FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003621538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA115.188679245283019Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG73.30188679245283Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG52.358490566037736Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG52.358490566037736Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC41.8867924528301887Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41.8867924528301887Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT31.4150943396226416Clontech SMART CDS Primer II A (100% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31.4150943396226416Clontech Universal Primer Mix Long (96% over 26bp)
AGTAAAGGCTGGCGCCTTCGCTTTGGCCGGGCGCGTCGAACACCACCGAA20.9433962264150944No Hit
GTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAGAGTAC20.9433962264150944Clontech Universal Primer Mix Long (96% over 28bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTT20.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
CAGCAACAGGCGCGGCTTCAGCAGATCGAACAGCGTCTGCAGGAGCTCAA20.9433962264150944No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCGATCTATCCCTTTTTTGGTACCAACAGGCCCATGTACTCTGCGTT20.9433962264150944No Hit
TATCAACGCAGAGTACATGGGTAGGGCACCGGACTACACTCTTTCCCTAC20.9433962264150944No Hit
CTTTCCCTCACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGGGAC20.9433962264150944No Hit
TCATGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTTCTTGGTA20.9433962264150944No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG20.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC20.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA20.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTACGCAGAGTACATGGGAAGCA20.9433962264150944Clontech SMART CDS Primer II A (96% over 25bp)
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT20.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
CCATGAACGTGGACATGGGGGCGGCCACCATGGACATCGAGGTCCTGCGC20.9433962264150944No Hit
GGCGGCGCACAACCAGCACATCGCCGGAAGGCCCGTCGAACTGCTGCGGC20.9433962264150944No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTAGGGAAAGTGGTCGCCGTTCATTCCCATGTACTCTGCGTTGATACC20.9433962264150944No Hit
AAATGTACCTAATAACATCTTAACCATAGCAAAGAAAACTTGTATTGCGT20.9433962264150944No Hit
TCTTTGCCCTAATCGGTTTCTGGATTCAGACGAAAGCCACCCGGATTTCA20.9433962264150944No Hit
ACACGACGCTCTTCCGATCTCACTGTCTACTGGTACCAGCAGGGAGATCG10.4716981132075472No Hit
GGGCTTTGCCGCGTTGACCGGTGGCGCGGTGCGCCTGCGCGGCTACCGCA10.4716981132075472No Hit
CATCCCGCTGTGGGCCCAGCGCCTGCGCGACGACGACGAGCGCTGGCAGA10.4716981132075472No Hit
ACACCGAGTCTACACTCTTCCCTACACGACGCTGATCGGAAGAGCGTCGT10.4716981132075472No Hit
CTTCGGCAGCCACCAGTTGGCCTTGCCGAGCAGCTCCATCGTCGCGGGGA10.4716981132075472No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAGTACTCTGCGTTGAT10.4716981132075472No Hit
GTGCTCATTGGTTAACTCTAGTTAACCCGAAGATCGGAAGCTGTCTCTTA10.4716981132075472No Hit
GCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA10.4716981132075472No Hit
GATGCTCACGCAGCGCAAGGCGCGCGTGCACGTGACCGGCCAGCGGCATG10.4716981132075472No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.4716981132075472Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4716981132075472No Hit
ATCTGAGGCAAGAGCGTCGTGTAGGGAAAGAGTGTAATCTCGTGTCGCCT10.4716981132075472Illumina Single End PCR Primer 1 (96% over 27bp)
GTATCAACGCAGAGTACATGGGAATGTACGGCGACCACCGAGATCTACAC10.4716981132075472Illumina Single End PCR Primer 1 (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10.4716981132075472Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCTTCCCATGTACCCTGCGTTGATA10.4716981132075472No Hit
CCACAGATCTCTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAG10.4716981132075472Illumina Single End PCR Primer 1 (96% over 32bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
CAGGTAGATGTTCTAAGATACAGCTGCTAGCTGAAGTCCCAGGCAGCAAC10.4716981132075472No Hit
GGCCACAGCCTCATCTCCTTTCTGCCTGGCCCCTTTCAAGCTTTTAGGGT10.4716981132075472No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATA10.4716981132075472Clontech Universal Primer Mix Long (96% over 25bp)
TCCTTGGTGAAAGGCTATTTTCCCTACATCATCCAGGGAAGTTATGTTAC10.4716981132075472No Hit
GAGTACATGGGTGATACGGCGCACCGAGATCTACATCTTTTACAGCAACT10.4716981132075472No Hit
ATATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGAATC10.4716981132075472Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
GATCTCGTGGTGCGACCCATGTACTCTGCGTTGATACCACTGCTTCCCAT10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
CATGTCGACGGGACACCGAGATTACACTTCCCTACACGACGGTATGCCCG10.4716981132075472No Hit
GCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCGTCGGCTGCTTGAGG10.4716981132075472No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.4716981132075472Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGCACATGGGAAGCAGTGGTA10.4716981132075472Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGTGACCTACACTCTTTCCCTACACGACGCTCCTCCCGGGTCT10.4716981132075472Illumina Single End PCR Primer 1 (96% over 30bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTGCGTTTTTACCCCTGC10.4716981132075472No Hit
GTATCAACGCAGAGTACATGGGTCGCACCCGGGTTTAACTATTCCCGACA10.4716981132075472No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATCT10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAGTACTCT10.4716981132075472Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACATGGGGAAGCGCAGTTATACAAATTTCCCTAAACGACGC10.4716981132075472No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTGCGTTTTTAACACGTGGTACCA10.4716981132075472No Hit
ATGGGAAGCAGTGGTATCAACGCAAAGTACTTTTTTTTTTTTTTTTTTTT10.4716981132075472Clontech SMART CDS Primer II A (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.4716981132075472Clontech SMART CDS Primer II A (100% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC10.4716981132075472Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCAC10.4716981132075472No Hit
TATCGACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAA10.4716981132075472No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTAT10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTGACGGATCGACAGTGAAAGTGACCATTCCAGGAGTAGATCAACC10.4716981132075472No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAACAAAGA10.4716981132075472No Hit
CGTTGAGGATGTGCAGGGGTGGTCAACAGCCGAGGTCGCGGTTCGCCACG10.4716981132075472No Hit
ACCTCTTTCCTACACGACGCTCTTCCGATCTCATGATGCAGCAACTCCAG10.4716981132075472Illumina Single End PCR Primer 1 (100% over 23bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGGACCCGACGCTCTTCCGATCTCCCTGCTGGTACCAGTAG10.4716981132075472No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
GGCCACGAGATACACTCTTTCCTCACGACGCTCTTCCGAGGAAAGAGTGT10.4716981132075472No Hit
GAAACATTCTCATAAACTTCTTTGTGATGAGTGCATTCAAATCAAGGAGT10.4716981132075472No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
GGCTTGAACTGGGACAAACAGGTTCAATTTTTTCATGCACAGCTCAAATT10.4716981132075472No Hit
GTACATGGGAAGCAGTGGTATCAACGCCGAGTACATGGGAAGCAGTGGTA10.4716981132075472Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGATCGACCCCGAGACTACACTCTTTCCCTACACGACGATCGGAAGAGCG10.4716981132075472Illumina Single End PCR Primer 1 (95% over 24bp)
AAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA10.4716981132075472Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.4716981132075472Clontech SMART CDS Primer II A (100% over 21bp)
CATCATTACCGAAACATTTGATTCCGAAACGCTTGCCTTTTTATTTTAAG10.4716981132075472No Hit
GGTTTGGGAAATAACCTTTTGGCAGACGTAATGATCAATGGTGCCAAAGT10.4716981132075472No Hit
CCCGGATCTACCTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCG10.4716981132075472Illumina Single End PCR Primer 1 (95% over 24bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGTGTAGACCGCTGT10.4716981132075472Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTA10.4716981132075472No Hit
GTCTATATTAGTTCCTCCTCCCTATCTGAACATCTGAAAATTGTAATGCC10.4716981132075472No Hit
CATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTCCCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCG10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAATGATCTACACTCTTTCCCTACACGACGCTCTT10.4716981132075472Illumina Single End PCR Primer 1 (96% over 32bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTCGTTGATACCACTTC10.4716981132075472No Hit
GTACATGGGAAGCAGTGGTATCAACGCTTCCCATGTACTCTGCGTTGATA10.4716981132075472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTT10.4716981132075472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4716981132075472No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGT10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACAGGGGAAGCAGTGGTATCAACGCAAAA10.4716981132075472Clontech Universal Primer Mix Long (95% over 24bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTGGCGTTTATACCACTGCTTCCC10.4716981132075472No Hit
GTCCTTCTGCGATCGGCTGTTGTCGAACATCAGCGTCATGTTGGCGATCT10.4716981132075472No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
CCGTACATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTGATGGCGACACCGAGATCTACACTCTTTCCCTACACGACG10.4716981132075472Illumina Single End PCR Primer 1 (100% over 32bp)
GTGCTGTTGGCCAGCAGCCGCGAGGGCGAGGAGGCCATGCTGCTGTCCGC10.4716981132075472No Hit
GTGGTATCAACGCAGAGTACATGGGAATGATACGCCACCACCCAGATATA10.4716981132075472No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAATTA10.4716981132075472Clontech Universal Primer Mix Long (95% over 21bp)
AGTTACCACCGGCTTTTGCCGCGGTTGGGATGGTGTTTGGTGCTGCGCGG10.4716981132075472No Hit
GTGTTACGGTAATGGTACCATCGTTATCCCCAAGACATATATCGTCAGTA10.4716981132075472No Hit
GTACATGGGAAGCAGTGGTATCAACACAGAGTACATGGAAGCAGTGGTAT10.4716981132075472Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCTATTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATTAGGCAGGA10.4716981132075472No Hit
CGCAGAGTACATGGGAAGACGCGACCACGAGTCTAACTCTTTCCCTACAC10.4716981132075472No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.4716981132075472Clontech Universal Primer Mix Long (95% over 22bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGGGAAGGTGTGATCGGGTCCTACACCCATGTACTCTGCGTTGATA10.4716981132075472No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.4716981132075472Clontech Universal Primer Mix Long (95% over 22bp)
ACGCAGAGTACATGGGAAGCCGAGATCTACATCTTCCCTACACGACGCTC10.4716981132075472No Hit
GAAGAGCGTCGTGTAGGGAAAGGTGAGACTCGGTGCCTACCCATGTACTC10.4716981132075472Illumina Single End PCR Primer 1 (95% over 22bp)
CGCAAAAAAAAAAAATAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT10.4716981132075472Clontech SMART CDS Primer II A (100% over 22bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGAACTCTGCGTTGAT10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAA10.4716981132075472No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTGGCCGCTGCCGAGTCCTTGGCCGCCGTCATCTCCGCGGACGGAGC10.4716981132075472No Hit
CGTCCCGCCACGGGAACTTGCGTGTCGCGGCAAGCGCGATCGTCACGGCC10.4716981132075472No Hit
CCCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCAGTCCC10.4716981132075472Illumina Single End PCR Primer 1 (97% over 43bp)
GTATAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
GAGCTGCTGGACTCGATGGCGTACATGATGGGTGGTCGTGCGGCCGAGGA10.4716981132075472No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAGCAGTGGTATC10.4716981132075472Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTAACCACAGG10.4716981132075472No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGAGTACATGGGAAGCAGTGG10.4716981132075472Clontech Universal Primer Mix Long (95% over 22bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.4716981132075472Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGGTACATG10.4716981132075472Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTATTCTGCGTTGAT10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTAGGTTAATTCCCCTCGCTCCTTTGTGCTGCTCCAAGTTCGTGGTT10.4716981132075472No Hit
ACGCTCTTCCGATCTATCCCTTTTTTGGTACCAACAGGCCCAAAAAAGGG10.4716981132075472No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTGAGTACATGG10.4716981132075472Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGCGGATACCCATGTACTCTGCGTTGATACCACTGCTACCCCTGTCTCTT10.4716981132075472Clontech SMART CDS Primer II A (96% over 25bp)
CCGTATCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGTACACTGCGTTGATACC10.4716981132075472No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGGCCT10.4716981132075472Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTGGACCATGTCTACGACGTGAACTTCAAAGGCGTGTGGCTGGCGTT10.4716981132075472No Hit
CACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTGGGGAAG10.4716981132075472No Hit
GCAGCTCGTGTAGGGAAAGAGTGTAATCTCGGGATCACGCATGCTGTCTC10.4716981132075472No Hit
TCTCGTGGTCCCGTACCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.4716981132075472Clontech Universal Primer Mix Long (95% over 21bp)
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG10.4716981132075472No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTGCGTT10.4716981132075472No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTA10.4716981132075472Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
CCTCACCAGTATTTGTCGGGTGTTCAGGCCTAGGGACGAACACACATCTT10.4716981132075472No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACGAAGCAGTGGTATCAA10.4716981132075472No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTTTGCGTTGAT10.4716981132075472Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGATAACGGCGACACCGAGATACACTCTTTCCCTAC10.4716981132075472No Hit
GAGTACATGGATGATACGCGACCCCGAGATCTAACACGCTCTTCCGATCC10.4716981132075472No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)