FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003621779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTAACTCGTTTAATATGACGATTGACCGGATGCCATGCCGTCAGATG33.5294117647058822No Hit
GATCGGAAGAGCGTCGTGTAGGGAAGATTAACTCGTGGTCGCCCCATGTA33.5294117647058822Illumina Single End PCR Primer 1 (96% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA22.3529411764705883Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG22.3529411764705883Clontech Universal Primer Mix Long (96% over 26bp)
CTCCCAAGTAGCTGAGATTACAGGTGCAAGCCACCACGCCCAGCTAATTT22.3529411764705883No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22.3529411764705883Clontech Universal Primer Mix Long (96% over 26bp)
CAATAAAGGCTTTGCTAGTTGTGTTTCAATCACCTTTGACTGGGAAAGAT22.3529411764705883No Hit
CCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCT11.1764705882352942No Hit
TGTATATAGAATCACAGAAGAATTTCAGAAAGAGCAGTGCCACATAGGAA11.1764705882352942No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT11.1764705882352942Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT11.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACTAGCACCCAGGCCCTTCCCTGGGTGCTAGTCAGTTAACGTGCGTTAA11.1764705882352942No Hit
ATTACAGTCAAATTGTCCTAAGCAAATCTCTCATTGTCTCCATGTAAAAG11.1764705882352942No Hit
GACTCGTCGTATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACCCCCCATGTACTCTGCGTTGATA11.1764705882352942No Hit
GTGTACGGCGCACCGAGATCTACACTCATCCAAAAACAAGCAATTGCCAC11.1764705882352942No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11.1764705882352942Clontech SMART CDS Primer II A (96% over 26bp)
TAAAAGAACAATGTGAACAATTATTGAGCTTCATCTTCTGGACAAGAATG11.1764705882352942No Hit
ATATTATTGAAATAAATTAAAGACAACCTAAATAAATAGGGGGATATAAC11.1764705882352942No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
CGTTCGTGCTCGTGCTCGTGGCCGTGCGACTCCGCCAGGTGCAGCGTCAC11.1764705882352942No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAAACTACATAACAAGCAAAAGAATAAACTAATACACAACTCCTAGC11.1764705882352942No Hit
AAGCAAACTACATAACAAGCAAAAGAATAAACTAATACACAAATCCTAGC11.1764705882352942No Hit
CAAAATCTAAAGCAAACATAAAAACTCATATAAAAATCTAAGAACACACA11.1764705882352942No Hit
GTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTA11.1764705882352942No Hit
GTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTACATCCCATGTACT11.1764705882352942Illumina Single End PCR Primer 1 (97% over 37bp)
AGCCCAAGAGCGGCGCGCCGGTGCTGCGCGTGCATGGCACGACCGGCCTC11.1764705882352942No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
CTGTTGCTGTGCCGCCAGCGCCGCCAGTGCGGCCGCCGTCTGTTCGTCCG11.1764705882352942No Hit
GAATTTGAATTTTGCTCAAATTCTTTTTTTGCTTATTTAGATTTGTGAAG11.1764705882352942No Hit
CCCACAGGAAGTGCACCTTCACGCGGCCGTGGTCGTCCACGTGCAGCTCG11.1764705882352942No Hit
GTGTAGATCTCGGTGGTCGCCGTATCTTACCCATGTACTCTGCGTCTGTC11.1764705882352942Illumina Single End PCR Primer 1 (96% over 28bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1764705882352942Clontech Universal Primer Mix Long (95% over 22bp)
ACACAGTACTGACCTACCTCCAGACCATGGTATTTTATAACCTCTTTATT11.1764705882352942No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGATCGACACGAGATCTAAT11.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTCTTATTTGAGAAATGCATTTTGTAGGCTATAGCTGCCACGGATAGTG11.1764705882352942No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAACAAAGCCC11.1764705882352942No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGTATCAACGCAGAGTACATGG11.1764705882352942No Hit
GATAATGGCCCCTAGATGGTGGGTTTTATCTCCTAAGCTCCATTTTTAAG11.1764705882352942No Hit
CTTAAAATCTTAAGCTATTAAAATTACGTTAAAAACTTAACGCTAAGCAA11.1764705882352942No Hit
GACAGGTGTAGGTCTCGGTGGTCGCCGTATCATTTCCATCTGTCTCTTAT11.1764705882352942Illumina Single End PCR Primer 1 (96% over 29bp)
TCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCACTGC11.1764705882352942Clontech SMART CDS Primer II A (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAAT11.1764705882352942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTG11.1764705882352942No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAGTACTC11.1764705882352942Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACAGTGTACA11.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATCGCGCA11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
CCCTCGGACTGCCCCAAGGGTGCGGTGAAGAGGTGACGGAGGGCTGGCTA11.1764705882352942No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGAGGGACC11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATG11.1764705882352942No Hit
GTACTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGTACTC11.1764705882352942Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACATGGGCGGGCACGAGTCTACCTGTCTCTTATA11.1764705882352942No Hit
GGGCAGACGTGTGCCTTCCGATCTCTTCGTTAACCGCTCTTAACAAAGCA11.1764705882352942No Hit
ATCATGGCCACTGCACTCCAGCCTAGGTGACAGAGTGAGACTTCATTTCA11.1764705882352942No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGTCGCCCACCGAGATCTAC11.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTCAGAGGATACTGTGGCTTGTTTTGTTTATGTTTTTTTTTCTTATTC11.1764705882352942No Hit
GTGTAGATCCGGTGTGGCATTCCCATGTACTCTGCGTTGATACCACTGCC11.1764705882352942Clontech SMART CDS Primer II A (95% over 24bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA11.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCAGTACTCTGCGTTGATACTGTCTCTTATA11.1764705882352942Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC11.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGGTGTACGCGACCCGAGATCTACACTCTTTCCCAACACGCGCTCTACC11.1764705882352942Illumina Single End PCR Primer 1 (96% over 27bp)
AACGCAGAGTACATGGGAAGCAGTCGTATCAACGCAGAGTACATGGGAAG11.1764705882352942Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACACAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG11.1764705882352942Illumina Single End PCR Primer 1 (100% over 22bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACG11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
TTTGTGGGTCTGACCTCAGGACGGATTTTCAATAGTCAATACTACCTCTG11.1764705882352942No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAG11.1764705882352942No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
GGTTGGGCAAGTATCCTACAATTTGTTTCTTGAGTTTTATCTTTTTCTTA11.1764705882352942No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGTGTACTTTTTT11.1764705882352942Clontech SMART CDS Primer II A (96% over 26bp)
GTAGGGAAAAGTGTAGATCTCGGTGCGCCGTCACCCATGTCTGTCTCTTA11.1764705882352942No Hit
GGTAGTAGTTTGCCTCGGCCTCAGCCGGTGGGATGTAGCCCAGTGGCCCC11.1764705882352942No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)