FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003623552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003623552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242273987
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3449380.14237516964625674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2145550.035.6645139
GGTATCA2266750.033.7680558
GTGGTAT2351350.032.615246
AGTGGTA2521000.030.5741215
TGGTATC2572650.029.8671997
CAACGCA1644900.028.10493712-13
ATCAACG1761100.026.36371810-11
AAGCAGT3036500.025.9148351
TCAACGC1666400.022.95120612-13
ACGCAGA2085750.022.34673514-15
AGCAGTG3539350.022.29492
CAGTGGT3698700.021.1223834
AGAGTAC2259600.020.26052918-19
AACGCAG2059400.018.68436814-15
GCAGTGG4408500.017.9209483
CGCAGAG2185450.017.57742716-17
GCAGAGT2934950.016.03141616-17
GATCGGA390650.014.7293061
TATCAAC2725700.014.4487610-11
CAGAGTA2676650.014.31593418-19
Produced by FastQC (version 0.11.5)