FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003622024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003622024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5896492
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG454565677.09085334127478TruSeq Adapter, Index 16 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGC75863712.865903998513014TruSeq Adapter, Index 16 (97% over 39bp)
GAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTC91980.1559910536637716TruSeq Adapter, Index 16 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCG87930.14912256304256835TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGATCTCGTATGC73360.1244129560423384TruSeq Adapter, Index 16 (97% over 39bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG64200.10887829577314782TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATTTCGTATG62310.10567300014991965TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)