FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003622110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003622110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14047013
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC558642839.76950829332898TruSeq Adapter, Index 5 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG173814912.37379790279969TruSeq Adapter, Index 5 (100% over 51bp)
CTCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG535980.38156154621626676TruSeq Adapter, Index 5 (98% over 51bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC304680.2169002050471513TruSeq Adapter, Index 5 (98% over 51bp)
GAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT263140.1873280817779552TruSeq Adapter, Index 5 (100% over 51bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC243310.17321120155580405TruSeq Adapter, Index 5 (98% over 51bp)
ATCTGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG215460.15338492247426552TruSeq Adapter, Index 5 (98% over 51bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC209180.14891422112302452TruSeq Adapter, Index 5 (98% over 51bp)
GATGGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC189850.13515328846068556TruSeq Adapter, Index 5 (98% over 51bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC171590.12215408357634466TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)