FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003621737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG211.76470588235294Clontech Universal Primer Mix Long (96% over 26bp)
GTAAAGCGCTGGGCGGGGCTTCTGGTGGTTATACCGCGGCGCGCAAAGAA211.76470588235294No Hit
GGTCGTTGCTCCTTGAAATGGGTCATGCAAGGCGCGCCACTCAGATGCGC15.88235294117647No Hit
CCTCAGGTGATCCACCTGCCTCTGCCTCCCACAGTGCTGGGATTACAGAC15.88235294117647No Hit
TTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATT15.88235294117647No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCCATTAAAAATCCTT15.88235294117647No Hit
TTATTATTGTTTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTA15.88235294117647No Hit
TCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGG15.88235294117647No Hit
CGGTTGCAGTCCATTGGCACCGAGAACACCGAGGAGAACCGGCGCTTCTA15.88235294117647No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT15.88235294117647Clontech Universal Primer Mix Long (96% over 26bp)
AGTAAGGCTTGTGTGTCTGCACCTTGGTGGCATGAGAAGCGGTGAGTTCT15.88235294117647No Hit
GCACAAATATGGGTACTAGTATGCCGTCTGAGACAGAACCATGACATTGT15.88235294117647No Hit
TTACTGATCAGAGAAAAATAAATCTACAGTTTGGAATTTTTCTTAAATTA15.88235294117647No Hit
GTCCAGGATTGTCAGACAGGTCTAGGCTGGTAAGGGCCGGGAAGGCGCGA15.88235294117647No Hit
CACCTAGAACAGTGCCGTGCTGCTGCTGGGAAGTTGCTTTACACAACACA15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)