FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003621738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT211.76470588235294Clontech Universal Primer Mix Long (96% over 26bp)
GGTATGCGCCGCCGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAA211.76470588235294No Hit
CACATTGCCAGGGCATGAGTGTCTTGACCAGGACTGCCCCGCACTTCCCA15.88235294117647No Hit
TCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT15.88235294117647No Hit
GGTTCAGCCTATGACCCAGGTCCAAAAGTTCCAGCCTTCTCTGCCACCTC15.88235294117647No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC15.88235294117647No Hit
CGCCCGTTCGTGTGGCGTGCGATATCGGACCTGCACGCGCCACCGCGGCG15.88235294117647No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG15.88235294117647Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGACGTCACTTCCGAGGCGGGAGGATGAAGTTGATTGACTATG15.88235294117647No Hit
CAGGCACACGCTGCCACGCCCAGCTAATTTTTGTATTTTTTGTAGAGACA15.88235294117647No Hit
ATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTC15.88235294117647No Hit
CTTTTAAGGGTTAGAGTAATCTTTTTCATTAGTCTTGGCTATTTCCTCTA15.88235294117647No Hit
GTGTGAAGCCTGGAAGCCGGCGGGTGCCGCTGTGTAGGAAAGAAGCTAAA15.88235294117647No Hit
GTGAAGTGGTGACTCACACAGGTAATGTAATTGTTAGATTTTTTAGCAAA15.88235294117647No Hit
CTCCTCATGAGAAAACTTCTGAGTGCAAGCATGGCCTGGGGTGACCATGT15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)