGA30.12809564474807858No Hit
CTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCTGCAAATGCA30.12809564474807858No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG30.12809564474807858No Hit
ATCGTAGTTTTCTGGGGTAGAAAATGTAGCCCATTTCTTGCCACCTCATG30.12809564474807858No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA30.12809564474807858No Hit
CCTCCGCGGTCGCCAAAAAGAAAGATAAGGTGAAGAAGGGCGGCCCGGGG30.12809564474807858No Hit
CCCTGGGAGACAGTGATAATGAAAGCTAAGCCTCGGGCTAATTTCCCCAT30.12809564474807858No Hit
GAGCGCAGCCAGCCATGGTCGGCATAACCGCGTTCCTGTGATTTACGGAC30.12809564474807858No Hit
CTGGGGAGAAGCCCATCCAGCTGGGGTCTGACGGGTAAGAGAAATTCATT30.12809564474807858No Hit
GTATCAACGCAGAGTACATGGGAAGCAGCCTGAGGTGATCTGTGAAAATG30.12809564474807858No Hit
TAGATAGACAGATGGGAAAGGCAGGCGCGGCCTTCGTTGTCAGTAGTTCT30.12809564474807858No Hit
GGCAGGGCGAGCGCGGCTGGGGGCGTAGCGCGCTGAGGGGGTCCGGCCGT30.12809564474807858No Hit
AGTCATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCA30.12809564474807858No Hit
TCTTTGCATTGTTCCTCATCCGCCTCCTTGCTCGCCGCAGTCGCCTCCGC30.12809564474807858No Hit
AAGGAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGG30.12809564474807858No Hit
GCATAATTGTTACACGTTTGGCATGGATAGCACACAGGTTGGTGTCTTCA30.12809564474807858No Hit
GTCCAACTGGGCTGCCTTACTCTCCAGTGCCCGCTCATTTTCCTCATAGG30.12809564474807858No Hit
CCTGTAGGCTGGCAGAGGACAGTGGAGCAGCCAACACACAAAACTACCGT30.12809564474807858No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)