FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003621787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86
Sequences flagged as poor quality0
Sequence length140
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAATTTCTGTGAGATGGATGGCCAGTGCAAGCGTGACTTGAAGTGTTG33.488372093023256No Hit
GCCGCGCAGCCGGTCGAGCAGGTCGACGGTCACGCGGCGCCGCCGCCGGG33.488372093023256No Hit
GGCATGGGGGGTGGTGTGAATGCCCACCGAGGTCTGAAGTGGGGTGCAGA33.488372093023256No Hit
GTGGACGTCACCGGCGACGACAGCGTGGCCGCCGGCTTCGAACACGCCCG22.3255813953488373No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG22.3255813953488373No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22.3255813953488373Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCCACAGGCTCCAGGTCGCCAGCGCGGTGAGGGCGAGCCACCAGAGGAT22.3255813953488373No Hit
GTCTAGACAGGTCTGTGATACTGGCGTGATGCTGCCGTGACTCCGGTGTG22.3255813953488373No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
TAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTC22.3255813953488373No Hit
CACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTT22.3255813953488373No Hit
GGGCTTCGCCGGCGCCATGAAGCGCTGGAACTTCGGCGGTCTGCGCGCGA22.3255813953488373No Hit
GGCGGTTACGCGCCGGCTCAGGATGGGACTAGCGCCCGCAGCGTCTCCGC22.3255813953488373No Hit
GGCCTGGGTCGCCGACTGGTGGCCTGGTTCGACGACCTGGCGCGCGGCAC11.1627906976744187No Hit
GTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGG11.1627906976744187No Hit
GGTCGAGGATGCTCTGGCCGGCGCGCGAGTCCAGGGCGGCGTCGGGGTCG11.1627906976744187No Hit
CAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTG11.1627906976744187No Hit
GCTCAATTTGGCCATCTTGCTTTTTAATACTATTTCTTAACACTAGAATT11.1627906976744187No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11.1627906976744187Clontech SMART CDS Primer II A (96% over 26bp)
ACCTGGCACTGTCCCCGGAGCGGGTCGCGGCCGGCCGGCGCCCCGCCGGG11.1627906976744187No Hit
TCACACATATTGTCCAATCCGTGTGGAACAGCTAGGGGCGGGGTGCCGGC11.1627906976744187No Hit
CCCGGTGCGAGCGCGTCGGCGATGGCGCGCCTCCGCGCGTTCGGTTCGGG11.1627906976744187No Hit
CTCATCTCCAGAACCATCTCCGTGTGTTTCTGGCTCATGTGGAGACCCAT11.1627906976744187No Hit
GCTGAGCTGCGTCCGCGGGCGGGCGGGACCCGGTCGCCCGCGTGAAGCGC11.1627906976744187No Hit
GAGAAAGATCCGAGGCGGTGTTTAGAGGAAGGCAAACTGGTCAACAAGTG11.1627906976744187No Hit
GCTCCAGCATCTTCACCTTCTTCTCGGCTGCGCCCAAGGCCACCACCTCT11.1627906976744187No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG11.1627906976744187No Hit
AGGAAGAAGTCGCCGGCGAGGCGGAAACCGGCGATACGGCCGCCCTGCAC11.1627906976744187No Hit
TTTTTGTAGCTGCCTTTTAAACATTTGATTTATTCCATTTTATATATGTT11.1627906976744187No Hit
GCTCATTCTTCATTGGTCAAAGCAAGTTATCTGGCCATGACTAACTTTAA11.1627906976744187No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG11.1627906976744187Clontech Universal Primer Mix Long (96% over 26bp)
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT11.1627906976744187No Hit
CTTCTGCGGCGTCGTCTAGGTCGAGGCCCAGGAGGCGGCCGCGGGCTCGA11.1627906976744187No Hit
GCGGAATCCGCAACATGTCCAAGACGCCCTCGGCGATCTGGGTCGTGGAC11.1627906976744187No Hit
GCATAGCAGGGACAAAGTTTAACTAATTACATCATGATGGAAAGAGGTAA11.1627906976744187No Hit
CCTCTACGTCGAGCACGGACTGGCGGAGATGCCCGGACGCGCGCGCGCCG11.1627906976744187No Hit
GAGCAGGCTGCGCAGCACCACCAGGGCATTGCCCCTCTCCATCTGGATGC11.1627906976744187No Hit
CCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTA11.1627906976744187No Hit
GTAGAGGAGCGTCTACTGGTCTTTCAACTCCTTCAGTCTTCTGATGGCGG11.1627906976744187No Hit
GGCCCAACGCTGGGGAGCAGCCAGGCCAGGTGGCGGGCGCAGACTTCGAC11.1627906976744187No Hit
GTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTC11.1627906976744187No Hit
CTCTACGGTTGCACGCTCCACGCGGGTCCGACGAGCGGAGCCGGGTTCCG11.1627906976744187No Hit
CACCGGCCATGCCGTCGGCGCGGTCGAGGACGTCGATCAGCCCGGCGAGG11.1627906976744187No Hit
TCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCG11.1627906976744187No Hit
CGGGGGGAGCAGGCTCAACGGCGACCACCCCCGGCAGCCGAGCAATGGCT11.1627906976744187No Hit
TCTAGTCTCTTTGCAACAGTGTGGGGACCTGCTGACGCGGCTAAAGCAGA11.1627906976744187No Hit
GCATTAAGGGTAGTCTTACGACTGTCTTTAACCCAGGCACCCTAATTACA11.1627906976744187No Hit
GGAAGAGGATCAGCGAACCGCCGCCGGCGATGGAGTTGATGACGCCGGCC11.1627906976744187No Hit
GTGCAGATCGCGCCGAACAAAGAAGGCGGGCGCACGACGCCTTCTGTCGT11.1627906976744187No Hit
ATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCA11.1627906976744187No Hit
CCCATAGCCAGCCCTCCGTCACCTCTTCACCGCACCCTCGGACTGCCCCA11.1627906976744187No Hit
GAAGACGGCCGATGCGACGAGACCGAGGAGCGCGGCGAGCCACCACTCCA11.1627906976744187No Hit
CCATAGGGATTGGAACCCTGATCTACGGGCACTGGAGCATAATGAAGTGG11.1627906976744187No Hit
GACCGCCCGTTGACGGTGACGGCTGGCGACTACATCTGGTGCTCCTCTGT11.1627906976744187No Hit
CTCCTGCAGTCCGGTGTAGCGCAGGAAGAGGGCGTACTCCTCGTCGGCGG11.1627906976744187No Hit
GGCTTCTTGGGCTTGGAGGCCTTGACCGCGGCCGGCTCCGTCGCGGACCG11.1627906976744187No Hit
AGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAAC11.1627906976744187No Hit
CTTCCAGCTCATCCGCAGGACTCGGATTAGCTGGAAGTGACCGGTGGCAA11.1627906976744187No Hit
GGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAG11.1627906976744187No Hit
CCACCAGGAGTGGAGCCTGCGGCTTAATTTGACGCAACACGGGAAACCTC11.1627906976744187No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGAATAACAAAAAAA11.1627906976744187No Hit
GAACACTGAAACCTATGAGTTGCGCTCCGCCGCCCTCGCGGAATGCCTTC11.1627906976744187No Hit
CCACGGCCTGACCCGCACGCTCATCAACAACAACATCATCGGCGTCACCC11.1627906976744187No Hit
GAATAGGACTTTATTAGCATTGGTGCAGACATTGTATTACACAGGAATGG11.1627906976744187No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1627906976744187No Hit
CAACGGGTTTGGGCGCATCGGACGTAACTTTGCGCGATGCTGGCTGACGA11.1627906976744187No Hit
GACCACCGCCGTGCAGTCGAACTGGTCGATCACGGCCGGGCCGCTCAGGG11.1627906976744187No Hit
CTCCAGCGCCATCTCCGACGCCTTTCGCGTGGCGACGGTGGGCGGACTCT11.1627906976744187No Hit
GGCTCGCCCGGGTGGTCGGGCTCGCCGCGATCACGCTGCTCTGCGGGGCG11.1627906976744187No Hit
GAACTGCTTCTCCAGCACGCCGTAGATGCGGCGGGCCTTCTGCTTCTCGC11.1627906976744187No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)