FastQCFastQC Report
Wed 8 May 2024
EGAF00003622134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003622134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5789400
Total Bases295.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC239046741.2904100597644TruSeq Adapter, Index 9 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC3925606.7806681175942245TruSeq Adapter, Index 9 (100% over 50bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC69790.12054789788233668TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)