FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003623553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003623553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242273987
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT113015384.664775669869997No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT3819190.15763929290518508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38754400.091.0328149
GGTATCA38860650.090.890288
GTGGTAT38939100.090.813436
AGTGGTA39103600.090.629125
TGGTATC39105250.090.2657557
AAGCAGT39595250.089.8087841
AGCAGTG40274150.088.313712
CAGTGGT40296350.087.945994
GCAGTGG41135100.086.414693
CAACGCA38364650.046.41444412-13
ATCAACG38443050.046.24577310-11
TCAACGC38328050.046.13662712-13
ACGCAGA38823150.046.01905414-15
AACGCAG38837100.045.62562614-15
AGAGTAC38421950.045.57444418-19
CGCAGAG39104450.045.3396916-17
TATCAAC39057450.045.23306710-11
GCAGAGT39417500.045.05020516-17
CAGAGTA38821750.044.9927518-19
GAGTACA24825150.044.35885220-21
Produced by FastQC (version 0.11.5)