FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003623567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003623567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216121743
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63467422.9366513113861012No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT2623370.12138389981428199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20077250.088.973239
GTGGTAT20169400.088.5423056
GGTATCA20225050.088.3454748
AGTGGTA20398500.087.577545
TGGTATC20463950.087.15487
AAGCAGT20790200.086.56291
AGCAGTG21446350.083.909292
CAGTGGT21447500.083.349814
GCAGTGG22334100.080.5310753
CAACGCA19780050.045.7287412-13
TCAACGC19783500.045.45233512-13
ATCAACG19971650.045.32455410-11
ACGCAGA20187700.044.84964814-15
AGAGTAC19744400.044.64658718-19
AACGCAG20298600.044.3738814-15
CGCAGAG20420200.044.03907816-17
CAGAGTA20126900.043.8482618-19
GCAGAGT20510850.043.68174716-17
TATCAAC20788850.043.1168710-11
GAGTACA12264000.042.83891320-21
Produced by FastQC (version 0.11.5)