FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003622093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003622093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6735936
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG301473944.75605172020637TruSeq Adapter, Index 15 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGC4865677.223450460336915TruSeq Adapter, Index 15 (97% over 39bp)
GAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTC153800.22832758506019063TruSeq Adapter, Index 15 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATT130790.19416752178167965TruSeq Adapter, Index 15 (97% over 40bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATN69460.10311855694590923TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)