FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003623556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003623556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231908302
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7856050.33875673842845005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2161300.044.137279
GTGGTAT2311400.041.2566766
GGTATCA2339350.040.8570868
TGGTATC2608400.036.650067
AGTGGTA2685050.035.6425485
AAGCAGT2953000.033.0160981
CAACGCA1778300.030.42717612-13
ATCAACG1904700.028.60050410-11
AGCAGTG3532100.027.6064682
TCAACGC1762250.027.0474212-13
CAGTGGT3757800.025.763974
AGAGTAC2357000.022.95919818-19
ACGCAGA2392750.022.79432114-15
GCAGTGG4615600.021.199473
CGCAGAG2333950.020.47522716-17
GATCGGA487600.020.3249931
AACGCAG2417500.019.91192414-15
GAGTACT2093050.019.78656820-21
TATCAAC2558100.019.03694310-11
GTACTTT2233400.018.4406522-23
Produced by FastQC (version 0.11.5)