FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003621943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18876184
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1018187853.940340907886885TruSeq Adapter, Index 9 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG195987710.382803007217985TruSeq Adapter, Index 9 (100% over 51bp)
CTCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG590350.3127485936776204TruSeq Adapter, Index 9 (98% over 51bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC546540.2895394535251405TruSeq Adapter, Index 9 (98% over 51bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC437140.23158282415556025TruSeq Adapter, Index 9 (98% over 51bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC389100.20613276496986888TruSeq Adapter, Index 9 (98% over 51bp)
GATGGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC343070.1817475396510227TruSeq Adapter, Index 9 (98% over 51bp)
ATCTGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG250160.13252678613431612TruSeq Adapter, Index 9 (98% over 51bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC203240.10767006721273749TruSeq Adapter, Index 9 (98% over 51bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)