FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003622141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003622141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14363922
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC458778931.93966800989312TruSeq Adapter, Index 11 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG209723814.60073369933365TruSeq Adapter, Index 11 (100% over 51bp)
CTCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG639470.4451917797938474TruSeq Adapter, Index 11 (98% over 51bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC271250.18884118139878509TruSeq Adapter, Index 11 (98% over 51bp)
ATCTGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG262320.18262421642222787TruSeq Adapter, Index 11 (98% over 51bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC220300.1533703677867368TruSeq Adapter, Index 11 (98% over 51bp)
GATGGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC174080.121192526665071TruSeq Adapter, Index 11 (98% over 51bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC167130.116354015289139TruSeq Adapter, Index 11 (98% over 51bp)
CTCTGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG152840.10640547894927306TruSeq Adapter, Index 11 (100% over 47bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)