FastQCFastQC Report
Tue 10 May 2022
EGAF00003621876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548704
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA17170.31291916953402926Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12730.23200122470402987Clontech Universal Primer Mix Long (96% over 26bp)
TGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGG7510.13686796524173325No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA7180.13085379366653058No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG6990.12739108882020178Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT6710.12228815536245409Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG6160.11226453607044964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)