FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003621789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length140
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCCACCTTCACGACAGGCGCGTCCTTCGGCATGACGATGACGGCGCG35.660377358490567No Hit
GTGCGACACGCCGATCTCGTCCGACACCGCAATCCGTTGCGACCGCCGCC35.660377358490567No Hit
GTAATGGCATTGCTGGATCAAATGTTATTTCTAGTTCTAGATCCTTGAGG35.660377358490567No Hit
GCCACCTTGGCCGGGTCGTCCCAGCAGGCCGCGCCCAGCTCCTTGCGGTG23.7735849056603774No Hit
CCGCACGCGACGCGGGCGCCAGCGTGTTCGCCTATCACGTGACCGACCCC23.7735849056603774No Hit
CATAGAAGGCGAGCTTGGCGGGCGGCGCGTCAGCCGCAAACGCCTTGATC23.7735849056603774No Hit
GCGCTGGAGCGCAGCCAGCTTGCCAACGATGTGGTCGTCACCCGCGACGG23.7735849056603774No Hit
CAGCAATGTGTCCGACAGCGACGGCGGGGCGATCATCGCTGCGCTGCAGC23.7735849056603774No Hit
GGGCCGGGTGCCAACTGTGCAAGCGCGAGGCCTTCCTTGTAGTCAGCCTC23.7735849056603774No Hit
ATAGCGAGCTGCCGATGATGCTGTACGTGTACGCGGCCATCGTGATCCTG23.7735849056603774No Hit
GGGGGAACCAGCTCCAAAACGTCTGCCAGCCAGTACGCGCTGTTTGTGGA11.8867924528301887No Hit
TGTTTGTCTTCGCGGCGGCCGGAGAGGATCTCGTCGCCGATGATGATCAT11.8867924528301887No Hit
AATATCCACTTGCATATTCCACAAAAAGAGTGTTGCAAAACTGCTCTCTC11.8867924528301887No Hit
GGTCTGGGGTCGGACGCGCATGCGTGTTTTCATCTTCAATCAGGCGCCAT11.8867924528301887No Hit
ACTTCAAGGGCGTGCCTTCGCCGGTCAAAGAGCCGGAGAAGACCGACATG11.8867924528301887No Hit
TCCAGGCGCCACTCGTCCGGAGATTGCCATTCGACCCGCTCGGCGCCGTA11.8867924528301887No Hit
CGCCCCACTGCCCGACTGCCCAGCCGTGCCTTTCATCGGCAAAGGAGACA11.8867924528301887No Hit
GCTTCCCCGTGCAGCAGTCACACATCAGCCGCATGGCCGATGCGGTGCGC11.8867924528301887No Hit
TCATCTTCGTAGTGCTGTTCGTGCTGTTTCTGGTGTTGGGCGTCACGGTC11.8867924528301887No Hit
ATTCCGTACCACACCACAAAAGAGCCCGAGGGAGGGAACCATGAAGCTCA11.8867924528301887No Hit
ACGTGGACAAGGTCCGCAAGGCGATGTACGGCCAGCAGTTCAAGGCACCG11.8867924528301887No Hit
CGCCAACACAGCCCATCACCAGTGCCGCTTTGCCGGTTTCGCGCTCTTCT11.8867924528301887No Hit
ATCTCAATACACACCTCGATGCCGTGGGCCAGATAGGGGCGGCAGGTGCA11.8867924528301887No Hit
GTAGGACCACAGATTCGACACCGACGCCTTGTATCCGCTGGCTGAGGTAA11.8867924528301887No Hit
ATCCGCGCTCGTTGAGCACGGGCACTACGTCAGCCAGCACGGGCGTGACG11.8867924528301887No Hit
GTGGAAGGTAAGCAAATGACCGATGTTGTGATCGTAACGGCGGAACGCAC11.8867924528301887No Hit
TGCCGGGACGGCGTCCCGGTAGAACCCCTCCACATCGCCCGCGCGAATGT11.8867924528301887No Hit
CAACAAGACGCCACCTGCCACGCTGTCCGAAGTGTGCGCACAGGTCGACC11.8867924528301887No Hit
GCTCAAAACTGCGCAGATACCGAGGTGCGCCTTGCGAGCAGCGAGCCAGT11.8867924528301887No Hit
CCGAAGTGATTTGTGCCAATCGGACGCCGTACTTTTGGACTGACCGAGGT11.8867924528301887No Hit
ATAGCGAGCTGCCGATGATGCTGTACGTGTACGCGGACATCGTGATCCTG11.8867924528301887No Hit
CAACTGCGCCGCGCCGCCAATGAGCGTGCCCATCAGGCCCGCGAACGCAT11.8867924528301887No Hit
CTGCAAGCTCCCGCCAATTGGTTTCCACACGTTGTACAGACAAACTAATT11.8867924528301887No Hit
CACCGACAGCTGGGATGCCGCGCTGAAGAACTCGTTGCGCCAGGCGCCCG11.8867924528301887No Hit
GTGTCGGGATAATCCGGCTCCATGAATCAGATGACGGATTGGCTCAAGGT11.8867924528301887No Hit
CTTGATGTGGTCAGTCCCTAATTTTAGCTATTCTAACAGTCCTGTAGTGG11.8867924528301887No Hit
GATAGTAGATGCCGGCACTCACATCGCCCAAGCCCGCGGACGTCTTGTCT11.8867924528301887No Hit
CATGGCGCCCATGGCGGCGATCTCTGGCATGCACAGGCCCGAGCAGTAAT11.8867924528301887No Hit
CCCAGAGGACAGGCAAAACCGCTGGAATTCAAGGTGGGGGTGGCCGACTC11.8867924528301887No Hit
GTCTGCGTGCGTGGCGACCACGGTGCCGTTGCCCGGCAGCGACAGGCCGA11.8867924528301887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)