FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003622019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003622019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10511042
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC324896830.91004678698839TruSeq Adapter, Index 4 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG6124195.826434715035864TruSeq Adapter, Index 4 (100% over 51bp)
GATCGGAAGAGCACACGTCGAACTCCAGTCACTGACCAATCTCGTATGCCG2227432.1191333837311275TruSeq Adapter, Index 4 (96% over 33bp)
ATCGGAAGAGCACACGTCGAACTCCAGTCACTGACCAATCTCGTATGCCGT433390.41231877867103944Illumina PCR Primer Index 4 (100% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)