FastQCFastQC Report
Tue 10 May 2022
EGAF00003621458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365361
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG33330.9122484337408754No Hit
AGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCT30730.841085939659679No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG26350.7212045073228943No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC26310.7201096997216452No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG24090.6593478778523159No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT23960.6557897531482562No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT22290.610081535796103No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA20610.5640996165436377No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC16710.4573558754218431No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC15320.4193113112784342No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA14760.40398400486094577No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA14660.4012469858578228No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC13920.38099304523471306No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT12710.3478751152969255No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG11720.3207786271660084No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT11400.312020166356015No Hit
ACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC11390.3117464644557027No Hit
CCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCC11290.3090094454525798No Hit
CTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGA11000.3010720903435233No Hit
CTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTC10890.298061369440088No Hit
GTATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGA10780.2950506485366528No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC10600.29012401433103147No Hit
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT10570.2893029086300946No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG10560.28902920672978233No Hit
GCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATG10490.2871132934275963No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACAGGC10280.2813655535210381No Hit
CTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCA10200.27917593831853976No Hit
GCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCA10070.2756178136144799No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT10020.2742493041129185No Hit
ATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTT9550.2613853147982406No Hit
ATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGT9230.25262685398824725No Hit
AATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTT9080.24852132548356282No Hit
TCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACT8730.23894175897263256No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG8690.2378469513713834No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG8600.23538363426857273No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA8330.22799368296014078No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT8300.2271725772592039No Hit
CCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACAT8000.2189615202498351No Hit
TTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGC7830.2143085879445261No Hit
CAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGG7700.2107504632404663No Hit
GGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGC7570.20719233853640648No Hit
CTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCT7560.20691863663609417No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC7490.2050027233339081No Hit
CACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCT7310.2000760891282868No Hit
TTTGTGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC6990.1913176283182934No Hit
GACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGC6960.19049652261735653No Hit
GGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTGTGTCCATGAG6930.18967541691641965No Hit
AGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCT6920.18940171501610734No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG6900.18885431121548277No Hit
GTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATC6880.18830690741485817No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT6860.1877595036142336No Hit
GTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGC6850.18748580171392132No Hit
GGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATC6760.18502248461111065No Hit
GCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACAC6750.18474878271079836No Hit
AGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACACCCTCCAGTTA6720.18392767700986148No Hit
TGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGC6620.18119065800673856No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT6570.17982214850517708No Hit
TAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTC6460.17681142760174184No Hit
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC6320.17297960099736973No Hit
GGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAG6150.16832666869206073No Hit
GAACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG6140.16805296679174844No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC6050.1655896496889378No Hit
GATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACAC6040.16531594778862552No Hit
ACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGA6000.16422114018737632No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACAC5900.1614841211842534No Hit
GCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTT5880.1609367173836288No Hit
CTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGT5870.1606630154833165No Hit
TGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGAT5780.15819969838050585No Hit
GGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATG5770.15792599648019356No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG5760.15765229457988125No Hit
TATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAA5700.1560100831780075No Hit
CTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTT5520.1510834489723862No Hit
CTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGA5510.15080974707207392No Hit
CCTCATAACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGA5370.1469779204677018No Hit
TGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGA5320.14560941096614036No Hit
GCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGA5310.14533570906582804No Hit
GCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAG5290.14478830526520345No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT5260.14396719956426657No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA5170.14150388246145593No Hit
CTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCG5160.14123018056114364No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG5000.13685095015614693No Hit
GAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTT4950.13548244065458545No Hit
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG4870.13329282545208712No Hit
TCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCC4870.13329282545208712No Hit
CTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCT4860.1330191235517748No Hit
CCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTC4860.1330191235517748No Hit
TTTGCGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC4850.13274542165146253No Hit
ACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC4840.13247171975115024No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG4720.1291872969474027No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA4660.12754508554552896No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG4640.12699768174490436No Hit
TAACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGAAAGCT4630.12672397984459205No Hit
GTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGC4600.1259028741436552No Hit
GCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAAT4590.12562917224334288No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT4530.12398696084146912No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT4480.12261845133990765No Hit
ATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC4470.12234474943959535No Hit
CTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATG4460.12207104753928306No Hit
CCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGG4350.11906032663584784No Hit
GACTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCA4350.11906032663584784No Hit
CTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGC4280.11714441333366178No Hit
GCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCAC4260.1165970095330372No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA4240.11604960573241259No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG4240.11604960573241259No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA4220.115502201931788No Hit
GGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC4190.11468109623085114No Hit
CCCTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTG4160.11385999052991425No Hit
GGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGG4140.11331258672928966No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA4040.11057556772616672No Hit
TTTGAGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC4010.10975446202522986No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG4000.10948076012491754No Hit
CTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATG3990.10920705822460525No Hit
TTTTCGAGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGG3980.10893335632429296No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG3960.10838595252366838No Hit
CTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCC3930.1075648468227315No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG3910.10701744302210689No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG3890.10647003922148232No Hit
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA3890.10647003922148232No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT3760.10291191451742249No Hit
CTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATCAGGGCTGAAAAGTT3750.10263821261711019No Hit
CCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGC3740.1023645107167979No Hit
GTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATCAG3710.10154340501586102No Hit
CCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGC3700.10126970311554874No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT3680.10072229931492413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)