FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003621991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003621991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15752305
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG541763034.39261746138105TruSeq Adapter, Index 20 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGC5364113.405285766114864TruSeq Adapter, Index 20 (97% over 43bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG311710.19788215121533007TruSeq Adapter, Index 20 (97% over 41bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG249820.15859266310549472TruSeq Adapter, Index 20 (97% over 41bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG210240.13346618161596033TruSeq Adapter, Index 20 (97% over 41bp)
GATGGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG179350.11385635308610391TruSeq Adapter, Index 20 (97% over 41bp)
CTCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGC168550.10700021361953059TruSeq Adapter, Index 20 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)