FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003623536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003623536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225276020
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA825600.020.36360712-13
GTATCAA1316450.020.2140549
ATCAACG868450.019.35064910-11
GGTATCA1408550.018.9427268
GTGGTAT1508700.017.8045966
TCAACGC773450.017.4474312-13
TGGTATC1648000.016.3599267
AGTGGTA1714650.015.674965
ACGCAGA1133850.015.09392114-15
AGAGTAC1392950.012.20580618-19
AAGCAGT2352700.012.0855011
AACGCAG1151300.012.04298914-15
CGCAGAG1166850.011.70027416-17
AGCAGTG2771500.010.1727662
CAGTGGT2802950.010.0464364
GCAGAGT1914400.09.1995716-17
TATCAAC1670400.08.66376610-11
CAGAGTA1791000.07.8677218-19
GCAGTGG3687200.07.74114473
GAGTACT1294500.07.658340520-21
Produced by FastQC (version 0.11.5)