FastQCFastQC Report
Tue 10 May 2022
EGAF00003621767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64329
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCACAAGCGTAGATCTCGTATG13942.1669853409815167No Hit
CTTATACACATCTCCGAGCCCACGAGACTCACAAGCGTAGATCGCGTATG9561.4861104633990891No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8221.277806277106748Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACTCACAAGCGTAGATCTCGTATGCCG7841.2187349406954873No Hit
ATACACATCTCCGAGCCCACGAGACTCACAAGCGTAGATCGCGTATGCCG5440.8456528159927871No Hit
ACACATCTCCGAGCCCACGAGACTCACAAGCGTAGATCTCGTATGCCGTC4840.752382284817112No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4750.7383917051407608Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACTCACAAGCGTAGATCTCGTATGCCGTCTTC4530.7041925103763466RNA PCR Primer, Index 47 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTCACAAGCGTAGATCGCGTATGCCGTC3580.5565141693481944No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2790.43370796996688893Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2790.43370796996688893Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2770.4305989522610331Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2600.40417230176125857Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACTCACAAGCGTAGATCGCGTATGCCGTCTTC2170.3373284210853581No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2170.3373284210853581Clontech Universal Primer Mix Long (96% over 26bp)
TCTCCGAGCCCACGAGACTCACAAGCGTAGATCTCGTATGCCGTCTTCTG2060.320228823703151Illumina PCR Primer Index 9 (95% over 23bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC2020.3140107882914393Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1740.27048454040945763Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1620.2518304341743226Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1560.24250338105675512Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC1520.23628534564504344Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1350.20985869514526886Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1350.20985869514526886Clontech SMART CDS Primer II A (100% over 26bp)
CACATCTCCGAGCCCACGAGACTCACAAGCGTAGATCTCGTATGCCGTCT1320.20519516858648507RNA PCR Primer, Index 47 (95% over 21bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA1290.20053164202770132Clontech SMART CDS Primer II A (96% over 26bp)
TCTCCGAGCCCACGAGACTCACAAGCGTAGATCGCGTATGCCGTCTTCTG1180.18343204464549426RNA PCR Primer, Index 28 (95% over 22bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1070.16633244726328716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA1070.16633244726328716Clontech Universal Primer Mix Long (95% over 21bp)
CACATCTCCGAGCCCACGAGACTCACAAGCGTAGATCGCGTATGCCGTCT1030.1601144118515755No Hit
TTATACACATCTCCGAGCCCACGAGACTCACAAGCGTAGATCTCGTATGC1010.15700539414571965No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG980.1523418675869359Clontech Universal Primer Mix Long (96% over 26bp)
ACATCTCCGAGCCCACGAGACTCACAAGCGTAGATCTCGTATGCCGTCTT920.14301481446936842No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC820.12746972594008923Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA800.1243607082342334Clontech Universal Primer Mix Long (95% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT800.1243607082342334Clontech Universal Primer Mix Long (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG770.11969718167544965Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG760.11814267282252172Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC760.11814267282252172Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA740.11503365511666588Clontech Universal Primer Mix Long (96% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA730.11347914626373798Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA730.11347914626373798Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT710.11037012855788214Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA680.10570660199909838Clontech Universal Primer Mix Long (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACTCACAAGCGTAGATCTCGTATGCC670.10415209314617047No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC670.10415209314617047Clontech Universal Primer Mix Long (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTCACAAGCGTAGATCGCGTATGC660.10259758429324253No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA650.10104307544031464Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)