FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003623570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003623570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252536878
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3466470.13726589270656936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2229000.034.3530169
GGTATCA2464900.031.0903478
GTGGTAT2712200.028.6546346
AGTGGTA2754000.028.1007695
CAACGCA1648350.027.89326512-13
TGGTATC2929900.026.4687967
ATCAACG1804450.025.5994810-11
AAGCAGT3221000.024.7802371
TCAACGC1662050.023.0401712-13
AGCAGTG3677750.021.5801942
ACGCAGA2233850.020.78826114-15
AGAGTAC2290650.019.84912718-19
CAGTGGT4031000.019.5411384
AACGCAG2229100.017.30757314-15
CGCAGAG2252550.017.18246716-17
GCAGTGG4639450.017.1335373
GCAGAGT2870250.016.2173316-17
TATCAAC2799750.014.16474210-11
CAGAGTA2856050.013.45272418-19
GATCGGA410100.013.4422521
Produced by FastQC (version 0.11.5)